Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DOLE_RS15720 Genome accession   NC_009943
Coordinates   3660128..3661210 (-) Length   360 a.a.
NCBI ID   WP_012176467.1    Uniprot ID   -
Organism   Desulfosudis oleivorans Hxd3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3655128..3666210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DOLE_RS15690 - 3655670..3656026 (-) 357 WP_041280637.1 hypothetical protein -
  DOLE_RS18815 (Dole_3048) - 3656105..3656563 (-) 459 WP_083766633.1 DUF3192 domain-containing protein -
  DOLE_RS15705 (Dole_3049) - 3657325..3657837 (+) 513 WP_012176463.1 nitroreductase family protein -
  DOLE_RS18340 (Dole_3050) - 3658097..3658261 (-) 165 WP_012176464.1 hypothetical protein -
  DOLE_RS15710 (Dole_3051) - 3658258..3658932 (-) 675 WP_012176465.1 zinc-ribbon domain-containing protein -
  DOLE_RS15715 (Dole_3052) - 3658944..3660107 (-) 1164 WP_012176466.1 type IV pilus twitching motility protein PilT -
  DOLE_RS15720 (Dole_3053) pilT 3660128..3661210 (-) 1083 WP_012176467.1 type IV pilus twitching motility protein PilT Machinery gene
  DOLE_RS15725 (Dole_3054) - 3661284..3661583 (-) 300 WP_012176468.1 YggT family protein -
  DOLE_RS15730 (Dole_3055) - 3661738..3662076 (-) 339 WP_012176469.1 ferritin family protein -
  DOLE_RS15735 (Dole_3056) - 3662194..3662436 (-) 243 WP_041281289.1 DUF6868 family protein -
  DOLE_RS15740 (Dole_3057) - 3662545..3662829 (-) 285 WP_041280640.1 hypothetical protein -
  DOLE_RS15745 (Dole_3058) - 3663070..3664659 (-) 1590 WP_012176472.1 B12-binding domain-containing radical SAM protein -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 40014.09 Da        Isoelectric Point: 6.9783

>NTDB_id=29434 DOLE_RS15720 WP_012176467.1 3660128..3661210(-) (pilT) [Desulfosudis oleivorans Hxd3]
MAKIDAFFRLMHEQGASDLHLLSGLQPALRVRGDIERVKYKVLENDDLRAMLYEIAPEEKVKVFEETGDVDFGYEIQGLA
RYRVNFFMQRNGVGAVFREIPSTILNAEQLGLPPVVPRLASLPRGLVLLTGPTGSGKSTTLAAIVDEANRTRKDNIITVE
DPIEFVHESKSCIVSHREVGIHTKSFSAALRGALREDPDIILVGEMRDLETISLAIEASATGHLVFGTLHTTSAAKTVDR
IIEVFPAEQQAQIRSTLADGIRAVISQVLFKRIDKIGRCVAPEILIATPAVRNLIRESKTHQLPSMMQTGKKYGMQLLDD
AIMMLYHRGWISAEEAYNKANDKSQFRGFLKSTPKDFTEA

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=29434 DOLE_RS15720 WP_012176467.1 3660128..3661210(-) (pilT) [Desulfosudis oleivorans Hxd3]
ATGGCAAAAATTGATGCATTTTTCAGGCTGATGCACGAGCAGGGCGCGTCAGACCTTCACCTGCTCAGCGGTTTGCAGCC
CGCTCTCAGGGTTCGGGGCGATATCGAGCGGGTCAAGTACAAAGTCCTGGAAAACGACGACCTTCGGGCCATGCTTTACG
AGATCGCCCCTGAAGAAAAGGTCAAGGTGTTTGAAGAGACCGGGGACGTGGACTTCGGTTACGAGATTCAGGGCCTGGCC
CGGTACCGCGTCAATTTTTTCATGCAGAGAAACGGCGTGGGCGCCGTGTTCCGGGAGATTCCCAGCACCATTCTCAATGC
CGAGCAGCTGGGCCTGCCGCCGGTGGTGCCCAGGCTGGCCTCCCTTCCCCGGGGGCTGGTGCTGCTGACCGGCCCTACCG
GCAGCGGTAAGTCCACCACCCTGGCCGCCATCGTGGACGAGGCCAACCGGACCCGGAAAGATAACATCATCACCGTTGAA
GACCCCATTGAGTTTGTTCACGAAAGCAAGAGCTGCATCGTGAGCCACCGGGAGGTGGGCATTCACACCAAGTCCTTTTC
CGCGGCCCTTCGCGGTGCCCTTCGCGAGGACCCGGACATCATTCTGGTGGGCGAAATGCGGGACCTGGAAACCATCTCCC
TGGCCATTGAGGCGTCGGCCACCGGTCACCTGGTGTTCGGCACCCTGCATACCACCAGCGCGGCCAAGACCGTGGATCGT
ATCATTGAGGTGTTTCCGGCCGAACAGCAGGCCCAGATTCGCTCCACCCTGGCCGACGGCATTCGGGCGGTCATCTCCCA
GGTGCTGTTCAAGCGCATCGACAAGATCGGCCGGTGCGTGGCTCCGGAGATTCTCATCGCCACCCCGGCGGTGCGCAACC
TGATCCGTGAGTCCAAGACCCACCAGCTGCCCTCCATGATGCAGACCGGTAAAAAATACGGCATGCAGCTTCTGGATGAC
GCCATCATGATGCTGTATCACAGGGGGTGGATCAGCGCGGAAGAGGCATACAACAAGGCCAACGACAAGTCCCAGTTCAG
GGGCTTTCTCAAGAGCACGCCCAAGGATTTTACAGAGGCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-255)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

57.186

92.778

0.531
  pilT Pseudomonas stutzeri DSM 10701

55.988

92.778

0.519
  pilT Legionella pneumophila strain Lp02

54.683

91.944

0.503
  pilT Legionella pneumophila strain ERS1305867

54.683

91.944

0.503
  pilT Acinetobacter nosocomialis M2

53.293

92.778

0.494
  pilT Acinetobacter baumannii D1279779

53.293

92.778

0.494
  pilT Acinetobacter baumannii strain A118

53.293

92.778

0.494
  pilT Acinetobacter baylyi ADP1

52.87

91.944

0.486
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.563

95.278

0.472
  pilT Neisseria meningitidis 8013

51.988

90.833

0.472
  pilT Neisseria gonorrhoeae MS11

51.682

90.833

0.469
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

90.833

0.45
  pilT Vibrio cholerae strain A1552

49.541

90.833

0.45
  pilU Pseudomonas stutzeri DSM 10701

39.542

96.944

0.383
  pilU Vibrio cholerae strain A1552

37.853

98.333

0.372
  pilU Acinetobacter baylyi ADP1

38.304

95

0.364

Multiple sequence alignment