Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DAUD_RS04795 Genome accession   NC_010424
Coordinates   1009815..1010933 (+) Length   372 a.a.
NCBI ID   WP_012302050.1    Uniprot ID   B1I3G0
Organism   Candidatus Desulforudis audaxviator MP104C     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1004815..1015933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DAUD_RS04780 (Daud_0948) lspA 1006558..1007019 (+) 462 WP_012302047.1 signal peptidase II -
  DAUD_RS04785 (Daud_0949) - 1007006..1007935 (+) 930 WP_012302048.1 RluA family pseudouridine synthase -
  DAUD_RS04790 (Daud_0950) - 1008250..1009578 (-) 1329 WP_012302049.1 ISNCY family transposase -
  DAUD_RS04795 (Daud_0951) pilT 1009815..1010933 (+) 1119 WP_012302050.1 type IV pilus twitching motility protein PilT Machinery gene
  DAUD_RS04800 (Daud_0952) - 1010982..1012190 (+) 1209 WP_012302051.1 type II secretion system F family protein -
  DAUD_RS04805 (Daud_0953) pilM 1012231..1013268 (+) 1038 WP_012302052.1 type IV pilus biogenesis protein PilM -
  DAUD_RS04810 (Daud_0954) - 1013265..1013867 (+) 603 WP_012302053.1 PilN domain-containing protein -
  DAUD_RS04815 (Daud_0955) - 1013864..1014442 (+) 579 WP_012302054.1 type 4a pilus biogenesis protein PilO -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41528.82 Da        Isoelectric Point: 7.6029

>NTDB_id=30165 DAUD_RS04795 WP_012302050.1 1009815..1010933(+) (pilT) [Candidatus Desulforudis audaxviator MP104C]
MDSHGGKEYPIDFDTLLRITVERQGSDLHLTAGLPPVVRIHGELVPLDYPPLKPSDIEQLVLPRLEPSYRAILDEKWELD
FSYSVAGVSRFRGNIMRQRGSYSAVLRVVPYKAPALEELGLPPAVRNLCFLPRGLVLVTGPTGSGKSTTLAAMIDLINRE
RRVNVVTVEDPIEFLHRHNKAIVRQREVGSDTRSFADALRHVLRADPDVILIGEMRDLESIAIALTAAETGHLVFSTLHT
QTAPLTIHRIVDVFQEHQRDQIRQQLADSLQGVIAQQLVPRADGKGRVAAVELMLASPAVRALIREGKEHQMYSVIQTSR
QQGMQTMDQALAELYTAGKISREAALERCVDRTELERLMQKGVPGSMDRRLK

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=30165 DAUD_RS04795 WP_012302050.1 1009815..1010933(+) (pilT) [Candidatus Desulforudis audaxviator MP104C]
ATGGACAGTCATGGGGGAAAGGAATACCCTATTGATTTTGACACATTATTGAGAATCACCGTGGAACGGCAGGGTTCGGA
CTTGCACCTGACGGCCGGTTTGCCCCCGGTGGTCCGGATTCACGGCGAGCTGGTTCCCCTGGACTATCCCCCGCTGAAAC
CGTCCGACATAGAGCAATTGGTCCTGCCCCGGCTGGAACCGTCCTACCGAGCCATACTCGACGAGAAATGGGAACTGGAC
TTTTCCTATTCCGTGGCCGGGGTGAGCCGGTTCCGCGGGAACATCATGCGCCAGCGGGGCAGCTACTCCGCGGTTTTGCG
GGTGGTGCCCTACAAGGCTCCGGCTCTCGAGGAGTTGGGCCTGCCGCCGGCGGTGCGCAATCTCTGCTTTCTCCCGCGCG
GGCTGGTACTGGTCACCGGGCCGACCGGGAGCGGCAAATCCACCACACTGGCCGCCATGATCGACCTCATCAACCGGGAA
CGACGCGTGAATGTAGTCACCGTGGAAGATCCGATTGAGTTCCTGCACCGGCACAACAAGGCCATCGTCCGGCAGCGGGA
GGTGGGGAGCGACACCCGCTCCTTTGCGGATGCCCTGCGGCACGTTCTACGCGCCGACCCCGATGTGATCCTCATCGGCG
AGATGCGTGATCTCGAGAGCATCGCCATCGCCCTGACGGCGGCCGAAACCGGCCACCTGGTGTTTTCGACCCTGCACACC
CAGACCGCGCCGCTTACCATCCACCGCATTGTAGACGTGTTCCAGGAACACCAGCGCGATCAAATACGCCAACAGTTGGC
CGACTCCCTGCAGGGGGTGATCGCCCAGCAGTTGGTGCCCAGGGCGGACGGCAAAGGGCGGGTGGCCGCCGTCGAATTGA
TGCTGGCCTCACCGGCGGTGCGCGCCCTGATCCGGGAGGGCAAGGAGCACCAGATGTACAGTGTCATCCAGACCAGTAGG
CAGCAGGGTATGCAGACCATGGACCAGGCGCTGGCCGAACTGTACACCGCCGGGAAAATCAGCCGCGAGGCGGCCCTGGA
GCGGTGCGTGGACCGGACCGAGTTGGAGCGCCTGATGCAAAAAGGGGTCCCTGGCTCCATGGACCGACGGCTGAAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-281)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B1I3G0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.831

95.43

0.495
  pilT Acinetobacter baylyi ADP1

51.964

88.978

0.462
  pilT Acinetobacter baumannii strain A118

51.343

90.054

0.462
  pilT Acinetobacter nosocomialis M2

51.343

90.054

0.462
  pilT Acinetobacter baumannii D1279779

51.343

90.054

0.462
  pilT Pseudomonas aeruginosa PAK

50.442

91.129

0.46
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.212

88.71

0.454
  pilT Vibrio cholerae strain A1552

51.212

88.71

0.454
  pilT Legionella pneumophila strain Lp02

49.271

92.204

0.454
  pilT Legionella pneumophila strain ERS1305867

49.271

92.204

0.454
  pilT Pseudomonas stutzeri DSM 10701

49.263

91.129

0.449
  pilT Neisseria gonorrhoeae MS11

47.904

89.785

0.43
  pilT Neisseria meningitidis 8013

47.904

89.785

0.43
  pilU Pseudomonas stutzeri DSM 10701

42.165

94.355

0.398
  pilU Vibrio cholerae strain A1552

42.69

91.935

0.392
  pilU Acinetobacter baylyi ADP1

40.588

91.398

0.371

Multiple sequence alignment