Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CLOS_RS08410 Genome accession   NC_009922
Coordinates   1737961..1739013 (-) Length   350 a.a.
NCBI ID   WP_012159492.1    Uniprot ID   A8MGF7
Organism   Alkaliphilus oremlandii OhILAs     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1732961..1744013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLOS_RS08385 (Clos_1632) - 1733349..1733921 (-) 573 WP_012159487.1 PilN domain-containing protein -
  CLOS_RS08390 (Clos_1633) pilM 1733924..1735198 (-) 1275 WP_012159488.1 pilus assembly protein PilM -
  CLOS_RS08395 (Clos_1634) - 1735308..1736066 (-) 759 WP_012159489.1 A24 family peptidase -
  CLOS_RS16320 (Clos_1635) - 1736116..1736511 (-) 396 WP_012159490.1 type II secretion system protein -
  CLOS_RS08405 (Clos_1636) - 1736747..1737949 (-) 1203 WP_012159491.1 type II secretion system F family protein -
  CLOS_RS08410 (Clos_1637) pilT 1737961..1739013 (-) 1053 WP_012159492.1 type IV pilus twitching motility protein PilT Machinery gene
  CLOS_RS08415 (Clos_1638) - 1739024..1740706 (-) 1683 WP_012159493.1 GspE/PulE family protein -
  CLOS_RS08420 (Clos_1639) - 1740828..1741325 (-) 498 WP_012159494.1 YqeG family HAD IIIA-type phosphatase -
  CLOS_RS08425 (Clos_1640) - 1741563..1741994 (-) 432 WP_012159495.1 hypothetical protein -
  CLOS_RS16045 - 1742338..1742514 (-) 177 WP_198006270.1 hypothetical protein -
  CLOS_RS08430 (Clos_1641) - 1742967..1743233 (-) 267 WP_012159496.1 sigma-70 family RNA polymerase sigma factor -
  CLOS_RS16325 - 1743286..1743414 (-) 129 WP_278183733.1 hypothetical protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38442.28 Da        Isoelectric Point: 8.1345

>NTDB_id=29387 CLOS_RS08410 WP_012159492.1 1737961..1739013(-) (pilT) [Alkaliphilus oremlandii OhILAs]
MNIIELVNKATIIKASDIHITVGLPPVVRINGKLENLGESKLSPNDTKNLVYATLSEKQVEVLEQNGEIDTSFSSPGAGR
YRVNAYKQRGSYGMALRIIPLEIPSAEAIGLPAVAKELARLPRGLILVTGPTGSGKSTTLASMINQINHERECHILTLED
PIEYLHKHHKSIVNQREIGTDSRSFATGLRAALRQDPDVILVGEMRDLETISIALTAAETGHLVLSTLHTNGAAKTVDRI
IDVFPPHQQQQVRIQFSSAIQAIISQQLLPKVDSSGRVAAFEVMVATPAIRNLIREDKNHQIDTAIQTGAKFQMQTMDAS
LLELYKKGIITKDTALMQAINQENIRKYII

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=29387 CLOS_RS08410 WP_012159492.1 1737961..1739013(-) (pilT) [Alkaliphilus oremlandii OhILAs]
ATGAATATAATTGAATTGGTAAATAAAGCCACCATAATCAAAGCATCAGATATTCATATCACAGTGGGATTACCTCCAGT
TGTAAGGATCAATGGGAAACTTGAAAACCTTGGAGAAAGCAAATTAAGTCCAAATGACACTAAGAATTTGGTGTACGCTA
CTTTAAGTGAAAAGCAGGTAGAGGTTTTGGAACAAAATGGAGAAATTGACACCTCTTTTTCAAGTCCGGGAGCAGGACGA
TATAGGGTGAATGCTTATAAACAAAGAGGATCCTATGGCATGGCACTGAGAATAATTCCATTAGAAATACCCAGTGCAGA
GGCAATTGGACTTCCTGCTGTCGCAAAAGAGCTTGCAAGGCTGCCGAGAGGACTGATTCTAGTTACGGGCCCTACTGGCA
GTGGAAAATCTACAACTTTAGCCTCTATGATCAATCAAATCAATCACGAAAGAGAATGTCATATATTAACTTTAGAAGAT
CCCATAGAGTATTTACATAAACATCATAAAAGTATCGTAAATCAAAGGGAGATCGGAACAGACTCAAGGTCTTTTGCTAC
AGGGCTTAGGGCAGCGTTAAGACAGGATCCAGATGTTATTTTAGTAGGAGAGATGAGAGACTTAGAAACCATATCCATAG
CACTGACCGCCGCAGAAACAGGTCATCTTGTACTATCTACTTTACACACCAATGGCGCTGCAAAAACAGTGGATCGGATC
ATTGATGTGTTTCCGCCACATCAGCAGCAGCAGGTTCGAATACAATTTTCATCTGCAATTCAAGCCATCATATCTCAGCA
GTTACTTCCAAAGGTAGATTCATCTGGAAGGGTAGCGGCTTTTGAAGTGATGGTAGCCACACCAGCCATCAGGAACCTCA
TAAGAGAGGATAAGAACCACCAGATTGATACAGCGATACAAACTGGAGCAAAATTTCAAATGCAGACAATGGACGCATCT
CTATTGGAGCTTTATAAGAAAGGAATTATAACGAAAGACACTGCATTAATGCAGGCTATCAACCAAGAAAATATTAGAAA
GTATATTATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A8MGF7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

51.17

97.714

0.5
  pilT Legionella pneumophila strain ERS1305867

51.17

97.714

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.144

99.143

0.497
  pilT Acinetobacter baumannii strain A118

49.855

98.571

0.491
  pilT Pseudomonas stutzeri DSM 10701

49.855

98.571

0.491
  pilT Acinetobacter nosocomialis M2

49.855

98.571

0.491
  pilT Acinetobacter baumannii D1279779

49.855

98.571

0.491
  pilT Pseudomonas aeruginosa PAK

49.565

98.571

0.489
  pilT Neisseria meningitidis 8013

48.986

98.571

0.483
  pilT Acinetobacter baylyi ADP1

50.448

95.714

0.483
  pilT Neisseria gonorrhoeae MS11

48.696

98.571

0.48
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.303

94.286

0.474
  pilT Vibrio cholerae strain A1552

50.303

94.286

0.474
  pilU Pseudomonas stutzeri DSM 10701

43.284

95.714

0.414
  pilU Acinetobacter baylyi ADP1

40.058

99.143

0.397
  pilU Vibrio cholerae strain A1552

40.541

95.143

0.386

Multiple sequence alignment