Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CLK_RS12670 Genome accession   NC_010520
Coordinates   2739416..2740468 (-) Length   350 a.a.
NCBI ID   WP_012344954.1    Uniprot ID   -
Organism   Clostridium botulinum A3 str. Loch Maree     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2734416..2745468
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLK_RS12650 (CLK_1918) ftsZ 2734940..2736049 (-) 1110 WP_012344963.1 cell division protein FtsZ -
  CLK_RS12655 (CLK_1919) ftsA 2736072..2737325 (-) 1254 WP_012344007.1 cell division protein FtsA -
  CLK_RS12660 (CLK_1920) - 2737482..2738756 (-) 1275 WP_012343097.1 glycosyltransferase family 2 protein -
  CLK_RS21840 (CLK_1921) - 2739194..2739337 (+) 144 WP_012100440.1 hypothetical protein -
  CLK_RS12670 (CLK_1922) pilT 2739416..2740468 (-) 1053 WP_012344954.1 type IV pilus twitching motility protein PilT Machinery gene
  CLK_RS12675 (CLK_1923) aroE 2740470..2741246 (-) 777 WP_012344116.1 shikimate dehydrogenase -
  CLK_RS12680 (CLK_1924) sigK 2741385..2742089 (-) 705 WP_012047887.1 RNA polymerase sporulation sigma factor SigK -
  CLK_RS12685 (CLK_1925) - 2742513..2742692 (-) 180 WP_012341846.1 aspartyl-phosphate phosphatase Spo0E family protein -
  CLK_RS20740 (CLK_1926) - 2743136..2743273 (-) 138 WP_012344550.1 hypothetical protein -
  CLK_RS12690 - 2743662..2743916 (+) 255 WP_041173445.1 hypothetical protein -
  CLK_RS22370 - 2744123..2744422 (-) 300 Protein_2482 hypothetical protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39053.12 Da        Isoelectric Point: 8.6835

>NTDB_id=30424 CLK_RS12670 WP_012344954.1 2739416..2740468(-) (pilT) [Clostridium botulinum A3 str. Loch Maree]
MKQLKELLEKTINFGASDLHLSVGVEPTIRINGQLSKIFKEKLTPENTNNYAKEILGEEFEDLLKKGEIDTSYSISGLGR
FRVSVFKQRGSIAISLRVVSLKIPNLNELKLPDIVKELTNKKRGLILVTGPTGSGKSTTLAAMINEINNTRSNHIITLED
PIEFLHKHNKCIINQREIGRDTFSYKSALKAALREDPDVILIGELRDLEDISIALTAAETGHLVFSTLHTIGGAKTIDRI
VNVFPPHQQEQIRIQVASVLEGIISQQLVHKIDGGRIAALETMVTTNAIRNMIREGKTYQIESSIQTGSKYGMKTMDMSL
AELYKREIISYDTALNYSVDKDLITKIINL

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=30424 CLK_RS12670 WP_012344954.1 2739416..2740468(-) (pilT) [Clostridium botulinum A3 str. Loch Maree]
TTGAAACAATTAAAAGAATTATTGGAAAAAACTATTAACTTTGGTGCTTCAGATTTACATTTATCTGTAGGGGTAGAACC
AACTATAAGAATAAATGGACAATTAAGTAAAATATTTAAAGAAAAGCTTACACCAGAAAATACTAATAATTATGCCAAAG
AAATATTAGGTGAAGAATTTGAAGATTTATTGAAAAAAGGAGAAATAGATACATCTTATTCTATATCGGGCTTAGGTAGA
TTTAGAGTAAGTGTGTTTAAACAAAGGGGAAGTATAGCTATTTCTTTAAGGGTTGTAAGTTTAAAGATTCCTAACTTAAA
TGAACTAAAATTGCCTGATATAGTAAAGGAATTAACCAATAAAAAAAGAGGCCTTATTTTAGTAACGGGTCCTACAGGTT
CAGGTAAAAGCACTACTTTAGCAGCTATGATTAATGAAATTAATAATACTAGGTCTAATCATATAATAACCTTAGAAGAT
CCAATAGAATTTTTACATAAACATAACAAATGTATAATAAATCAAAGAGAGATAGGTAGGGATACTTTTAGCTATAAAAG
TGCTTTAAAAGCAGCATTAAGAGAAGATCCGGATGTAATATTAATAGGAGAGCTTAGAGATTTAGAAGATATTTCTATAG
CTTTAACAGCTGCAGAAACAGGGCATTTAGTATTTTCTACTTTGCATACTATAGGAGGGGCTAAAACTATAGATAGAATT
GTTAATGTATTTCCGCCACATCAACAGGAACAGATAAGAATACAAGTAGCAAGTGTGTTAGAAGGAATAATAAGCCAACA
ATTAGTACATAAAATTGATGGAGGAAGAATAGCGGCCTTAGAGACCATGGTAACTACAAATGCCATAAGAAATATGATAA
GAGAAGGAAAAACTTATCAAATAGAATCTTCTATACAAACTGGATCTAAATATGGAATGAAAACTATGGATATGTCCTTA
GCAGAGCTATATAAAAGAGAAATTATAAGCTATGATACTGCATTAAATTATTCAGTAGATAAGGATTTAATTACAAAAAT
AATTAATTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(92-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

50.751

95.143

0.483
  pilT Legionella pneumophila strain Lp02

48.406

98.571

0.477
  pilT Legionella pneumophila strain ERS1305867

48.406

98.571

0.477
  pilT Pseudomonas aeruginosa PAK

49.405

96

0.474
  pilT Pseudomonas stutzeri DSM 10701

48.81

96

0.469
  pilT Vibrio cholerae strain A1552

49.697

94.286

0.469
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.697

94.286

0.469
  pilT Acinetobacter baumannii strain A118

49.249

95.143

0.469
  pilT Acinetobacter baumannii D1279779

49.249

95.143

0.469
  pilT Acinetobacter nosocomialis M2

49.249

95.143

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.977

99.429

0.457
  pilT Neisseria gonorrhoeae MS11

47.463

95.714

0.454
  pilT Neisseria meningitidis 8013

47.463

95.714

0.454
  pilU Acinetobacter baylyi ADP1

41.124

96.571

0.397
  pilU Pseudomonas stutzeri DSM 10701

41.194

95.714

0.394
  pilU Vibrio cholerae strain A1552

38.348

96.857

0.371

Multiple sequence alignment