Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SUN_RS02500 Genome accession   NC_009663
Coordinates   493476..494534 (-) Length   352 a.a.
NCBI ID   WP_011980178.1    Uniprot ID   A6Q7I6
Organism   Sulfurovum sp. NBC37-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 488476..499534
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SUN_RS02480 (SUN_0481) mltB 489459..490478 (+) 1020 WP_011980174.1 lytic murein transglycosylase B -
  SUN_RS02485 (SUN_0482) lysS 490553..492196 (-) 1644 WP_011980175.1 lysine--tRNA ligase -
  SUN_RS02490 (SUN_0483) - 492342..492788 (-) 447 WP_011980176.1 Fur family transcriptional regulator -
  SUN_RS02495 (SUN_0484) - 492801..493421 (-) 621 WP_011980177.1 CvpA family protein -
  SUN_RS02500 (SUN_0485) pilT 493476..494534 (-) 1059 WP_011980178.1 type IV pilus twitching motility protein PilT Machinery gene
  SUN_RS02505 (SUN_0486) - 494554..494895 (-) 342 WP_011980179.1 arsenate reductase family protein -
  SUN_RS02510 (SUN_0487) gatC 494901..495191 (-) 291 WP_011980180.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  SUN_RS13735 - 495294..495638 (+) 345 WP_041672658.1 ferredoxin-thioredoxin reductase catalytic domain-containing protein -
  SUN_RS13740 (SUN_0488) - 495729..496073 (+) 345 WP_011980181.1 rhodanese-like domain-containing protein -
  SUN_RS02525 (SUN_0489) dapE 496085..497188 (+) 1104 WP_011980182.1 succinyl-diaminopimelate desuccinylase -
  SUN_RS02530 (SUN_0490) - 497193..497597 (+) 405 WP_011980183.1 thioredoxin family protein -
  SUN_RS02535 (SUN_0491) - 497661..498680 (+) 1020 WP_011980184.1 sensor histidine kinase -
  SUN_RS02540 (SUN_0492) - 498681..499526 (-) 846 WP_011980185.1 metallophosphoesterase family protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 38860.69 Da        Isoelectric Point: 6.4333

>NTDB_id=28577 SUN_RS02500 WP_011980178.1 493476..494534(-) (pilT) [Sulfurovum sp. NBC37-1]
MADFDIKKLLQSVVKHGASDLHLVSDAEPQIRLDGALKPVNLPKLTGEDIEEMCYSMITEKQKQHFEEYDELDFALYLPG
IGRFRANYYRTLGNVAAAFRVIPIEIPSLDDLGAPSIFKKLIKREKGLILVTGPTGSGKSTTLAALLNEINLYEQKHIIT
VEDPVEFIHTNKKCVFSHRGVGEDTKSFSAALKFAMRQDPDIILIGEMRDKETIEAALTAAETGHLVFGTLHTSSAPGTI
NRIIDVFTGDEQPQIRAMISTSLVAVIAQSLLPKLGGGRVASHEILVTNHAISNLIREDKVHQIYSQMQLGQEGTGMQTQ
SQALLKFIRDGKISRETAIQFANKPEELTKAL

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=28577 SUN_RS02500 WP_011980178.1 493476..494534(-) (pilT) [Sulfurovum sp. NBC37-1]
ATGGCCGATTTCGATATCAAAAAACTCTTACAAAGTGTTGTAAAACACGGTGCTTCAGACCTCCACCTGGTCTCAGATGC
GGAACCGCAGATAAGGCTTGACGGCGCACTCAAACCTGTTAACCTTCCAAAGCTGACCGGCGAAGATATTGAAGAGATGT
GTTATTCAATGATCACAGAAAAACAAAAACAGCATTTTGAAGAGTATGATGAACTTGATTTCGCCCTTTATCTACCGGGG
ATCGGCCGTTTCAGAGCGAACTACTATCGTACACTGGGCAATGTCGCAGCTGCTTTCAGGGTTATTCCCATTGAGATCCC
TTCTCTCGACGATCTTGGAGCACCATCGATCTTTAAAAAGCTGATCAAGCGGGAAAAAGGACTCATCCTCGTGACCGGGC
CTACAGGTTCAGGTAAGTCGACCACATTGGCTGCTCTCCTGAATGAGATCAACCTTTATGAGCAGAAACACATCATAACA
GTGGAAGATCCTGTGGAATTCATTCACACAAACAAAAAGTGTGTCTTCTCCCACAGAGGGGTTGGAGAAGATACCAAAAG
TTTTTCCGCAGCCCTCAAGTTTGCTATGCGTCAGGATCCCGATATCATCCTGATCGGGGAAATGAGGGACAAAGAGACGA
TTGAAGCGGCACTGACTGCGGCAGAGACAGGACACCTTGTATTCGGTACCCTACACACTTCTTCTGCACCCGGGACCATC
AACCGTATCATCGATGTTTTCACCGGTGATGAACAGCCACAAATCAGAGCTATGATCTCCACCTCGCTCGTTGCCGTCAT
TGCGCAGTCGCTTCTACCCAAACTCGGTGGAGGGCGTGTTGCTTCACATGAAATTCTTGTGACAAACCATGCGATCTCCA
ACCTGATCCGTGAAGACAAAGTACACCAGATCTATTCGCAGATGCAGCTCGGGCAGGAAGGTACAGGCATGCAGACCCAG
AGCCAGGCACTTCTCAAGTTTATCAGGGACGGAAAGATCAGTCGTGAGACAGCGATACAGTTCGCCAACAAACCCGAAGA
GCTTACTAAAGCCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A6Q7I6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

51.884

98.011

0.509
  pilT Acinetobacter baumannii D1279779

51.884

98.011

0.509
  pilT Acinetobacter baumannii strain A118

51.884

98.011

0.509
  pilT Legionella pneumophila strain Lp02

53.134

95.17

0.506
  pilT Legionella pneumophila strain ERS1305867

53.134

95.17

0.506
  pilT Pseudomonas stutzeri DSM 10701

51.304

98.011

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.333

93.75

0.5
  pilT Vibrio cholerae strain A1552

53.333

93.75

0.5
  pilT Pseudomonas aeruginosa PAK

50.725

98.011

0.497
  pilT Acinetobacter baylyi ADP1

50.435

98.011

0.494
  pilT Neisseria gonorrhoeae MS11

49.568

98.58

0.489
  pilT Neisseria meningitidis 8013

49.28

98.58

0.486
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.821

98.58

0.452
  pilU Acinetobacter baylyi ADP1

41.21

98.58

0.406
  pilU Pseudomonas stutzeri DSM 10701

39.017

98.295

0.384
  pilU Vibrio cholerae strain A1552

37.861

98.295

0.372

Multiple sequence alignment