Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CDG55_RS05175 Genome accession   NZ_CP028561
Coordinates   797162..798199 (-) Length   345 a.a.
NCBI ID   WP_087537169.1    Uniprot ID   A0A2R4TIE7
Organism   Acinetobacter sp. WCHA45     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 792162..803199
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG55_RS05145 (CDG55_05145) - 792818..793273 (+) 456 WP_004663432.1 bacteriohemerythrin -
  CDG55_RS05150 (CDG55_05150) - 793521..794603 (+) 1083 WP_087537166.1 hypothetical protein -
  CDG55_RS05155 (CDG55_05155) - 794603..794923 (+) 321 WP_087537167.1 RnfH family protein -
  CDG55_RS05160 (CDG55_05160) - 794977..795375 (-) 399 WP_087537168.1 outer membrane protein assembly factor BamE -
  CDG55_RS05165 (CDG55_05165) fur 795487..795924 (+) 438 WP_004663428.1 ferric iron uptake transcriptional regulator -
  CDG55_RS05170 (CDG55_05170) pilU 796019..797137 (-) 1119 WP_004663427.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CDG55_RS05175 (CDG55_05175) pilT 797162..798199 (-) 1038 WP_087537169.1 type IV pilus twitching motility protein PilT Machinery gene
  CDG55_RS05180 (CDG55_05180) - 798323..799000 (+) 678 WP_005158226.1 YggS family pyridoxal phosphate-dependent enzyme -
  CDG55_RS05185 (CDG55_05185) - 799113..802709 (-) 3597 WP_087537170.1 AAA family ATPase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38328.91 Da        Isoelectric Point: 6.3478

>NTDB_id=285511 CDG55_RS05175 WP_087537169.1 797162..798199(-) (pilT) [Acinetobacter sp. WCHA45]
MDITELLAFAVKNGASDLHLSSGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEDLETDFSFEVPNIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGSIFKEICDYPRGIVLVTGPTGSGKSTTLAAMIDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLVRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGVISPQVARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=285511 CDG55_RS05175 WP_087537169.1 797162..798199(-) (pilT) [Acinetobacter sp. WCHA45]
ATGGATATTACAGAATTACTAGCATTTGCAGTTAAAAACGGTGCTTCAGACTTACATCTTTCATCAGGTATGCCACCAAT
GATTCGTGTAGATGGTGAAGTCCGTCGTATTAATTTACCAGCACTAGAACATAAAGATGTACACCGCTTAGTGTATGACA
TCATGAATGATAAACAACGTCGTGATTATGAAGAAGATCTAGAAACTGACTTTTCATTTGAAGTGCCTAATATTGCACGT
TTCCGTGTGAACGCATTTAACCAAAACCGTGGCGCTGGCGCGGTATTCCGTACCATTCCATCTAAAGTTTTAACCATGGA
AGACCTTGGTTTAGGTTCAATTTTTAAAGAAATTTGTGATTATCCACGGGGTATCGTTTTGGTCACAGGACCTACGGGTT
CTGGTAAATCAACGACGCTCGCTGCGATGATCGACTATATCAACGAAAACCGCTATGACCACATTTTAACGGTTGAAGAC
CCAATCGAATTTGTACACCAATCTAAAAAATGCCTGATCAACCAGCGTGAAGTGCACCGTGATACACATGGCTTCAATGA
AGCACTGCGTTCAGCACTGCGTGAAGACCCTGACATTATCCTTGTGGGTGAGATGCGTGATTTGGAAACCATTCGTCTGG
CATTGACTGCTGCGGAAACGGGTCACTTGGTTTTTGGTACACTGCATACGACCTCTGCAGCGAAAACAATTGACCGTGTC
ATCGACGTATTCCCTGCCGAAGAAAAAGATATGGTTCGTGCCATGCTTTCTGAATCACTACAAGCCGTTATTTCACAAAC
ATTATTGAAGAAAAATGGTGGTGGTCGTGTTGCAGCACATGAAATCATGATTGGAATTCCAGCTATTCGTAACCTTGTAC
GTGAAAATAAAGTAGCTCAGATGTATTCTGCCATCCAAACGGGTGCAAACTACGGTATGACTACACTTGACCAAAGTCTT
AAGACCCTAGTTTCAAAAGGCGTTATTAGCCCTCAAGTTGCCCGTACTGCAGCAAAACAACCTGAGTCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2R4TIE7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

96.522

100

0.965
  pilT Acinetobacter baumannii D1279779

96.232

100

0.962
  pilT Acinetobacter baumannii strain A118

96.232

100

0.962
  pilT Acinetobacter baylyi ADP1

86.628

99.71

0.864
  pilT Pseudomonas stutzeri DSM 10701

82.849

99.71

0.826
  pilT Pseudomonas aeruginosa PAK

82.558

99.71

0.823
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Neisseria meningitidis 8013

68.696

100

0.687
  pilT Neisseria gonorrhoeae MS11

68.116

100

0.681
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.181

96.232

0.675
  pilT Vibrio cholerae strain A1552

70.181

96.232

0.675
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.507
  pilU Vibrio cholerae strain A1552

41.298

98.261

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.785

95.942

0.391
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388

Multiple sequence alignment