Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AM377_RS21120 Genome accession   NZ_CP028947
Coordinates   4389047..4390069 (-) Length   340 a.a.
NCBI ID   WP_108681987.1    Uniprot ID   -
Organism   Serratia marcescens strain AR_0130     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4384047..4395069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM377_RS21090 (AM377_21090) - 4384846..4385358 (+) 513 WP_047026451.1 SprT family zinc-dependent metalloprotease -
  AM377_RS21095 (AM377_21095) endA 4385460..4386155 (+) 696 WP_033644681.1 deoxyribonuclease I -
  AM377_RS21100 (AM377_21100) rsmE 4386225..4386956 (+) 732 WP_025304244.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AM377_RS21105 (AM377_21105) gshB 4386967..4387917 (+) 951 WP_033651325.1 glutathione synthase -
  AM377_RS21110 (AM377_21110) - 4388065..4388628 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  AM377_RS21115 (AM377_21115) ruvX 4388628..4389050 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  AM377_RS21120 (AM377_21120) pilT 4389047..4390069 (-) 1023 WP_108681987.1 type IV pilus twitching motility protein PilT Machinery gene
  AM377_RS21125 (AM377_21125) - 4390090..4390797 (+) 708 WP_042785369.1 YggS family pyridoxal phosphate-dependent enzyme -
  AM377_RS21130 (AM377_21130) proC 4390817..4391638 (+) 822 WP_033644672.1 pyrroline-5-carboxylate reductase -
  AM377_RS21135 (AM377_21135) - 4391670..4392224 (+) 555 WP_042785370.1 YggT family protein -
  AM377_RS21140 (AM377_21140) yggU 4392221..4392511 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  AM377_RS21145 (AM377_21145) - 4392529..4393122 (+) 594 WP_108681989.1 XTP/dITP diphosphatase -
  AM377_RS21150 (AM377_21150) hemW 4393115..4394257 (+) 1143 WP_089192250.1 radical SAM family heme chaperone HemW -
  AM377_RS21155 (AM377_21155) - 4394294..4394542 (-) 249 WP_025304253.1 DUF2442 domain-containing protein -
  AM377_RS21160 (AM377_21160) - 4394526..4394768 (-) 243 WP_004937418.1 DUF4160 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36408.64 Da        Isoelectric Point: 7.2888

>NTDB_id=288799 AM377_RS21120 WP_108681987.1 4389047..4390069(-) (pilT) [Serratia marcescens strain AR_0130]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAQTLTAQGAQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANVFQQSAGISLALRRIAGQSPSLAELAAPAIVPALLRRADGLILITGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLTTLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCAGEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=288799 AM377_RS21120 WP_108681987.1 4389047..4390069(-) (pilT) [Serratia marcescens strain AR_0130]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAACCGCTGGCGGCGGCGCAAACGCTGACGGCGCAAGGCGCGCAGGCCCTCTGCGATG
GATTGCTGAATGCGCAACAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGGGCGGGGAG
CGGTTGCGCGCCAACGTTTTTCAACAAAGCGCGGGGATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGCCGACGGATTGATCCTGATCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCATATTTTGACGCTGGAA
GATCCGATCGAGTTCCTGCATCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCACCTGGTGCTGACGACGCTGCATACCCGCAGCGCGCCGCAGGCGGTAGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCGGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCCGGCGGCGGACGAGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGTGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGTGTGTTGGGGGACTGCGCGGGGGAGGGAACGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

53.939

97.059

0.524
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.939

97.059

0.524
  pilT Acinetobacter baylyi ADP1

50.453

97.353

0.491
  pilT Acinetobacter nosocomialis M2

50

97.059

0.485
  pilT Acinetobacter baumannii D1279779

50

97.059

0.485
  pilT Acinetobacter baumannii strain A118

50

97.059

0.485
  pilT Neisseria meningitidis 8013

49.547

97.353

0.482
  pilT Neisseria gonorrhoeae MS11

49.245

97.353

0.479
  pilT Pseudomonas stutzeri DSM 10701

48.071

99.118

0.476
  pilT Pseudomonas aeruginosa PAK

48.788

97.059

0.474
  pilT Legionella pneumophila strain ERS1305867

48.64

97.353

0.474
  pilT Legionella pneumophila strain Lp02

48.64

97.353

0.474
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.38

100

0.453
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Vibrio cholerae strain A1552

38.957

95.882

0.374
  pilU Pseudomonas stutzeri DSM 10701

38.272

95.294

0.365

Multiple sequence alignment