Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ANAE109_RS03430 Genome accession   NC_009675
Coordinates   751055..752152 (+) Length   365 a.a.
NCBI ID   WP_011984987.1    Uniprot ID   A7H835
Organism   Anaeromyxobacter sp. Fw109-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 746055..757152
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ANAE109_RS03405 (Anae109_0663) - 746292..747140 (+) 849 WP_011984982.1 purine-nucleoside phosphorylase -
  ANAE109_RS03410 (Anae109_0664) - 747187..747558 (+) 372 WP_011984983.1 PilZ domain-containing protein -
  ANAE109_RS03415 (Anae109_0665) - 747737..748153 (+) 417 WP_011984984.1 cytidine deaminase -
  ANAE109_RS03420 (Anae109_0666) ribF 748215..749183 (+) 969 WP_011984985.1 riboflavin biosynthesis protein RibF -
  ANAE109_RS03425 (Anae109_0667) pilB 749317..751023 (+) 1707 WP_011984986.1 type IV-A pilus assembly ATPase PilB -
  ANAE109_RS03430 (Anae109_0668) pilT 751055..752152 (+) 1098 WP_011984987.1 type IV pilus twitching motility protein PilT Machinery gene
  ANAE109_RS03435 (Anae109_0669) - 752214..753473 (+) 1260 WP_011984988.1 type II secretion system F family protein -
  ANAE109_RS03440 (Anae109_0670) - 753477..755129 (+) 1653 WP_234945233.1 nitrogen regulation protein NR(II) -
  ANAE109_RS03445 (Anae109_0671) - 755139..756512 (+) 1374 WP_011984990.1 sigma-54 dependent transcriptional regulator -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 40176.51 Da        Isoelectric Point: 8.9469

>NTDB_id=28671 ANAE109_RS03430 WP_011984987.1 751055..752152(+) (pilT) [Anaeromyxobacter sp. Fw109-5]
MANLHQLLKAMVEKGASDLHVTTGSPPQLRIDGALVPLKTPPLTPVETKQLCYSILTDSQKHKFEEESELDLSFGVKGLS
RFRANIFMQRGAVAGAFRTIPFKILTFQELGLPPIVTELAKKPRGLVLVTGPTGSGKSTTLASIIDKINTDRHEHIITIE
DPIEYLHPHKNCVVNQREVGADTQSFKKALKYILRQDPDVVLVGEMRDLETIEAALVISETGHLAFATLHTNSAVQTINR
ILDVFPPYQQPQVRAQLSFVLEGVLTQNLLPRAGGPGRVLIIEVMVPNPAIRNLIREDKIHQVYSQMQVGQAKFGMQTFN
QSLAAAFSRRLITLEDAMGRSPDPEELKNLLSSGAALRPAAPPSR

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=28671 ANAE109_RS03430 WP_011984987.1 751055..752152(+) (pilT) [Anaeromyxobacter sp. Fw109-5]
TTGGCGAACCTGCACCAGCTCCTCAAGGCGATGGTCGAGAAGGGGGCCTCCGACCTCCACGTGACGACCGGGTCGCCGCC
CCAGCTCCGCATCGACGGCGCCCTCGTGCCGCTCAAGACGCCGCCGCTCACGCCCGTCGAGACGAAGCAGCTCTGCTACT
CCATCCTGACGGACTCGCAGAAGCACAAGTTCGAGGAGGAGAGCGAGCTCGACCTCTCCTTCGGCGTGAAGGGGCTCTCG
CGCTTCCGGGCGAACATCTTCATGCAGCGCGGCGCCGTGGCCGGCGCGTTCCGCACCATCCCGTTCAAGATCCTGACCTT
TCAGGAGCTCGGGCTGCCGCCCATCGTGACCGAGCTCGCGAAGAAGCCGCGCGGCCTCGTGCTCGTGACCGGCCCCACCG
GCTCCGGCAAGTCGACCACGCTCGCGTCGATCATCGACAAGATCAACACGGACCGGCACGAGCACATCATCACGATCGAG
GATCCGATCGAGTACCTCCACCCGCACAAGAACTGCGTGGTGAACCAGCGCGAGGTCGGGGCCGACACCCAGTCGTTCAA
GAAGGCGCTCAAGTACATCCTCCGCCAGGATCCCGACGTGGTCCTCGTGGGCGAGATGCGCGACCTCGAGACCATCGAGG
CGGCGCTGGTCATCTCCGAGACCGGCCACCTGGCCTTCGCGACGCTGCACACGAACAGCGCGGTGCAGACCATCAACCGC
ATCCTGGACGTGTTCCCGCCCTACCAGCAGCCGCAGGTGCGCGCGCAGCTCTCGTTCGTGCTCGAGGGCGTGCTCACCCA
GAACCTGCTGCCTCGGGCCGGCGGTCCCGGGCGCGTGCTCATCATCGAGGTGATGGTCCCGAACCCCGCCATCCGGAACC
TGATCCGCGAGGACAAGATCCACCAGGTGTACTCGCAGATGCAGGTCGGCCAGGCCAAGTTCGGGATGCAGACCTTCAAC
CAGAGCCTCGCGGCGGCGTTCTCCCGGCGGCTCATCACGCTCGAGGACGCGATGGGCCGCTCCCCGGACCCCGAGGAGCT
GAAGAACCTGCTCTCGAGCGGCGCGGCCCTGCGCCCCGCCGCGCCGCCGTCCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7H835

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.316

100

0.526
  pilT Legionella pneumophila strain Lp02

53.474

90.685

0.485
  pilT Legionella pneumophila strain ERS1305867

53.474

90.685

0.485
  pilT Acinetobacter baylyi ADP1

50.145

94.521

0.474
  pilT Acinetobacter nosocomialis M2

50.145

94.521

0.474
  pilT Pseudomonas aeruginosa PAK

49.855

94.521

0.471
  pilT Acinetobacter baumannii strain A118

49.855

94.521

0.471
  pilT Acinetobacter baumannii D1279779

49.855

94.521

0.471
  pilT Pseudomonas stutzeri DSM 10701

48.986

94.521

0.463
  pilT Vibrio cholerae strain A1552

48.521

92.603

0.449
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.521

92.603

0.449
  pilT Neisseria gonorrhoeae MS11

46.921

93.425

0.438
  pilT Neisseria meningitidis 8013

46.921

93.425

0.438
  pilU Pseudomonas stutzeri DSM 10701

41.193

96.438

0.397
  pilU Vibrio cholerae strain A1552

41.143

95.89

0.395
  pilU Acinetobacter baylyi ADP1

40.058

95.068

0.381

Multiple sequence alignment