Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AOY83_RS04255 Genome accession   NZ_CP028745
Coordinates   879571..880551 (+) Length   326 a.a.
NCBI ID   WP_240767680.1    Uniprot ID   -
Organism   Escherichia coli strain A30     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 874571..885551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOY83_RS04225 (AOY83_04505) yggM 874998..876005 (+) 1008 WP_024228198.1 DUF1202 family protein -
  AOY83_RS04230 (AOY83_04510) hemW 876248..877384 (-) 1137 WP_187207595.1 radical SAM family heme chaperone HemW -
  AOY83_RS04235 (AOY83_04515) rdgB 877377..877970 (-) 594 WP_001174747.1 XTP/dITP diphosphatase -
  AOY83_RS04240 (AOY83_04520) yggU 877978..878268 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  AOY83_RS04245 (AOY83_04525) yggT 878265..878831 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  AOY83_RS04250 (AOY83_04530) yggS 878849..879553 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  AOY83_RS04255 (AOY83_04535) pilT 879571..880551 (+) 981 WP_240767680.1 type IV pilus twitching motility protein PilT Machinery gene
  AOY83_RS04260 (AOY83_04540) ruvX 880742..881158 (-) 417 WP_000017109.1 Holliday junction resolvase RuvX -
  AOY83_RS04265 (AOY83_04545) yqgE 881158..881721 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  AOY83_RS04270 (AOY83_04550) gshB 881830..882780 (-) 951 WP_000593273.1 glutathione synthase -
  AOY83_RS04275 (AOY83_04555) rsmE 882793..883524 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AOY83_RS04280 (AOY83_04560) endA 883604..884311 (-) 708 WP_098031165.1 deoxyribonuclease I -
  AOY83_RS04285 (AOY83_04565) yggI 884406..884903 (-) 498 WP_001300769.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35989.17 Da        Isoelectric Point: 5.5860

>NTDB_id=287114 AOY83_RS04255 WP_240767680.1 879571..880551(+) (pilT) [Escherichia coli strain A30]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFSVSLTENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGTPPILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=287114 AOY83_RS04255 WP_240767680.1 879571..880551(+) (pilT) [Escherichia coli strain A30]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTACGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTTCCGTGTCGCTGACGGAAAACCAGCGATTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CACACCACCGATATTGCCGGAGTTACTCAAGAGCGAGAATGGCTTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGTTATCTCAATCAACATGCTGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATCGGTTTGCATTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTTGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Neisseria meningitidis 8013

49.085

100

0.494
  pilT Neisseria gonorrhoeae MS11

48.78

100

0.491
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

47.095

100

0.472
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Vibrio cholerae strain A1552

40.063

97.239

0.39
  pilU Pseudomonas stutzeri DSM 10701

36.97

100

0.374
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371

Multiple sequence alignment