Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C7Y67_RS02150 Genome accession   NZ_CP028342
Coordinates   417699..418805 (+) Length   368 a.a.
NCBI ID   WP_025818783.1    Uniprot ID   A0A2S1M6K3
Organism   Vibrio parahaemolyticus isolate R13     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 412699..423805
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7Y67_RS02115 (C7Y67_02115) - 412769..413371 (-) 603 WP_025523187.1 XTP/dITP diphosphatase -
  C7Y67_RS02120 (C7Y67_02120) - 413489..413920 (-) 432 WP_005461703.1 DUF4426 domain-containing protein -
  C7Y67_RS02125 (C7Y67_02125) yggU 414048..414338 (-) 291 WP_108744735.1 DUF167 family protein YggU -
  C7Y67_RS02130 (C7Y67_02130) - 414338..414895 (-) 558 WP_005482440.1 YggT family protein -
  C7Y67_RS02135 (C7Y67_02135) proC 414948..415766 (-) 819 WP_108744736.1 pyrroline-5-carboxylate reductase -
  C7Y67_RS02140 (C7Y67_02140) - 415886..416599 (-) 714 WP_005461710.1 YggS family pyridoxal phosphate-dependent enzyme -
  C7Y67_RS02145 (C7Y67_02145) pilT 416628..417668 (+) 1041 WP_005461712.1 type IV pilus twitching motility protein PilT Machinery gene
  C7Y67_RS02150 (C7Y67_02150) pilU 417699..418805 (+) 1107 WP_025818783.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C7Y67_RS02155 (C7Y67_02155) ruvX 418867..419292 (-) 426 WP_005461715.1 Holliday junction resolvase RuvX -
  C7Y67_RS02160 (C7Y67_02160) - 419341..419904 (-) 564 WP_005461717.1 YqgE/AlgH family protein -
  C7Y67_RS02165 (C7Y67_02165) gshB 420018..420968 (-) 951 WP_005425823.1 glutathione synthase -
  C7Y67_RS02170 (C7Y67_02170) rsmE 420983..421714 (-) 732 WP_005461719.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  C7Y67_RS02175 (C7Y67_02175) endA 421839..422534 (-) 696 WP_005493878.1 deoxyribonuclease I -
  C7Y67_RS02180 (C7Y67_02180) - 422654..423151 (-) 498 WP_108744737.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41429.68 Da        Isoelectric Point: 7.0235

>NTDB_id=284248 C7Y67_RS02150 WP_025818783.1 417699..418805(+) (pilU) [Vibrio parahaemolyticus isolate R13]
MDLNKFLEGMLTLKASDLYITVGAPILFRVDGELRPQGEKLTEHDVAKLLDSAMEPDRRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETIIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVIGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEPFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=284248 C7Y67_RS02150 WP_025818783.1 417699..418805(+) (pilU) [Vibrio parahaemolyticus isolate R13]
ATGGATCTGAATAAATTTCTTGAAGGTATGTTGACGCTAAAAGCGTCGGATCTTTACATCACGGTTGGTGCGCCAATTCT
ATTTCGTGTGGATGGCGAATTGCGTCCGCAAGGGGAGAAACTCACCGAACATGATGTTGCAAAGTTGCTTGATAGCGCAA
TGGAGCCGGATCGGCGTCAGGAGTTTCGCAAAAGCCGCGAATCAAACTTTGCTATCGTCAGAGATTGTGGTCGCTTCCGT
GTTAGTGCTTTTTTTCAGCGAGAACTGCCGGGAGCAGTCATTCGCCGTATCGAAACCATTATTCCTACTTTTGAGCAGTT
AAAATTGCCTTTAGTGCTGCAAGACTTAGCGATTGCCAAGCGTGGTTTGGTGCTGGTGGTCGGCGCGACTGGCTCGGGTA
AATCGACCACGATGGCGGCGATGACGGGCTATCGAAACAGCAACAAAACCGGTCACATCTTGACGGTCGAAGATCCGATC
GAATTTGTGCATGAGCATAAGCGTTGTATCGTGACTCAGCGTGAGGTGGGTTTGGATACCGAAAGCTATGAAGTCGCACT
GAAAAACTCGTTGCGCCAAGCGCCAGATATGATTTTGATTGGCGAGATCCGTAGCCGAGAAACGATGGAATACGCGATGA
CCTTCGCCGAAACGGGTCACTTGTGTATGGCAACGTTGCACGCCAACAACGCCAACCAAGCGTTAGAGCGTATTCTCCAT
TTGGTGCCTAAAGATCAAAAAGAGCAATTCTTGTTTGATTTGTCGATGAACCTAAAAGGCGTGATTGGTCAGCAGCTGAT
CCGTGATAAGAATGGTCAAGGACGTCATGGTGTGTTTGAGATATTGCTAAACAGTCCACGAGTGTCGGATTTGATCCGTC
GTGGTGATTTACACGAATTGAAATCAACTATGGCACGATCCAATGAGTTTGGCATGCTGACGTTTGACCAATCGCTCTAC
AAGCTGGTGATGCAGGGTAAGATCAGCGAAGAGGATGCACTGCACAGTGCGGATTCAGCCAACGATCTTCGTTTAATGCT
GAAAACACAGCGTGGTGAGCCGTTCTCTACAGGCAGTCTTGCCAACGTCAAAATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S1M6K3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.88

100

0.829
  pilU Pseudomonas stutzeri DSM 10701

58

95.109

0.552
  pilU Acinetobacter baylyi ADP1

55.271

95.38

0.527
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.953

92.12

0.405
  pilT Legionella pneumophila strain ERS1305867

42.773

92.12

0.394
  pilT Legionella pneumophila strain Lp02

42.773

92.12

0.394
  pilT Acinetobacter nosocomialis M2

42.478

92.12

0.391
  pilT Acinetobacter baumannii D1279779

42.478

92.12

0.391
  pilT Acinetobacter baumannii strain A118

42.478

92.12

0.391
  pilT Pseudomonas aeruginosa PAK

42.183

92.12

0.389
  pilT Pseudomonas stutzeri DSM 10701

41.003

92.12

0.378
  pilT Acinetobacter baylyi ADP1

41.994

89.946

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Neisseria gonorrhoeae MS11

40.361

90.217

0.364
  pilT Neisseria meningitidis 8013

40.361

90.217

0.364

Multiple sequence alignment