Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DKM44_RS00570 Genome accession   NZ_CP029494
Coordinates   106581..107804 (-) Length   407 a.a.
NCBI ID   WP_109824539.1    Uniprot ID   A0A2Z3JKW5
Organism   Deinococcus irradiatisoli strain 17bor-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 101581..112804
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKM44_RS00545 (DKM44_00545) - 101820..102935 (-) 1116 WP_245895980.1 heparan-alpha-glucosaminide N-acetyltransferase domain-containing protein -
  DKM44_RS00550 (DKM44_00550) - 103068..103532 (-) 465 WP_245895981.1 CAP domain-containing protein -
  DKM44_RS00555 (DKM44_00555) - 103611..104789 (+) 1179 WP_109824533.1 MFS transporter -
  DKM44_RS00560 (DKM44_00560) - 104797..106095 (-) 1299 WP_146202682.1 amidase -
  DKM44_RS00565 (DKM44_00565) - 106146..106484 (-) 339 WP_109824537.1 four-helix bundle copper-binding protein -
  DKM44_RS00570 (DKM44_00570) pilT 106581..107804 (-) 1224 WP_109824539.1 type IV pilus twitching motility protein PilT Machinery gene
  DKM44_RS00575 (DKM44_00575) pilB 107864..110524 (-) 2661 WP_109824541.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  DKM44_RS00580 (DKM44_00580) - 110521..111111 (-) 591 WP_109824543.1 YqeG family HAD IIIA-type phosphatase -
  DKM44_RS00585 (DKM44_00585) pgeF 111108..111857 (-) 750 WP_245896102.1 peptidoglycan editing factor PgeF -
  DKM44_RS00590 (DKM44_00590) trmH 111995..112660 (+) 666 WP_109824547.1 tRNA (guanosine(18)-2'-O)-methyltransferase TrmH -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44415.56 Da        Isoelectric Point: 7.1470

>NTDB_id=293266 DKM44_RS00570 WP_109824539.1 106581..107804(-) (pilT) [Deinococcus irradiatisoli strain 17bor-2]
MTFGPDITDILRIAAENNASDVILTVGRPPQFKINGVYGSQDMGELSATDTRRLMYSMMNDKQQRQFEDKRELDFSFALG
EQARFRVNTFMQRGYVGGVMRLIPTKIKSVQDLGLPQTVIDIANAPRGLVLVTGPTGSGKSTTLAAMLDFINANKKLHIV
TIEDPIEFMHQHKQSIINQREVGGDTFSFDNALRAVLRQAPDVILVGEMRDYETIKAAVTAAETGHLVMGTLHTNSAPES
IDRIVDVFPEEQQAQIRVQLANNLVAVMTQQLIPRLDGGRVLAYELLVATPAVRALIREGKTYQIVSVMQTSSREGMVTM
DAFLAGLYRRRIISHDAGMERAVDAKEFARLASDPGGGNPNAVPSAGLSNSYNGSATPQNRPAAGREAVGTAYSADPRPG
GEKFGRH

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=293266 DKM44_RS00570 WP_109824539.1 106581..107804(-) (pilT) [Deinococcus irradiatisoli strain 17bor-2]
ATGACCTTTGGCCCCGACATCACCGACATTCTGCGGATCGCCGCCGAGAACAACGCCTCCGACGTCATCCTGACGGTGGG
CCGGCCGCCGCAGTTCAAGATCAACGGCGTCTACGGCAGCCAGGACATGGGCGAACTGAGCGCCACCGACACCCGGCGGC
TGATGTACAGCATGATGAACGACAAGCAGCAGCGCCAGTTCGAGGACAAGCGCGAGCTCGACTTTTCCTTCGCGCTGGGC
GAGCAGGCACGCTTCCGCGTCAACACCTTCATGCAGCGCGGCTACGTCGGCGGCGTGATGCGACTGATTCCCACCAAGAT
CAAGAGCGTGCAGGACCTGGGGTTGCCACAGACGGTCATCGACATCGCCAACGCGCCGCGCGGCCTGGTGCTGGTCACCG
GGCCCACCGGCTCGGGCAAGTCCACCACCCTGGCGGCGATGCTCGATTTCATTAACGCCAACAAGAAGCTGCACATCGTG
ACCATCGAAGACCCGATCGAGTTCATGCACCAGCACAAGCAGAGCATCATCAACCAGCGCGAGGTGGGCGGCGACACCTT
CAGCTTCGACAACGCCCTGCGCGCGGTGCTGCGCCAGGCCCCCGACGTGATTCTGGTGGGCGAAATGCGCGACTACGAGA
CCATCAAGGCCGCCGTGACCGCCGCCGAGACCGGGCACCTGGTGATGGGCACCCTGCACACCAACTCGGCGCCAGAATCC
ATCGACCGCATCGTTGACGTGTTTCCCGAAGAACAGCAGGCCCAGATCCGGGTGCAGCTGGCCAACAACCTGGTCGCGGT
GATGACCCAGCAGCTCATTCCGCGCCTCGACGGCGGCCGGGTGCTGGCCTACGAACTACTGGTCGCCACCCCGGCGGTGC
GCGCGCTGATCCGCGAGGGCAAGACCTACCAGATCGTCTCGGTGATGCAGACCAGTTCGCGCGAGGGCATGGTCACCATG
GACGCCTTCCTGGCAGGACTCTACCGCCGACGCATCATCAGCCACGACGCGGGCATGGAGCGGGCGGTGGACGCCAAGGA
ATTCGCCCGGCTGGCCAGCGATCCCGGCGGCGGCAACCCCAACGCGGTGCCCAGCGCGGGCCTGAGCAATTCCTACAACG
GCTCGGCCACGCCGCAAAACCGCCCGGCGGCCGGCCGGGAAGCGGTGGGCACCGCCTACAGCGCCGACCCCCGGCCCGGC
GGCGAGAAATTCGGCCGGCACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z3JKW5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

76.053

93.366

0.71
  pilT Acinetobacter nosocomialis M2

53.799

80.835

0.435
  pilT Acinetobacter baumannii D1279779

53.799

80.835

0.435
  pilT Acinetobacter baumannii strain A118

53.799

80.835

0.435
  pilT Neisseria meningitidis 8013

50.725

84.767

0.43
  pilT Pseudomonas stutzeri DSM 10701

50.729

84.275

0.428
  pilT Neisseria gonorrhoeae MS11

50.291

84.521

0.425
  pilT Pseudomonas aeruginosa PAK

51.039

82.801

0.423
  pilT Acinetobacter baylyi ADP1

51.672

80.835

0.418
  pilT Legionella pneumophila strain Lp02

48.688

84.275

0.41
  pilT Legionella pneumophila strain ERS1305867

48.688

84.275

0.41
  pilT Vibrio cholerae strain A1552

50.61

80.59

0.408
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.61

80.59

0.408
  pilU Pseudomonas stutzeri DSM 10701

42.308

89.435

0.378
  pilU Acinetobacter baylyi ADP1

43.658

83.292

0.364

Multiple sequence alignment