Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AOY85_RS18540 Genome accession   NZ_CP028743
Coordinates   3852574..3853554 (-) Length   326 a.a.
NCBI ID   WP_072017720.1    Uniprot ID   -
Organism   Escherichia coli strain A32     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3847574..3858554
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOY85_RS18510 (AOY85_19600) yggI 3848239..3848736 (+) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -
  AOY85_RS18515 (AOY85_19605) endA 3848831..3849538 (+) 708 WP_001305312.1 deoxyribonuclease I -
  AOY85_RS18520 (AOY85_19610) rsmE 3849618..3850349 (+) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AOY85_RS18525 (AOY85_19615) gshB 3850362..3851312 (+) 951 WP_000593259.1 glutathione synthase -
  AOY85_RS18530 (AOY85_19620) yqgE 3851421..3851984 (+) 564 WP_001301052.1 YqgE/AlgH family protein -
  AOY85_RS18535 (AOY85_19625) ruvX 3851984..3852400 (+) 417 WP_033549752.1 Holliday junction resolvase RuvX -
  AOY85_RS18540 (AOY85_19630) pilT 3852574..3853554 (-) 981 WP_072017720.1 type IV pilus twitching motility protein PilT Machinery gene
  AOY85_RS18545 (AOY85_19635) yggS 3853572..3854276 (+) 705 WP_000997807.1 pyridoxal phosphate homeostasis protein -
  AOY85_RS18550 (AOY85_19640) yggT 3854294..3854860 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  AOY85_RS18555 (AOY85_19645) yggU 3854857..3855147 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  AOY85_RS18560 (AOY85_19650) rdgB 3855155..3855748 (+) 594 WP_001174738.1 XTP/dITP diphosphatase -
  AOY85_RS18565 (AOY85_19655) hemW 3855741..3856877 (+) 1137 WP_033549754.1 radical SAM family heme chaperone HemW -
  AOY85_RS18570 (AOY85_19660) yggM 3856942..3857949 (-) 1008 WP_000745189.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36071.32 Da        Isoelectric Point: 5.9975

>NTDB_id=287081 AOY85_RS18540 WP_072017720.1 3852574..3853554(-) (pilT) [Escherichia coli strain A32]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAVPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VKEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=287081 AOY85_RS18540 WP_072017720.1 3852574..3853554(-) (pilT) [Escherichia coli strain A32]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGTGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACTTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCTGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGTTGTCACAAAAACTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Acinetobacter baylyi ADP1

37.267

98.773

0.368

Multiple sequence alignment