Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DKC05_RS18540 Genome accession   NZ_CP029449
Coordinates   3476007..3477029 (+) Length   340 a.a.
NCBI ID   WP_047729853.1    Uniprot ID   A0AB33FSA6
Organism   Serratia marcescens strain CAV1761     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3471007..3482029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKC05_RS28885 (DKC05_18815) - 3471699..3471782 (+) 84 Protein_3233 DUF2442 domain-containing protein -
  DKC05_RS18510 (DKC05_18820) hemW 3471819..3472961 (-) 1143 WP_047729847.1 radical SAM family heme chaperone HemW -
  DKC05_RS18515 (DKC05_18825) - 3472954..3473547 (-) 594 WP_047729848.1 XTP/dITP diphosphatase -
  DKC05_RS18520 (DKC05_18830) yggU 3473565..3473855 (-) 291 WP_047729849.1 DUF167 family protein YggU -
  DKC05_RS18525 (DKC05_18835) - 3473852..3474406 (-) 555 WP_047729850.1 YggT family protein -
  DKC05_RS18530 (DKC05_18840) proC 3474438..3475259 (-) 822 WP_047729851.1 pyrroline-5-carboxylate reductase -
  DKC05_RS18535 (DKC05_18845) - 3475279..3475986 (-) 708 WP_047729852.1 YggS family pyridoxal phosphate-dependent enzyme -
  DKC05_RS18540 (DKC05_18850) pilT 3476007..3477029 (+) 1023 WP_047729853.1 type IV pilus twitching motility protein PilT Machinery gene
  DKC05_RS18545 (DKC05_18855) ruvX 3477026..3477448 (-) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  DKC05_RS18550 (DKC05_18860) - 3477448..3478011 (-) 564 WP_033635940.1 YqgE/AlgH family protein -
  DKC05_RS18555 (DKC05_18865) gshB 3478157..3479107 (-) 951 WP_047729854.1 glutathione synthase -
  DKC05_RS18560 (DKC05_18870) rsmE 3479118..3479849 (-) 732 WP_033635938.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DKC05_RS18565 (DKC05_18875) endA 3479919..3480614 (-) 696 WP_047729855.1 deoxyribonuclease I -
  DKC05_RS18570 (DKC05_18880) - 3480716..3481228 (-) 513 WP_016930082.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36401.67 Da        Isoelectric Point: 7.0670

>NTDB_id=292454 DKC05_RS18540 WP_047729853.1 3476007..3477029(+) (pilT) [Serratia marcescens strain CAV1761]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAASTLTAQGVQALCDGLLSAQQRESLRRLGQIDLALHRPGGE
RLRANVFQQSAGMSLALRRIAGQAPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDTALRAALREDPDVILLGELRDVATIRLAFTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCVGEGAGS

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=292454 DKC05_RS18540 WP_047729853.1 3476007..3477029(+) (pilT) [Serratia marcescens strain CAV1761]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGTCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAGTGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGATCGATCTGGCGCTGCACAGGCCGGGTGGGGAG
CGGCTGCGAGCCAACGTTTTTCAGCAAAGCGCGGGGATGTCTCTCGCATTGCGGCGCATCGCCGGGCAGGCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGACGACGGGCTTCTTCTGGTCACCGGCGCCACCG
GCAGCGGCAAATCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGTAGCCGACGTTCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGTCACAGCTTCGA
TACGGCGCTGCGCGCTGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATGTTGCCACCATTCGGC
TGGCGTTCACCGCGGCGGAGACTGGCCATCTGGTGCTGGCGACGTTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGATGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGCGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGC
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGATTGTGTTGGCGAAGGGGCGGGGAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(43-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.636

97.059

0.521
  pilT Vibrio cholerae strain A1552

53.636

97.059

0.521
  pilT Acinetobacter baylyi ADP1

50.453

97.353

0.491
  pilT Neisseria meningitidis 8013

49.254

98.529

0.485
  pilT Acinetobacter nosocomialis M2

50

97.059

0.485
  pilT Acinetobacter baumannii D1279779

50

97.059

0.485
  pilT Acinetobacter baumannii strain A118

50

97.059

0.485
  pilT Neisseria gonorrhoeae MS11

49.245

97.353

0.479
  pilT Pseudomonas aeruginosa PAK

48.788

97.059

0.474
  pilT Legionella pneumophila strain ERS1305867

48.64

97.353

0.474
  pilT Legionella pneumophila strain Lp02

48.64

97.353

0.474
  pilT Pseudomonas stutzeri DSM 10701

48.182

97.059

0.468
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.38

100

0.453
  pilU Vibrio cholerae strain A1552

39.877

95.882

0.382
  pilU Acinetobacter baylyi ADP1

39.628

95

0.376
  pilU Pseudomonas stutzeri DSM 10701

38.272

95.294

0.365

Multiple sequence alignment