Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AN232_RS08580 Genome accession   NZ_CP029727
Coordinates   1697268..1698248 (+) Length   326 a.a.
NCBI ID   WP_110496882.1    Uniprot ID   -
Organism   Citrobacter sp. CRE-46 strain AR_0157     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1692268..1703248
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AN232_RS08550 (AN232_08550) ansB 1692781..1693827 (+) 1047 WP_038633619.1 L-asparaginase 2 -
  AN232_RS08555 (AN232_08555) hemW 1693944..1695080 (-) 1137 WP_110496880.1 radical SAM family heme chaperone HemW -
  AN232_RS08560 (AN232_08560) - 1695073..1695666 (-) 594 WP_038633614.1 XTP/dITP diphosphatase -
  AN232_RS08565 (AN232_08565) yggU 1695674..1695964 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  AN232_RS08570 (AN232_08570) - 1695961..1696527 (-) 567 WP_038633609.1 YggT family protein -
  AN232_RS08575 (AN232_08575) - 1696546..1697250 (-) 705 WP_069324800.1 YggS family pyridoxal phosphate-dependent enzyme -
  AN232_RS08580 (AN232_08580) pilT 1697268..1698248 (+) 981 WP_110496882.1 type IV pilus twitching motility protein PilT Machinery gene
  AN232_RS08585 (AN232_08585) ruvX 1698245..1698661 (-) 417 WP_038633601.1 Holliday junction resolvase RuvX -
  AN232_RS08590 (AN232_08590) - 1698661..1699224 (-) 564 WP_032940453.1 YqgE/AlgH family protein -
  AN232_RS08595 (AN232_08595) gshB 1699336..1700283 (-) 948 WP_038633598.1 glutathione synthase -
  AN232_RS08600 (AN232_08600) rsmE 1700296..1701027 (-) 732 WP_110496885.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AN232_RS08605 (AN232_08605) endA 1701102..1701809 (-) 708 WP_038633590.1 deoxyribonuclease I -
  AN232_RS08610 (AN232_08610) - 1701904..1702401 (-) 498 WP_038633587.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35896.22 Da        Isoelectric Point: 6.8708

>NTDB_id=295656 AN232_RS08580 WP_110496882.1 1697268..1698248(+) (pilT) [Citrobacter sp. CRE-46 strain AR_0157]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGNVEIAPFTTPDVETLLMCWLSEQQQVQWQKQGQLDFAITLAYSRRLRA
SAFVHQQGISLALRLLPLDCPCLDDLQTPAALPELLHSENGLILVTGATGSGKSTTLAAMVAYLNQHVAGHILTLEDPIE
YRHTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAPEKDPVRNQLADSLRAVLSQKLEQDKQGGRVALFELLINTPAVGNLIREGKTHQLPGIMQTGQQTGMQTFTQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=295656 AN232_RS08580 WP_110496882.1 1697268..1698248(+) (pilT) [Citrobacter sp. CRE-46 strain AR_0157]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
TTGGCGCATACGCGGAAATGTCGAAATCGCGCCGTTTACCACGCCTGACGTAGAGACGTTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCAGAAACAGGGGCAGCTGGATTTTGCCATTACGCTGGCGTACTCACGGCGTCTGCGTGCG
AGCGCATTTGTTCATCAGCAGGGAATCTCTCTGGCGCTAAGGCTATTGCCGCTTGATTGCCCATGTTTGGATGATCTCCA
GACTCCCGCCGCCTTACCTGAGCTGCTGCACAGTGAAAATGGATTAATTCTGGTGACCGGCGCTACCGGCAGCGGTAAAT
CAACGACCCTGGCGGCGATGGTGGCATATCTTAATCAGCACGTCGCGGGACATATTTTGACGCTGGAAGATCCCATTGAA
TATCGTCACACCAGCCAGCGTTGTCTCATTCAACAACGGGAGGTGGGCGTACACTGCGCTTCTTTCGCCGCCGGTTTGCG
CGGCGCGCTACGCGAAGATCCTGATGTCATTTTGCTGGGCGAGCTGCGCGACGTGGAAACCATTCGTCTGGCGTTAACGG
CTGCGGAAACCGGGCATCTGGTGCTGGCAACGTTACACACGCGGGGGGCGGCTCAGGCTATTGCGCGGCTGGTGGATACC
TTTCCTGCACCTGAGAAAGATCCGGTGCGTAATCAGCTGGCAGACAGCCTGCGGGCAGTGCTTTCGCAAAAGCTGGAACA
AGATAAGCAGGGAGGACGCGTGGCGCTATTCGAACTGCTCATCAACACGCCCGCCGTTGGTAACCTGATCCGTGAAGGGA
AAACGCATCAGCTACCCGGCATAATGCAAACCGGGCAACAGACAGGCATGCAGACGTTTACGCAAAGCCTGCAGCAGCGC
CAGGCGCAGGGACGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain Lp02

45.963

98.773

0.454
  pilT Legionella pneumophila strain ERS1305867

45.963

98.773

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.433

98.466

0.408
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

39.077

99.693

0.39
  pilU Vibrio cholerae strain A1552

40.645

95.092

0.387

Multiple sequence alignment