Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DOP62_RS06295 Genome accession   NZ_CP030139
Coordinates   1315592..1316680 (-) Length   362 a.a.
NCBI ID   WP_208676345.1    Uniprot ID   -
Organism   Synechococcus elongatus PCC 11801     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1310592..1321680
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DOP62_RS06275 (DOP62_05625) dusA 1311522..1312535 (-) 1014 WP_208676897.1 tRNA dihydrouridine(20/20a) synthase DusA -
  DOP62_RS06280 (DOP62_05620) - 1312562..1313794 (+) 1233 WP_208676351.1 NAD(P)/FAD-dependent oxidoreductase -
  DOP62_RS06285 (DOP62_05615) - 1313774..1314331 (-) 558 WP_208676349.1 hypothetical protein -
  DOP62_RS06290 (DOP62_05610) - 1314336..1315568 (-) 1233 WP_208676347.1 type II secretion system F family protein -
  DOP62_RS06295 (DOP62_05605) pilT 1315592..1316680 (-) 1089 WP_208676345.1 type IV pilus twitching motility protein PilT Machinery gene
  DOP62_RS06300 (DOP62_05600) pilB/pilB1 1316752..1318752 (-) 2001 WP_208676343.1 GspE/PulE family protein Machinery gene
  DOP62_RS06305 (DOP62_05595) grpE 1319038..1319661 (+) 624 WP_338462949.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 362 a.a.        Molecular weight: 39853.95 Da        Isoelectric Point: 6.3637

>NTDB_id=299652 DOP62_RS06295 WP_208676345.1 1315592..1316680(-) (pilT) [Synechococcus elongatus PCC 11801]
MELMIEDLMEKLVEMGGSDLHLSVGLPPYFRISGKLTPIGDEPMGEEQCQRLIFSMLNNTQRKMLEQNWELDCSYGIRGL
ARFRVNVYKDKGTYAACLRALSAKVPEFNDLGLPQVVREMSEKPRGLILVTGPTGSGKTTTLAAIIDLINRSRAEHIITV
EDPIEFVYQPAKSIIHQRQLGEDTKSFANALRAALREDPDIILVGEMRDLETIQLAITAAETGHLVFGTLHTSSAAQTVD
RIIDVFPAEKQTQVRVQLSNSLVAVFSQTLVPKRNARPGSFGRVLAQEIMIVTPAVSNLIREGKTAQIYSQIQTGGKLGM
QTLEKALADLYKAGEITFEAAASKTSRPDEMQRLIGNVSIAA

Nucleotide


Download         Length: 1089 bp        

>NTDB_id=299652 DOP62_RS06295 WP_208676345.1 1315592..1316680(-) (pilT) [Synechococcus elongatus PCC 11801]
ATGGAACTAATGATCGAAGACCTAATGGAAAAGCTCGTGGAGATGGGAGGATCTGACCTCCATCTTTCTGTTGGCTTACC
TCCTTATTTTCGAATCAGTGGAAAACTTACCCCCATCGGGGATGAACCAATGGGTGAGGAACAGTGTCAGCGCTTGATCT
TCAGTATGTTGAACAACACGCAACGCAAAATGCTGGAGCAAAATTGGGAGCTTGATTGTTCCTATGGGATTCGTGGACTC
GCTCGTTTCCGGGTCAATGTTTACAAAGATAAAGGAACCTATGCCGCTTGCTTACGGGCTTTGAGTGCCAAGGTGCCGGA
GTTTAACGACCTAGGGCTACCTCAAGTGGTGCGCGAGATGAGCGAAAAACCAAGGGGGCTCATTCTCGTCACTGGCCCTA
CAGGGTCTGGAAAAACAACAACGTTGGCAGCCATTATTGATTTGATTAATCGCAGCCGTGCTGAACATATCATTACGGTT
GAAGACCCCATTGAATTTGTCTATCAACCGGCAAAAAGCATCATTCACCAGCGGCAGCTTGGAGAAGATACGAAGAGTTT
TGCTAATGCCCTGCGAGCAGCGCTTCGAGAAGATCCTGACATCATCCTTGTGGGTGAGATGCGAGACTTGGAAACGATTC
AGTTAGCTATCACTGCTGCAGAAACTGGTCACCTCGTTTTTGGTACTTTGCACACCAGCTCCGCTGCTCAAACCGTTGAC
CGGATTATCGATGTCTTTCCTGCTGAAAAACAGACGCAGGTAAGGGTTCAGTTGTCGAATTCACTAGTTGCGGTCTTCAG
TCAAACCCTTGTCCCCAAACGCAACGCTCGACCTGGTAGCTTTGGTCGTGTCCTTGCCCAAGAGATCATGATTGTTACAC
CAGCAGTGTCCAACTTAATTCGCGAAGGAAAAACCGCACAAATTTACTCTCAAATTCAGACGGGCGGAAAACTAGGCATG
CAGACCCTAGAGAAAGCTTTGGCTGATCTTTATAAGGCAGGTGAAATCACTTTTGAAGCAGCTGCCTCTAAAACATCTAG
GCCCGATGAGATGCAACGGTTGATTGGCAATGTTTCAATTGCTGCCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(48-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

53.314

95.856

0.511
  pilT Pseudomonas aeruginosa PAK

53.333

95.304

0.508
  pilT Legionella pneumophila strain ERS1305867

53.333

95.304

0.508
  pilT Acinetobacter baylyi ADP1

53.333

95.304

0.508
  pilT Legionella pneumophila strain Lp02

53.333

95.304

0.508
  pilT Acinetobacter baumannii strain A118

52.464

95.304

0.5
  pilT Acinetobacter baumannii D1279779

52.464

95.304

0.5
  pilT Acinetobacter nosocomialis M2

52.464

95.304

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.577

98.066

0.486
  pilT Neisseria meningitidis 8013

50.143

96.409

0.483
  pilT Neisseria gonorrhoeae MS11

50.143

96.409

0.483
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.118

93.923

0.461
  pilT Vibrio cholerae strain A1552

49.118

93.923

0.461
  pilU Pseudomonas stutzeri DSM 10701

39.886

96.961

0.387
  pilU Acinetobacter baylyi ADP1

37.536

96.409

0.362

Multiple sequence alignment