Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MG068_RS02480 Genome accession   NZ_CP031167
Coordinates   535838..536965 (-) Length   375 a.a.
NCBI ID   WP_032127971.1    Uniprot ID   A0AA43AM75
Organism   Stenotrophomonas sp. ASS1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 530838..541965
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MG068_RS02460 (MG068_02450) - 531271..532260 (+) 990 WP_132809096.1 fumarylacetoacetate hydrolase family protein -
  MG068_RS02465 (MG068_02455) maiA 532275..532943 (+) 669 WP_132809098.1 maleylacetoacetate isomerase -
  MG068_RS02470 (MG068_02460) - 532989..534449 (-) 1461 WP_132811097.1 UdgX family uracil-DNA binding protein -
  MG068_RS02475 (MG068_02465) - 534430..535707 (-) 1278 WP_132809100.1 putative DNA modification/repair radical SAM protein -
  MG068_RS02480 (MG068_02470) pilU 535838..536965 (-) 1128 WP_032127971.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MG068_RS02485 (MG068_02475) - 537087..537452 (+) 366 WP_049398394.1 DUF4398 domain-containing protein -
  MG068_RS02490 (MG068_02480) - 537449..538333 (+) 885 WP_132809102.1 hypothetical protein -
  MG068_RS02495 (MG068_02485) - 538658..538894 (+) 237 WP_004154075.1 YdcH family protein -
  MG068_RS02500 (MG068_02490) - 539195..540565 (+) 1371 WP_032127973.1 pyridoxal-phosphate dependent enzyme -
  MG068_RS02505 (MG068_02495) - 540689..541864 (+) 1176 WP_132809103.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41453.78 Da        Isoelectric Point: 6.3741

>NTDB_id=303826 MG068_RS02480 WP_032127971.1 535838..536965(-) (pilU) [Stenotrophomonas sp. ASS1]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEGSLEEGMESFDQCL
FRLAKAGIIEQEEALRAADSRDGLALKFRLSEGSSGEHDPYADFSAGQPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=303826 MG068_RS02480 WP_032127971.1 535838..536965(-) (pilU) [Stenotrophomonas sp. ASS1]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTCCTGACCACCGGGGCACCGGTCTA
CATCAAGATCGAGGGGAAGCTTTATCCGCTGGGCAACACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTACTCAC
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAGCTCAACATGGCCATCGCCCTGCCCGATGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTCATCCGCGCCATCCGCAGCCGCATCCCGAGCATCGA
GGAGCTGAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGGTCCACCGGTT
CCGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGGCTGGACACCCATGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGTGAAGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCGGAAACGATGGAGGCGG
CGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGTGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAGGGCCGTCGCCTGCCGGCCACCGAGGTGCTGATCAACACGCCGATGATCCGTGACCTGCTGC
GTCGCGGCCAGGTGCACGAGATCAAGGCGGCGATGGAAGGCTCGCTGGAGGAAGGCATGGAGAGTTTCGACCAGTGCCTG
TTCCGTCTGGCCAAGGCTGGCATCATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGATGGCCTGGCCCTGAA
GTTCCGCCTGTCCGAAGGCAGCAGCGGCGAGCACGACCCGTATGCGGATTTCTCGGCCGGGCAGCCAAGCATCACCCACG
GCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

95.733

0.491
  pilU Acinetobacter baylyi ADP1

49.435

94.4

0.467
  pilU Vibrio cholerae strain A1552

49.153

94.4

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.81

95.467

0.437
  pilT Pseudomonas aeruginosa PAK

42.899

90.133

0.387
  pilT Acinetobacter baylyi ADP1

41.765

90.667

0.379
  pilT Acinetobacter baumannii strain A118

41.716

90.133

0.376
  pilT Acinetobacter nosocomialis M2

41.716

90.133

0.376
  pilT Acinetobacter baumannii D1279779

41.716

90.133

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.42

90.133

0.373
  pilT Legionella pneumophila strain Lp02

40.828

90.133

0.368
  pilT Legionella pneumophila strain ERS1305867

40.828

90.133

0.368
  pilT Vibrio cholerae strain A1552

40.841

88.8

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.8

0.363
  pilT Neisseria meningitidis 8013

39.193

92.533

0.363
  pilT Neisseria gonorrhoeae MS11

39.193

92.533

0.363

Multiple sequence alignment