Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C9I47_RS03855 Genome accession   NZ_CP029843
Coordinates   853665..854777 (-) Length   370 a.a.
NCBI ID   WP_111265574.1    Uniprot ID   A0A2U9T622
Organism   Marilutibacter maris strain HZ9B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 848665..859777
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C9I47_RS03830 (C9I47_0678) - 849050..849868 (+) 819 WP_111267944.1 DUF3050 domain-containing protein -
  C9I47_RS03835 (C9I47_0679) - 850066..850746 (-) 681 WP_111265570.1 acyl-homoserine-lactone synthase -
  C9I47_RS03840 (C9I47_0680) - 850851..851624 (-) 774 WP_111265571.1 LuxR family transcriptional regulator -
  C9I47_RS03845 (C9I47_0681) - 851826..852806 (+) 981 WP_111265572.1 fumarylacetoacetate hydrolase family protein -
  C9I47_RS03850 (C9I47_0682) maiA 852965..853621 (+) 657 WP_111265573.1 maleylacetoacetate isomerase -
  C9I47_RS03855 (C9I47_0683) pilU 853665..854777 (-) 1113 WP_111265574.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C9I47_RS03860 (C9I47_0684) - 854880..855260 (+) 381 WP_223250267.1 DUF4398 domain-containing protein -
  C9I47_RS03865 (C9I47_0685) - 855257..856096 (+) 840 WP_145985418.1 hypothetical protein -
  C9I47_RS03870 (C9I47_0686) - 856491..856706 (+) 216 WP_111267946.1 YdcH family protein -
  C9I47_RS03875 (C9I47_0687) - 856999..858375 (+) 1377 WP_111265576.1 pyridoxal-phosphate dependent enzyme -
  C9I47_RS03880 (C9I47_0688) - 858535..859713 (+) 1179 WP_111265577.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40873.05 Da        Isoelectric Point: 6.1500

>NTDB_id=296271 C9I47_RS03855 WP_111265574.1 853665..854777(-) (pilU) [Marilutibacter maris strain HZ9B]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPSFERDLELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSVIPSIEELQLPQVLKDIIMAPRGLVLIVGSTGSGKSTTLASMIDHRNSSTSGHILTIED
PIEYLHKHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAATMEAAIAFAETGHLCLATLHSNNADQTLERI
LNFFNESAHKNVLMNLALNLKAVVSQRLVVGTDGRRLPAAEVLINTPHIRDLMRRGQVHEIKHAMEESLEEGMETFDQCL
FRLVKEGKVDMEEALRAADSRDGLALKFRLSEGSSAEHDPYADVFDANAH

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=296271 C9I47_RS03855 WP_111265574.1 853665..854777(-) (pilU) [Marilutibacter maris strain HZ9B]
ATGGACATCGGCTATTTTCTCAAGCTGATGACGGAAAAGAACGCGTCGGACATGTTCCTGACCACCGGCGCGCCGGTCTA
CATCAAGGTCGAGGGCAAGCTTTACCCGCTCGGCAACACCGGCCTGCCGCCGGGGATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAGGGCCAGGTGCCCTCGTTCGAGCGCGACCTGGAGCTGAACATGGCGATCGCCCTGCCCGACGCCGGACGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAGGTCGGCATGGTGATCCGCGCGATCCGCAGCGTGATCCCCTCGATCGA
GGAGCTGCAGCTGCCGCAGGTGCTCAAGGACATCATCATGGCCCCGCGCGGGCTGGTGCTGATCGTCGGCTCGACCGGTT
CGGGCAAGTCGACCACGCTGGCCTCGATGATCGACCATCGCAACAGCAGCACCTCCGGTCACATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACAAGCACAAGAAGTCGATCGTCAACCAGCGCGAGGTCGGCCTGGACACCCACGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTCGACGCGGCGACGATGGAGGCGG
CGATCGCGTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCTGATCAGACCCTGGAGCGCATC
CTCAATTTCTTCAACGAGTCGGCGCACAAGAACGTGTTGATGAACCTGGCGCTGAACCTCAAGGCGGTGGTCAGCCAGCG
CCTGGTGGTGGGCACCGACGGCCGCCGCCTGCCGGCCGCCGAGGTCCTGATCAACACCCCGCACATCCGCGACCTGATGC
GCCGCGGCCAGGTCCACGAGATCAAGCACGCGATGGAGGAGTCGCTGGAGGAAGGCATGGAGACCTTCGACCAGTGCCTG
TTCCGCCTGGTCAAGGAGGGCAAGGTCGACATGGAGGAGGCGCTGCGCGCGGCCGACTCCCGCGACGGCCTGGCCCTGAA
GTTCCGCCTGTCCGAGGGCTCCAGCGCCGAGCACGACCCTTACGCGGACGTGTTCGACGCCAACGCGCACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U9T622

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.532

97.027

0.5
  pilU Acinetobacter baylyi ADP1

49.153

95.676

0.47
  pilU Vibrio cholerae strain A1552

48.87

95.676

0.468
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.221

92.973

0.43
  pilT Pseudomonas aeruginosa PAK

41.716

91.351

0.381
  pilT Acinetobacter baumannii strain A118

41.42

91.351

0.378
  pilT Acinetobacter nosocomialis M2

41.42

91.351

0.378
  pilT Acinetobacter baumannii D1279779

41.42

91.351

0.378
  pilT Acinetobacter baylyi ADP1

40.882

91.892

0.376
  pilT Legionella pneumophila strain Lp02

40.828

91.351

0.373
  pilT Legionella pneumophila strain ERS1305867

40.828

91.351

0.373
  pilT Pseudomonas stutzeri DSM 10701

40.533

91.351

0.37
  pilT Neisseria meningitidis 8013

39.193

93.784

0.368
  pilT Neisseria gonorrhoeae MS11

39.193

93.784

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.541

90

0.365
  pilT Vibrio cholerae strain A1552

40.541

90

0.365

Multiple sequence alignment