Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CLC_RS11475 Genome accession   NC_009698
Coordinates   2521969..2523021 (-) Length   350 a.a.
NCBI ID   WP_011986859.1    Uniprot ID   -
Organism   Clostridium botulinum A str. Hall     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2516969..2528021
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLC_RS11460 (CLC_2393) ftsZ 2517494..2518603 (-) 1110 WP_003386373.1 cell division protein FtsZ -
  CLC_RS11465 (CLC_2394) ftsA 2518626..2519879 (-) 1254 WP_011986858.1 cell division protein FtsA -
  CLC_RS11470 (CLC_2395) - 2520036..2521310 (-) 1275 WP_003386376.1 glycosyltransferase family 2 protein -
  CLC_RS17560 (CLC_2396) - 2521748..2521891 (+) 144 WP_003386378.1 hypothetical protein -
  CLC_RS11475 (CLC_2397) pilT 2521969..2523021 (-) 1053 WP_011986859.1 type IV pilus twitching motility protein PilT Machinery gene
  CLC_RS11480 (CLC_2398) aroE 2523023..2523799 (-) 777 WP_011986860.1 shikimate dehydrogenase -
  CLC_RS11485 (CLC_2399) - 2523938..2524225 (-) 288 Protein_2280 sigma factor-like helix-turn-helix DNA-binding protein -
  CLC_RS11490 (CLC_2401) - 2524624..2524881 (+) 258 WP_242649199.1 helix-turn-helix domain-containing protein -
  CLC_RS11495 (CLC_2402) - 2525081..2525326 (+) 246 WP_011986863.1 helix-turn-helix domain-containing protein -
  CLC_RS11500 (CLC_2403) - 2525316..2525540 (+) 225 WP_011986864.1 hypothetical protein -
  CLC_RS11505 (CLC_2404) - 2525578..2526087 (-) 510 WP_003399443.1 lipoprotein -
  CLC_RS17565 (CLC_2405) - 2526155..2526304 (-) 150 WP_003399444.1 hypothetical protein -
  CLC_RS11510 (CLC_2406) mobV 2526282..2527190 (-) 909 WP_011986865.1 MobV family relaxase -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39041.11 Da        Isoelectric Point: 8.0876

>NTDB_id=28718 CLC_RS11475 WP_011986859.1 2521969..2523021(-) (pilT) [Clostridium botulinum A str. Hall]
MKQLKELLEKTINFGASDLHLSVGVEPTIRITGQLSKIFKEKLTPEDTNNYAKEILGEEFEDLLKKGEIDTSYSISGLGR
FRVSVFKQRGSIAISLRVVSLKIPNLNELKLPDIVKELTNKKRGLILVTGPTGSGKSTTLAAMINEINNTRSNHIITLED
PIEFLHKHNKCIINQREIGRDTFSYKSALKAALREDPDVILIGELRDLEDISIALTAAETGHLVFSTLHTIGGAKTIDRI
VNVFPPHQQEQIRIQVASVLEGIISQQLVHKIDGGRIAALETMVTTNAIRNMIREGKTYQIESSIQTGSKYGMKTMDMSL
AELYKREIISYDTALNYSVDKDLITKIINL

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=28718 CLC_RS11475 WP_011986859.1 2521969..2523021(-) (pilT) [Clostridium botulinum A str. Hall]
TTGAAACAATTAAAAGAATTATTGGAAAAAACTATTAACTTTGGTGCTTCAGATTTACATTTATCGGTAGGGGTAGAACC
AACTATAAGAATAACTGGACAATTAAGTAAAATATTTAAAGAAAAGCTTACACCAGAAGATACTAATAATTATGCCAAAG
AAATATTAGGTGAAGAATTTGAAGATTTATTGAAAAAAGGAGAAATAGATACATCCTATTCTATATCGGGCCTAGGTAGA
TTCAGAGTAAGCGTGTTTAAACAAAGGGGAAGTATAGCTATTTCTTTAAGGGTTGTAAGTTTGAAGATCCCTAACTTAAA
TGAACTAAAATTGCCTGATATAGTAAAGGAATTAACCAATAAAAAAAGAGGCCTTATTTTAGTAACGGGTCCTACAGGTT
CAGGCAAAAGCACTACTTTAGCAGCTATGATTAATGAAATTAATAATACTAGGTCTAATCATATAATAACTTTAGAAGAT
CCAATAGAATTTTTACATAAACATAACAAATGTATAATAAATCAAAGAGAGATAGGTAGGGATACTTTTAGTTATAAAAG
TGCTTTAAAAGCAGCATTAAGAGAAGATCCGGATGTAATATTAATAGGAGAGCTTAGAGATTTAGAAGATATTTCTATAG
CTTTAACAGCTGCAGAAACAGGACATTTAGTATTTTCTACTTTGCATACTATAGGAGGGGCTAAAACTATAGATAGAATT
GTTAATGTATTTCCGCCACATCAACAGGAGCAGATAAGAATACAAGTAGCAAGTGTGTTAGAAGGAATAATAAGTCAACA
ATTAGTACATAAAATTGATGGAGGAAGAATAGCAGCCTTAGAGACTATGGTAACTACAAATGCCATAAGAAATATGATAA
GGGAAGGAAAAACTTATCAAATAGAATCTTCCATACAAACTGGATCTAAATATGGAATGAAAACTATGGATATGTCCTTA
GCAGAGCTATATAAAAGAGAAATTATAAGCTACGATACTGCATTAAATTATTCAGTAGATAAGGATTTAATTACAAAAAT
AATTAATTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

50.751

95.143

0.483
  pilT Legionella pneumophila strain Lp02

48.696

98.571

0.48
  pilT Legionella pneumophila strain ERS1305867

48.696

98.571

0.48
  pilT Pseudomonas aeruginosa PAK

49.405

96

0.474
  pilT Acinetobacter baumannii D1279779

49.55

95.143

0.471
  pilT Acinetobacter nosocomialis M2

49.55

95.143

0.471
  pilT Acinetobacter baumannii strain A118

49.55

95.143

0.471
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

94.286

0.471
  pilT Vibrio cholerae strain A1552

50

94.286

0.471
  pilT Pseudomonas stutzeri DSM 10701

48.81

96

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.264

99.429

0.46
  pilT Neisseria gonorrhoeae MS11

47.463

95.714

0.454
  pilT Neisseria meningitidis 8013

47.463

95.714

0.454
  pilU Acinetobacter baylyi ADP1

40.828

96.571

0.394
  pilU Pseudomonas stutzeri DSM 10701

40.896

95.714

0.391
  pilU Vibrio cholerae strain A1552

38.643

96.857

0.374

Multiple sequence alignment