Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NIS_RS06295 Genome accession   NC_009662
Coordinates   1190772..1191836 (+) Length   354 a.a.
NCBI ID   WP_012082547.1    Uniprot ID   A6Q475
Organism   Nitratiruptor sp. SB155-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1185772..1196836
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NIS_RS06260 (NIS_1168) - 1186567..1187247 (-) 681 WP_012082540.1 class I SAM-dependent DNA methyltransferase -
  NIS_RS06265 (NIS_1169) hisG 1187237..1187854 (-) 618 WP_012082541.1 ATP phosphoribosyltransferase -
  NIS_RS06270 (NIS_1170) - 1187848..1188456 (-) 609 WP_012082542.1 type III pantothenate kinase -
  NIS_RS06275 (NIS_1171) - 1188443..1188769 (-) 327 WP_012082543.1 hypothetical protein -
  NIS_RS06280 (NIS_1172) - 1188766..1189770 (-) 1005 WP_012082544.1 PQQ-binding-like beta-propeller repeat protein -
  NIS_RS06285 (NIS_1173) - 1189767..1190369 (-) 603 WP_012082545.1 tetratricopeptide repeat protein -
  NIS_RS06290 (NIS_1174) gatC 1190428..1190763 (+) 336 WP_012082546.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  NIS_RS06295 (NIS_1175) pilT 1190772..1191836 (+) 1065 WP_012082547.1 type IV pilus twitching motility protein PilT Machinery gene
  NIS_RS06300 (NIS_1176) - 1191849..1192469 (+) 621 WP_012082548.1 CvpA family protein -
  NIS_RS06305 (NIS_1177) lysS 1192475..1193980 (+) 1506 WP_041354270.1 lysine--tRNA ligase -
  NIS_RS06310 (NIS_1178) - 1193984..1195231 (+) 1248 WP_012082550.1 serine hydroxymethyltransferase -
  NIS_RS06315 (NIS_1179) - 1195312..1196043 (+) 732 WP_012082551.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39730.00 Da        Isoelectric Point: 7.1300

>NTDB_id=28568 NIS_RS06295 WP_012082547.1 1190772..1191836(+) (pilT) [Nitratiruptor sp. SB155-2]
MQIELTSLFKTVVAHKASDLHLVAGSEPQIRIDGRLVPLNLPKLEPKEVQELLYSLLTEKQKKIYEEEFELDFSFTVPNV
GRFRANYYRTLGAPAGAFRIIPMEIPTLDDLGAPQVFKKLVKREKGLILITGPTGSGKSTTLAAMLNEINETERKHIITI
EDPVEFLHKNKLSLFSHRELGLDTKSYANALKYALREDPDVILIGEMRDRETIHAALTAAETGHLVFGTLHTNSAPSTIN
RIIDVFPGDMQPQIRAQLAGSLVAAIAQSLLPKIGGGRIAVHEILINTPAIANLIRENKVHQIYSQMQLNQQETEMQTQT
KVLQKLLMQHVIDKETALQYANRPEELLNYIKHL

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=28568 NIS_RS06295 WP_012082547.1 1190772..1191836(+) (pilT) [Nitratiruptor sp. SB155-2]
ATGCAAATCGAACTCACCTCTTTATTTAAAACTGTCGTAGCTCACAAAGCTTCCGATTTGCACCTCGTTGCAGGAAGTGA
ACCGCAAATCCGTATTGATGGTAGACTTGTCCCACTCAATCTTCCAAAACTGGAACCCAAAGAGGTACAGGAACTGCTAT
ACAGCTTGCTGACAGAAAAACAGAAAAAAATATACGAAGAGGAGTTTGAGCTCGATTTCTCTTTTACCGTTCCAAATGTA
GGACGTTTTCGGGCAAACTACTATAGAACTCTGGGAGCTCCGGCAGGAGCCTTTCGTATCATTCCCATGGAGATCCCGAC
TCTCGATGATTTAGGGGCTCCACAGGTATTTAAAAAGCTCGTAAAGCGTGAGAAAGGGCTTATTCTCATTACCGGTCCTA
CTGGAAGCGGTAAATCAACAACGCTCGCTGCAATGCTCAATGAAATCAACGAAACAGAGCGAAAACATATCATCACCATC
GAAGATCCGGTAGAGTTCTTGCATAAAAACAAGCTCTCCCTATTCTCACATCGAGAACTGGGGCTCGACACGAAAAGCTA
TGCCAATGCATTGAAATATGCTCTTCGTGAGGACCCAGATGTTATCCTCATCGGTGAGATGCGAGATAGAGAAACGATTC
ATGCAGCCCTTACAGCCGCTGAAACGGGACACCTGGTTTTCGGTACATTGCACACAAATTCCGCACCAAGTACCATCAAT
AGGATCATCGATGTCTTTCCAGGTGACATGCAACCACAGATCAGAGCACAACTCGCTGGAAGTCTCGTAGCTGCAATTGC
GCAGTCTCTTTTGCCAAAAATCGGAGGAGGACGAATAGCCGTACACGAAATATTGATCAACACCCCTGCCATTGCGAACC
TCATTCGAGAAAACAAAGTACACCAGATCTATTCCCAAATGCAGCTCAACCAACAAGAGACGGAGATGCAGACACAAACA
AAAGTATTACAAAAGCTCCTTATGCAACATGTCATCGACAAAGAGACCGCTTTACAATATGCCAACCGTCCAGAAGAGTT
ACTCAACTATATCAAACACCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A6Q475

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

52.89

97.74

0.517
  pilT Acinetobacter baumannii D1279779

52.89

97.74

0.517
  pilT Acinetobacter baumannii strain A118

52.89

97.74

0.517
  pilT Neisseria gonorrhoeae MS11

52.023

97.74

0.508
  pilT Neisseria meningitidis 8013

51.734

97.74

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.453

94.068

0.503
  pilT Vibrio cholerae strain A1552

53.453

94.068

0.503
  pilT Pseudomonas aeruginosa PAK

51.304

97.458

0.5
  pilT Pseudomonas stutzeri DSM 10701

51.014

97.458

0.497
  pilT Acinetobacter baylyi ADP1

51.014

97.458

0.497
  pilT Legionella pneumophila strain ERS1305867

51.622

95.763

0.494
  pilT Legionella pneumophila strain Lp02

51.622

95.763

0.494
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.977

97.74

0.469
  pilU Pseudomonas stutzeri DSM 10701

40.456

99.153

0.401
  pilU Acinetobacter baylyi ADP1

39.773

99.435

0.395
  pilU Vibrio cholerae strain A1552

39.306

97.74

0.384

Multiple sequence alignment