Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CX698_RS18925 Genome accession   NZ_CP030787
Coordinates   3750987..3751967 (-) Length   326 a.a.
NCBI ID   WP_113640687.1    Uniprot ID   -
Organism   Escherichia albertii strain 2013C-4143     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3745987..3756967
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CX698_RS18895 - 3746642..3747139 (+) 498 WP_010336943.1 SprT family zinc-dependent metalloprotease -
  CX698_RS18900 endA 3747234..3747941 (+) 708 WP_002461298.1 deoxyribonuclease I -
  CX698_RS18905 rsmE 3748021..3748752 (+) 732 WP_002461297.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CX698_RS18910 gshB 3748765..3749715 (+) 951 WP_000593300.1 glutathione synthase -
  CX698_RS18915 - 3749824..3750387 (+) 564 WP_010336945.1 YqgE/AlgH family protein -
  CX698_RS18920 ruvX 3750387..3750803 (+) 417 WP_000017099.1 Holliday junction resolvase RuvX -
  CX698_RS18925 pilT 3750987..3751967 (-) 981 WP_113640687.1 type IV pilus twitching motility protein PilT Machinery gene
  CX698_RS18930 yggS 3751985..3752689 (+) 705 WP_000997809.1 pyridoxal phosphate homeostasis protein -
  CX698_RS18935 yggT 3752707..3753273 (+) 567 WP_001094839.1 osmotic shock tolerance protein YggT -
  CX698_RS18940 yggU 3753270..3753560 (+) 291 WP_001277225.1 DUF167 family protein YggU -
  CX698_RS18945 - 3753568..3754161 (+) 594 WP_001174776.1 XTP/dITP diphosphatase -
  CX698_RS18950 hemW 3754154..3755290 (+) 1137 WP_113640688.1 radical SAM family heme chaperone HemW -
  CX698_RS18955 - 3755355..3756362 (-) 1008 WP_113640689.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35996.10 Da        Isoelectric Point: 5.3850

>NTDB_id=301495 CX698_RS18925 WP_113640687.1 3750987..3751967(-) (pilT) [Escherichia albertii strain 2013C-4143]
MNMEEIVALSVKHNVSDLHLCSAWPARWCIRGRMEAAPFDTPDVEELLREWLDDDQRALLLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLNNENGLILVTGATGSGKSTTLAAMVGYLNQHTDAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPTQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=301495 CX698_RS18925 WP_113640687.1 3750987..3751967(-) (pilT) [Escherichia albertii strain 2013C-4143]
ATGAATATGGAAGAAATTGTGGCTCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGTAGCGCCTGGCCTGCACG
CTGGTGTATTCGCGGGAGGATGGAAGCTGCGCCGTTTGATACGCCGGACGTTGAAGAGCTGCTAAGGGAATGGCTGGATG
ACGATCAGCGAGCGTTATTGCTGGAGAATGGGCAGTTGGACTTTGCCGTGTCGTTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGCATTTCGCTGGCGTTACGGTTGTTGCCTTCGCATTGTCCGCAACTCGAACAACTTGG
TGCGCCGACGGTATTACCGGAACTACTCAATAACGAGAATGGACTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CCACCACACTGGCGGCGATGGTTGGTTATCTCAATCAGCATACCGATGCGCATATTCTTACGCTGGAAGATCCTGTGGAA
TATCTTTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTTGCATCGGGATTACG
TGCGGCATTGCGGGAAGATCCCGATGTAATTTTGCTTGGCGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGTTGACGG
CGGCAGAAACAGGTCATCTGGTGCTGGCAACCTTGCATACTCGCGGAGCAGCTCAGGCGGTCGAGCGGCTGGTGGATTCG
TTTCCGACTCAGGAAAAAGATCCTGTGCGTAATCAACTGGCGGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACACACCTGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACTTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

44.343

100

0.445
  pilT Legionella pneumophila strain Lp02

44.343

100

0.445
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

40.379

97.239

0.393
  pilU Pseudomonas stutzeri DSM 10701

38.253

100

0.39

Multiple sequence alignment