Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   DKK66_RS05740 Genome accession   NZ_CP029539
Coordinates   1200128..1200556 (-) Length   142 a.a.
NCBI ID   WP_131356169.1    Uniprot ID   A0A4P6TPV5
Organism   Aquitalea sp. USM4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1195128..1205556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKK66_RS05725 (DKK66_05720) rpoH 1195381..1196244 (+) 864 WP_045846375.1 RNA polymerase sigma factor RpoH -
  DKK66_RS05730 (DKK66_05725) - 1196365..1198152 (-) 1788 WP_131356163.1 PglL family O-oligosaccharyltransferase -
  DKK66_RS05735 (DKK66_05730) - 1198246..1200072 (-) 1827 WP_165494109.1 PglL family O-oligosaccharyltransferase -
  DKK66_RS05740 (DKK66_05735) pilA2 1200128..1200556 (-) 429 WP_131356169.1 pilin Machinery gene
  DKK66_RS20580 (DKK66_05740) pilA2 1200578..1201006 (-) 429 WP_131356172.1 pilin Machinery gene
  DKK66_RS05750 (DKK66_05745) - 1201195..1202544 (+) 1350 WP_131356175.1 adenosylmethionine--8-amino-7-oxononanoate transaminase -
  DKK66_RS05755 (DKK66_05750) - 1202674..1204212 (+) 1539 WP_131356177.1 hypothetical protein -
  DKK66_RS05760 (DKK66_05755) proB 1204284..1205402 (+) 1119 WP_045846370.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14727.71 Da        Isoelectric Point: 7.7772

>NTDB_id=293353 DKK66_RS05740 WP_131356169.1 1200128..1200556(-) (pilA2) [Aquitalea sp. USM4]
MNSKIQQGFTLIELMIVVAIIGILAAIAIPAYQDYTKRARVTEGLSLASAAKTSVSEYYASNNAFPDNNASAGLATTISG
TSVSDVQLSTSGVITITYKPVVDATTNQLQLTPTAGSGAVTWKCAAPATNGVPSNWLPSNCR

Nucleotide


Download         Length: 429 bp        

>NTDB_id=293353 DKK66_RS05740 WP_131356169.1 1200128..1200556(-) (pilA2) [Aquitalea sp. USM4]
ATGAACAGCAAGATCCAGCAAGGTTTTACCCTGATCGAACTGATGATCGTGGTGGCCATTATCGGTATTCTCGCCGCCAT
CGCCATCCCGGCCTATCAGGATTACACCAAGCGTGCACGTGTGACGGAAGGTCTGTCGCTGGCTTCCGCTGCCAAGACTT
CAGTTTCCGAATACTACGCATCCAACAATGCATTCCCGGACAACAATGCTTCCGCAGGTCTGGCAACCACCATTTCCGGT
ACTTCGGTGTCTGATGTACAGCTGAGTACCAGTGGTGTGATCACCATCACTTACAAGCCGGTTGTGGATGCGACTACTAA
TCAGTTGCAGCTGACCCCGACTGCCGGTTCCGGTGCGGTGACCTGGAAATGTGCTGCTCCTGCTACCAATGGTGTTCCGA
GCAACTGGCTGCCGTCCAACTGCCGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P6TPV5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

52.518

97.887

0.514
  pilA2 Legionella pneumophila strain ERS1305867

52.518

97.887

0.514
  pilA Ralstonia pseudosolanacearum GMI1000

42.262

100

0.5
  comP Acinetobacter baylyi ADP1

45.161

100

0.493
  pilE Neisseria gonorrhoeae MS11

42.073

100

0.486
  pilE Neisseria gonorrhoeae strain FA1090

42.331

100

0.486
  pilA/pilA1 Eikenella corrodens VA1

43.226

100

0.472
  pilA Vibrio campbellii strain DS40M4

43.624

100

0.458
  pilA Acinetobacter baumannii strain A118

40.426

99.296

0.401
  pilA Vibrio cholerae C6706

37.584

100

0.394
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.584

100

0.394
  pilA Vibrio cholerae strain A1552

37.584

100

0.394
  pilA Haemophilus influenzae Rd KW20

37.857

98.592

0.373
  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.054

100

0.373
  pilA Haemophilus influenzae 86-028NP

38.129

97.887

0.373

Multiple sequence alignment