Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DJ564_RS30675 Genome accession   NZ_CP029482
Coordinates   6479954..6480988 (-) Length   344 a.a.
NCBI ID   WP_109635591.1    Uniprot ID   A0A2U8U0L7
Organism   Pseudomonas sp. 31-12     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6474954..6485988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DJ564_RS30645 (DJ564_30645) ruvX 6475086..6475523 (+) 438 WP_005792259.1 Holliday junction resolvase RuvX -
  DJ564_RS30650 (DJ564_30650) pyrR 6475615..6476118 (+) 504 WP_109635584.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  DJ564_RS30655 (DJ564_30655) - 6476146..6477150 (+) 1005 WP_007897943.1 aspartate carbamoyltransferase catalytic subunit -
  DJ564_RS30660 (DJ564_30660) - 6477147..6478418 (+) 1272 WP_109635586.1 dihydroorotase -
  DJ564_RS30665 (DJ564_30665) - 6478513..6478944 (-) 432 WP_109635587.1 TM2 domain-containing protein -
  DJ564_RS30670 (DJ564_30670) - 6479194..6479811 (+) 618 WP_109635589.1 C40 family peptidase -
  DJ564_RS30675 (DJ564_30675) pilT 6479954..6480988 (-) 1035 WP_109635591.1 type IV pilus twitching motility protein PilT Machinery gene
  DJ564_RS30680 (DJ564_30680) - 6481045..6481731 (+) 687 WP_109635593.1 YggS family pyridoxal phosphate-dependent enzyme -
  DJ564_RS30685 (DJ564_30685) proC 6481767..6482585 (+) 819 WP_109635595.1 pyrroline-5-carboxylate reductase -
  DJ564_RS30690 (DJ564_30690) - 6482610..6483197 (+) 588 WP_010466835.1 YggT family protein -
  DJ564_RS30695 (DJ564_30695) - 6483197..6483493 (+) 297 WP_109635596.1 DUF167 domain-containing protein -
  DJ564_RS30700 (DJ564_30700) - 6483780..6484919 (+) 1140 WP_109635598.1 homoserine O-acetyltransferase -
  DJ564_RS30705 (DJ564_30705) metW 6484927..6485547 (+) 621 WP_010466828.1 methionine biosynthesis protein MetW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38127.76 Da        Isoelectric Point: 7.0158

>NTDB_id=293102 DJ564_RS30675 WP_109635591.1 6479954..6480988(-) (pilT) [Pseudomonas sp. 31-12]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMDDLGMGEVFRKITEAPRGLVLVTGPTGSGKSTTLAAMIDYLNNHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRIAAHEIMLGTSAIRNLIREDKVAQMYSSIQTGGNLGMQTLDMCL
KDLVTKGLISREHAREKARSPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=293102 DJ564_RS30675 WP_109635591.1 6479954..6480988(-) (pilT) [Pseudomonas sp. 31-12]
ATGGATATCACCGAGCTGCTGGCCTTCAGCGCTAAACAGGGCGCATCCGACCTGCACCTGTCTGCCGGACTGCCACCGAT
GATTCGCGTTGACGGCGATGTGCGGCGCATCAACCTGCCGGCCCTGGACCACAAACAGGTGCACGAGCTGATCTACGACA
TCATGAACGACACCCAGCGGGTGGACTTCGAGAAACACCTGGAAACTGATTTTTCCTTCGAAGTGCCCGGCGTGGCGCGT
TTCCGGGTCAATGCCTTCAACCAGAACCGGGGGGCGGGCGCGGTGTTCCGGACCATCCCGTCGAAGGTCCTGAGCATGGA
CGACCTCGGCATGGGCGAGGTGTTTCGCAAGATCACCGAGGCTCCGCGCGGCCTGGTGCTGGTGACCGGCCCCACCGGTT
CCGGCAAGTCCACCACCCTGGCGGCGATGATCGATTACCTGAACAACCATCGCCATCACCACATCCTCACCATCGAAGAC
CCGATCGAGTTCGTCCACGAATCGCGCAAATGCCTGATCAATCAGCGCGAAGTCCATCGCGACACCCGCAGCTTCGCCAC
GGCCCTGCGCTCGGCGCTGCGGGAAGACCCGGACGTGATTCTGGTGGGGGAGATGCGTGATCTGGAGACCATTCGCCTGG
CGCTGACCGCCGCCGAGACTGGCCATTTGGTATTCGGCACGCTGCACACCACATCGGCGGCCAAGACCATCGACCGGGTG
GTGGACGTGTTTCCGGGGGACGAGAAGTCGATGGTGCGTTCGATGCTGTCCGAGTCGTTACTGGCGGTGGTGTCGCAGAC
CTTGATCAAGAAGATCGGTGGCGGGCGGATTGCGGCGCACGAAATCATGCTCGGCACGTCGGCAATCCGTAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTACTCGTCGATTCAGACCGGGGGCAATCTGGGGATGCAGACGCTGGATATGTGCTTG
AAGGACTTGGTGACCAAGGGCTTGATCAGCCGGGAGCATGCGCGGGAGAAGGCGCGGTCGCCGGATAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U8U0L7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

87.791

100

0.878
  pilT Pseudomonas aeruginosa PAK

87.209

100

0.872
  pilT Acinetobacter nosocomialis M2

79.07

100

0.791
  pilT Acinetobacter baumannii D1279779

79.07

100

0.791
  pilT Acinetobacter baumannii strain A118

79.07

100

0.791
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Legionella pneumophila strain Lp02

71.512

100

0.715
  pilT Legionella pneumophila strain ERS1305867

71.512

100

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.965

0.669
  pilT Vibrio cholerae strain A1552

68.249

97.965

0.669
  pilT Neisseria meningitidis 8013

66.087

100

0.663
  pilT Neisseria gonorrhoeae MS11

65.797

100

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Acinetobacter baylyi ADP1

41.04

100

0.413
  pilU Pseudomonas stutzeri DSM 10701

40.29

100

0.404
  pilU Vibrio cholerae strain A1552

40

100

0.401

Multiple sequence alignment