Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DFH04_RS08445 Genome accession   NZ_CP029458
Coordinates   1787034..1788089 (+) Length   351 a.a.
NCBI ID   WP_120362037.1    Uniprot ID   -
Organism   Clostridium novyi strain 150557     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1782034..1793089
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DFH04_RS08425 (DFH04_08425) - 1782568..1783794 (+) 1227 WP_120362035.1 peptidase U32 family protein -
  DFH04_RS08430 (DFH04_08430) udk 1783798..1784424 (+) 627 WP_003376499.1 uridine kinase -
  DFH04_RS08435 (DFH04_08435) - 1784485..1786158 (+) 1674 WP_120362036.1 penicillin-binding transpeptidase domain-containing protein -
  DFH04_RS08440 (DFH04_08440) sigK 1786225..1786932 (+) 708 WP_003376105.1 RNA polymerase sporulation sigma factor SigK -
  DFH04_RS08445 (DFH04_08445) pilT 1787034..1788089 (+) 1056 WP_120362037.1 type IV pilus twitching motility protein PilT Machinery gene
  DFH04_RS08450 (DFH04_08450) ftsA 1788342..1789598 (+) 1257 WP_003375014.1 cell division protein FtsA -
  DFH04_RS08455 (DFH04_08455) ftsZ 1789614..1790792 (+) 1179 WP_120362038.1 cell division protein FtsZ -
  DFH04_RS08460 (DFH04_08460) spoIIGA 1790971..1791756 (+) 786 WP_039234228.1 sigma-E processing peptidase SpoIIGA -
  DFH04_RS08465 (DFH04_08465) sigE 1791770..1792477 (+) 708 WP_003376436.1 RNA polymerase sporulation sigma factor SigE -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39046.25 Da        Isoelectric Point: 9.6061

>NTDB_id=292527 DFH04_RS08445 WP_120362037.1 1787034..1788089(+) (pilT) [Clostridium novyi strain 150557]
MIPLNELLKKTIDQNASDLHLTVGISPVIRINGELIRIGEQKLTANDTRKYVIEILGDSFKKYDNIGEIDTSISIPSVGR
FRVNIYKQRGSYALAIRAVSLKIPSISQLKLPSIIKDISDKRRGLILVTGPTGSGKSTTLASIINEINNKRSAHIITLED
PIEFLHKHNKSIINQREIGNDTSSYTDALRAALREDPDVILVGEMRDLETMATAITAAETGHLVLSTLHTIGAAKTIDRI
IDVFPPHQQQQIKIQLSTVLQGVISQQLIPKYDGKGQIASFEIMVLNSAIQNLIREGKTHQIQSLIQTGSKYGMKTMDMS
IVELYKDKLISYENALTYSIDKSMVKRMIDM

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=292527 DFH04_RS08445 WP_120362037.1 1787034..1788089(+) (pilT) [Clostridium novyi strain 150557]
GTGATACCATTAAATGAGCTATTAAAAAAGACCATAGATCAGAATGCATCTGATTTACATTTAACTGTTGGAATTTCTCC
GGTAATTAGAATAAATGGTGAACTAATAAGAATTGGCGAACAAAAACTTACGGCAAATGATACAAGAAAGTATGTTATAG
AAATATTAGGAGACTCATTTAAAAAATATGACAATATTGGTGAAATAGATACATCTATATCTATTCCCAGTGTAGGAAGG
TTTAGAGTTAATATATATAAACAGAGAGGGAGTTATGCCCTTGCTATAAGAGCAGTTTCATTAAAAATACCTTCTATTAG
TCAATTAAAGTTACCATCTATAATAAAAGATATAAGTGACAAACGTAGAGGACTTATTCTTGTAACAGGCCCAACTGGAA
GTGGAAAAAGTACTACTTTAGCATCTATAATAAATGAGATAAATAATAAAAGATCAGCTCATATTATTACATTAGAAGAT
CCTATAGAATTTCTTCATAAGCATAACAAATCAATAATTAATCAAAGGGAAATAGGAAATGATACATCATCTTACACCGA
TGCATTAAGAGCAGCACTTAGAGAAGATCCAGATGTAATTCTTGTTGGTGAGATGAGAGATCTTGAAACTATGGCAACGG
CAATTACAGCGGCAGAAACAGGACATTTAGTATTATCAACTTTACATACTATAGGGGCAGCTAAAACTATAGATAGGATA
ATAGATGTTTTCCCTCCACATCAGCAACAACAAATAAAAATACAACTTTCAACTGTACTTCAAGGTGTTATATCTCAACA
ACTAATACCAAAGTATGACGGAAAAGGACAAATAGCTTCTTTTGAAATTATGGTACTAAATTCTGCTATACAAAATCTTA
TAAGAGAAGGAAAAACTCATCAAATTCAATCATTAATTCAAACAGGAAGTAAGTATGGTATGAAAACCATGGATATGAGT
ATAGTTGAATTATATAAAGATAAATTAATATCATATGAAAATGCTTTAACGTATTCTATTGATAAAAGTATGGTAAAAAG
AATGATAGATATGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

50.751

94.872

0.481
  pilT Acinetobacter baumannii strain A118

49.852

96.011

0.479
  pilT Acinetobacter nosocomialis M2

49.852

96.011

0.479
  pilT Acinetobacter baumannii D1279779

49.852

96.011

0.479
  pilT Neisseria gonorrhoeae MS11

50.45

94.872

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.429

99.715

0.473
  pilT Acinetobacter baylyi ADP1

49.55

94.872

0.47
  pilT Pseudomonas aeruginosa PAK

49.249

94.872

0.467
  pilT Legionella pneumophila strain Lp02

48.235

96.866

0.467
  pilT Legionella pneumophila strain ERS1305867

48.235

96.866

0.467
  pilT Vibrio cholerae strain A1552

50.313

91.168

0.459
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.313

91.168

0.459
  pilT Pseudomonas stutzeri DSM 10701

47.447

94.872

0.45
  pilU Acinetobacter baylyi ADP1

40.708

96.581

0.393
  pilU Vibrio cholerae strain A1552

39.359

97.721

0.385
  pilU Pseudomonas stutzeri DSM 10701

38.395

99.43

0.382

Multiple sequence alignment