Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DGM85_RS04040 Genome accession   NZ_CP029280
Coordinates   934257..935390 (-) Length   377 a.a.
NCBI ID   WP_017157249.1    Uniprot ID   A0A3T0G2E0
Organism   Xanthomonas phaseoli pv. phaseoli strain Px100     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 929257..940390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DGM85_RS04015 (DGM85_04210) - 929346..929681 (-) 336 WP_017157246.1 phasin family protein -
  DGM85_RS04020 (DGM85_04215) queD 929875..930228 (-) 354 WP_008576903.1 6-carboxytetrahydropterin synthase QueD -
  DGM85_RS04025 (DGM85_04220) - 930364..931752 (-) 1389 WP_039571696.1 DEAD/DEAH box helicase -
  DGM85_RS04030 (DGM85_04230) - 932061..933119 (+) 1059 WP_180301647.1 fumarylacetoacetate hydrolase family protein -
  DGM85_RS04035 (DGM85_04235) maiA 933151..933813 (+) 663 WP_039571699.1 maleylacetoacetate isomerase -
  DGM85_RS04040 (DGM85_04240) pilU 934257..935390 (-) 1134 WP_017157249.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DGM85_RS04045 (DGM85_04245) - 935489..935872 (+) 384 WP_039571704.1 DUF4398 domain-containing protein -
  DGM85_RS04050 (DGM85_04250) - 935869..936759 (+) 891 WP_041019045.1 membrane protein -
  DGM85_RS04055 (DGM85_04255) - 937110..937328 (+) 219 WP_003484103.1 YdcH family protein -
  DGM85_RS04060 (DGM85_04260) - 937565..938935 (+) 1371 WP_215825358.1 pyridoxal-phosphate dependent enzyme -
  DGM85_RS04065 (DGM85_04265) - 939035..940222 (+) 1188 WP_039571715.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41720.09 Da        Isoelectric Point: 6.1401

>NTDB_id=291583 DGM85_RS04040 WP_017157249.1 934257..935390(-) (pilU) [Xanthomonas phaseoli pv. phaseoli strain Px100]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKGVDGRRRPATEVLLNTPMIRDLLRRGQVHEVKAAMEESLEEGMQTFDQCL
FRMVKTGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYEAAAAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=291583 DGM85_RS04040 WP_017157249.1 934257..935390(-) (pilU) [Xanthomonas phaseoli pv. phaseoli strain Px100]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAGGGCAAGCTTTACCCGCTCGGCAATACGGGGTTGCCGCCGGGGATGGTCAAGAAAATCGCCTACTCAT
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTCGAGCTCAACATGGCCATCGCCCTGCCAGACGCCGGCCGC
TTCCGCGTGAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTGATTCGCGCCATTCGCAGCCGGATCCCGAGCATCGA
AGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGTTCGACCGGTT
CGGGCAAGTCGACCTCACTGGCCTCGATGATCGACCACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATACCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTCGGCCTGGACACCCACGCCTTCCAGAG
CGCGCTGAAGAACGCCATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCCGAAACCATGGAGGCCG
CCATTGCGTTCGCCGAAACCGGCCACCTGTGCCTGGCGACGCTGCACTCCAACAATGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCGGTCATTTCCCAACG
TCTGGTCAAGGGCGTGGACGGCCGTCGCCGACCGGCCACCGAAGTGCTGCTCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGGTCAAGGCAGCGATGGAAGAATCGCTGGAAGAAGGCATGCAGACCTTCGACCAATGCCTG
TTCCGGATGGTCAAGACCGGCGAGATCGAGCAGGAAGAAGCGTTGCGCGCGGCCGATTCGCGTGACGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCGGCTCCGGCGAACACGACCCATACGCGGACTACGAAGCGGCCGCGGCACCGAAGATCAGTC
ACGGGTTCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3T0G2E0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

95.225

0.496
  pilU Vibrio cholerae strain A1552

49.722

95.491

0.475
  pilU Acinetobacter baylyi ADP1

49.718

93.899

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.241

100

0.448
  pilT Pseudomonas aeruginosa PAK

43.195

89.655

0.387
  pilT Acinetobacter baumannii D1279779

41.716

89.655

0.374
  pilT Acinetobacter nosocomialis M2

41.716

89.655

0.374
  pilT Acinetobacter baumannii strain A118

41.716

89.655

0.374
  pilT Pseudomonas stutzeri DSM 10701

41.916

88.594

0.371
  pilT Acinetobacter baylyi ADP1

41.176

90.186

0.371
  pilT Legionella pneumophila strain Lp02

41.124

89.655

0.369
  pilT Legionella pneumophila strain ERS1305867

41.124

89.655

0.369
  pilT Vibrio cholerae strain A1552

41.441

88.329

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

88.329

0.366
  pilT Neisseria gonorrhoeae MS11

39.481

92.042

0.363
  pilT Neisseria meningitidis 8013

39.481

92.042

0.363

Multiple sequence alignment