Detailed information NTG0293267

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: DKM44_RS00575
• Genome accession: NZ_CP029494
• Coordinates: 107864..110524 (-)
• Length: 886 a.a.
• NCBI ID: WP_109824541.1
• Uniprot ID: A0A2Z3JAA3
• Organism: Deinococcus irradiatisoli strain 17bor-2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 102864..115524

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DKM44_RS00550 (DKM44_00550)	-	103068..103532 (-)	465	WP_245895981.1	CAP domain-containing protein	-
DKM44_RS00555 (DKM44_00555)	-	103611..104789 (+)	1179	WP_109824533.1	MFS transporter	-
DKM44_RS00560 (DKM44_00560)	-	104797..106095 (-)	1299	WP_146202682.1	amidase	-
DKM44_RS00565 (DKM44_00565)	-	106146..106484 (-)	339	WP_109824537.1	four-helix bundle copper-binding protein	-
DKM44_RS00570 (DKM44_00570)	pilT	106581..107804 (-)	1224	WP_109824539.1	type IV pilus twitching motility protein PilT	Machinery gene
DKM44_RS00575 (DKM44_00575)	pilB	107864..110524 (-)	2661	WP_109824541.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
DKM44_RS00580 (DKM44_00580)	-	110521..111111 (-)	591	WP_109824543.1	YqeG family HAD IIIA-type phosphatase	-
DKM44_RS00585 (DKM44_00585)	pgeF	111108..111857 (-)	750	WP_245896102.1	peptidoglycan editing factor PgeF	-
DKM44_RS00590 (DKM44_00590)	trmH	111995..112660 (+)	666	WP_109824547.1	tRNA (guanosine(18)-2'-O)-methyltransferase TrmH	-
DKM44_RS00595 (DKM44_00595)	-	112856..113431 (+)	576	WP_109824549.1	TerD family protein	-
DKM44_RS00600 (DKM44_00600)	-	113540..114799 (+)	1260	WP_109824551.1	TerD family protein	-

Sequence

Protein

• Length: 886 a.a.
• Molecular weight: 96865.96 Da
• Isoelectric Point: 5.1201

>NTDB_id=293267 DKM44_RS00575 WP_109824541.1 107864..110524(-) (pilB) [Deinococcus irradiatisoli strain 17bor-2]
MTLSIGDRRLGAILLEQGYVNDADLQRALDRHLEVGGRLADILIDGGLVGEKRIARALEEAFGLPLVDLTALTPDPQAIA
SIPASAAVQAQGVPFALENGSLRVAFVDALNNVALETMEDESGYIIEPYQALREQVNWAIASFYPELGLPIPTLGGETQG
VSLLGQRLVARGFVSEAQIMNALEQQRHSGVSLGTVLLGMKLITEEQLYETLAEQGGAQYVRDTREYRPGEEVLGLLLRA
DALRLAAVPVSQEGGVVSIITGDLRRREDIEALIGREVRLLLARPSDVDNLIERAYPQKGRLGEAMVQQGTLSRAQLREA
LQVQARQGKVKPLGEVIVELGFAASDEIDAALVKQNAGGGRLEDTLVQSGKLSPEMLARSLAAQLGYEFVDPVTSPPDTK
VALMIPEATARRYTVVPMRLQGESLVIAMKDPRNVFALDDLKLITGREILPAVMAEKDIIRLIERYFGDKDMAKLNEELA
KTSRQKEKEQETVDLSSLDDNAVVRVVDGLIREAALQDASDIHIEPTEHSVRVRYRIDGSLREHMELPRGAAQSMLARIK
IMGNLDISERRVPQDGRVRFKKGSIDLDLRLSTLPTVYGEKAVMRLLQKASNIPEVEQLGFSEHNFGRFLDVIEKPNGIF
LVTGPTGSGKSFTSFSTLKRIARPDKNTTTIEDPIEYEIPGIIQSQVNTTAGLTFARALRAFLRQDPDIIFVGEIRDTET
AKIATEAALTGHLVLATLHTNDAPGAITRLDEMGVEPFNISASVIGVLAQRLVRRVCTECKRPTNADPEVLRRLGIGERE
LRGANLQRGAGCPRCGGTGYKGRMGIHELMVVDDPIRRAIGQRQTAAEIREIAVEQSHMKTLRQDGIEKALVGTTTLEEV
LAVTSS

Nucleotide

• Length: 2661 bp

>NTDB_id=293267 DKM44_RS00575 WP_109824541.1 107864..110524(-) (pilB) [Deinococcus irradiatisoli strain 17bor-2]
GTGACACTTTCGATTGGAGACCGCCGCCTCGGCGCCATCTTGCTGGAGCAGGGCTACGTCAACGACGCCGACCTGCAGCG
TGCCCTCGACCGCCACCTGGAAGTGGGCGGCCGCCTGGCCGACATCCTGATCGACGGCGGTCTGGTGGGCGAGAAGCGCA
TCGCCCGCGCCCTGGAAGAAGCCTTCGGCCTGCCGCTGGTCGACCTCACCGCCCTGACGCCCGATCCGCAGGCCATCGCC
AGCATTCCGGCCTCGGCGGCGGTACAGGCCCAGGGCGTGCCGTTCGCGCTGGAAAACGGCAGCCTGCGGGTGGCCTTCGT
GGACGCCCTCAACAACGTGGCGCTCGAAACCATGGAAGACGAGAGCGGCTACATCATCGAGCCGTACCAGGCGCTGCGCG
AGCAGGTGAACTGGGCCATCGCCAGCTTCTACCCGGAACTCGGCCTGCCGATTCCCACGCTGGGCGGCGAAACGCAGGGG
GTCTCGCTGCTGGGCCAGCGCCTGGTGGCGCGCGGTTTCGTCAGCGAGGCGCAGATCATGAACGCGCTGGAGCAGCAGCG
CCACAGCGGAGTCTCGCTCGGCACGGTGCTGCTGGGCATGAAGCTGATCACCGAAGAGCAGCTCTACGAGACGCTGGCCG
AGCAGGGCGGCGCCCAGTACGTGCGCGATACCCGCGAGTACCGCCCCGGCGAGGAAGTGCTGGGGTTGCTGCTGCGCGCC
GACGCGCTGCGCCTGGCGGCGGTGCCGGTGAGCCAGGAAGGCGGCGTGGTCAGCATCATCACCGGCGACCTGCGCCGGCG
CGAGGACATCGAGGCGCTGATCGGCCGCGAGGTCCGGCTGCTGCTGGCGCGGCCCAGCGACGTGGACAACCTGATCGAGC
GCGCTTACCCGCAAAAGGGTCGGCTGGGCGAGGCGATGGTGCAGCAGGGCACCCTGTCGCGCGCCCAGCTGCGCGAAGCC
CTGCAGGTGCAGGCCCGCCAGGGCAAGGTCAAGCCGCTGGGCGAGGTGATCGTCGAGTTGGGCTTTGCCGCCAGCGACGA
GATCGACGCCGCGCTGGTCAAGCAGAATGCCGGCGGCGGGCGCCTGGAAGACACCCTGGTGCAGTCCGGCAAGCTCAGCC
CCGAGATGCTGGCGCGCTCGCTGGCCGCGCAGCTCGGTTACGAATTCGTCGATCCGGTGACCTCGCCGCCCGACACCAAG
GTGGCCCTAATGATTCCCGAGGCCACCGCCCGGCGCTACACCGTGGTGCCGATGAGGTTGCAGGGCGAGTCGCTGGTCAT
CGCCATGAAAGACCCGCGCAACGTGTTTGCGCTCGACGACCTCAAGCTGATCACCGGGCGCGAGATCCTGCCGGCGGTGA
TGGCCGAAAAAGACATCATCCGCCTGATCGAGCGCTACTTCGGCGACAAGGACATGGCCAAGCTCAACGAGGAGCTCGCC
AAGACCAGCCGCCAGAAGGAAAAAGAGCAGGAAACGGTCGACCTCTCCTCGCTCGACGACAACGCGGTGGTGCGGGTGGT
GGACGGCCTGATCCGCGAGGCCGCCCTGCAGGACGCCTCGGACATTCACATCGAGCCCACCGAGCACAGCGTGCGGGTGC
GCTACCGCATCGACGGTTCGCTGCGCGAGCACATGGAACTGCCCAGGGGCGCGGCCCAGAGCATGCTGGCGCGCATCAAG
ATCATGGGCAACCTCGACATCTCCGAGCGCCGCGTGCCGCAGGACGGCCGGGTGCGGTTCAAGAAAGGCAGCATCGACCT
CGACCTGCGCCTCTCGACCCTGCCCACCGTCTACGGCGAGAAGGCCGTGATGCGTCTGTTGCAAAAAGCCTCCAACATTC
CCGAAGTCGAGCAGCTGGGCTTTTCCGAGCACAACTTCGGGCGCTTTCTGGACGTGATCGAAAAGCCCAACGGCATCTTC
CTGGTCACCGGCCCCACCGGTTCGGGCAAGTCGTTTACCAGTTTCTCGACTTTGAAGCGCATCGCCCGGCCCGACAAGAA
CACCACCACCATCGAAGACCCTATCGAATACGAGATTCCGGGCATCATTCAGTCGCAGGTCAACACCACCGCCGGGCTGA
CCTTCGCCCGGGCGCTGCGGGCCTTTTTGCGCCAGGACCCCGACATCATCTTCGTCGGCGAGATTCGCGACACCGAGACC
GCCAAGATCGCCACCGAGGCGGCCCTCACCGGCCACCTGGTGCTGGCGACCCTGCACACCAACGACGCGCCCGGCGCGAT
CACCCGCCTCGACGAGATGGGCGTGGAGCCGTTCAACATCTCGGCCTCGGTGATCGGGGTGCTGGCGCAGCGCCTGGTGC
GCCGGGTGTGCACCGAGTGCAAGCGCCCCACCAACGCCGACCCCGAGGTGCTGCGGCGCCTGGGCATCGGCGAGCGCGAA
CTGCGCGGCGCGAACCTGCAGCGCGGCGCCGGTTGCCCGCGCTGCGGCGGCACCGGCTACAAGGGCCGCATGGGCATTCA
CGAACTGATGGTGGTGGACGACCCGATTCGCCGCGCCATCGGGCAGCGCCAGACCGCCGCCGAGATCCGCGAGATCGCCG
TGGAGCAGAGCCACATGAAAACGCTGCGCCAGGATGGCATCGAAAAAGCCCTGGTCGGCACCACCACCCTCGAAGAAGTA
CTGGCCGTGACCAGCAGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (511-775)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (198-300)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-145)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (366-470)

3D structure

• AlphaFold DB: A0A2Z3JAA3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	72.441	100	0.727
pilF	Thermus thermophilus HB27	57.015	100	0.573