Detailed information NTG0028399

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: AMET_RS17110
• Genome accession: NC_009633
• Coordinates: 3557807..3558862 (-)
• Length: 351 a.a.
• NCBI ID: WP_012064572.1
• Uniprot ID: A6TTU1
• Organism: Alkaliphilus metalliredigens QYMF
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3552807..3563862

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AMET_RS17090 (Amet_3477)	-	3553409..3554029 (-)	621	WP_012064568.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
AMET_RS17095 (Amet_3478)	-	3554087..3555811 (-)	1725	WP_012064569.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
AMET_RS26965 (Amet_3479)	-	3556172..3556510 (-)	339	WP_012064570.1	competence type IV pilus major pilin ComGC	-
AMET_RS17105 (Amet_3480)	-	3556547..3557764 (-)	1218	WP_012064571.1	type II secretion system F family protein	-
AMET_RS17110 (Amet_3481)	pilT	3557807..3558862 (-)	1056	WP_012064572.1	type IV pilus twitching motility protein PilT	Machinery gene
AMET_RS17115 (Amet_3482)	-	3558895..3560580 (-)	1686	WP_012064573.1	GspE/PulE family protein	-
AMET_RS17120 (Amet_3483)	-	3561184..3563469 (-)	2286	WP_049765299.1	PAS domain S-box protein	-

Sequence

Protein

• Length: 351 a.a.
• Molecular weight: 39105.54 Da
• Isoelectric Point: 8.4156

>NTDB_id=28399 AMET_RS17110 WP_012064572.1 3557807..3558862(-) (pilT) [Alkaliphilus metalliredigens QYMF]
MDILELLTKTVEAKASDLHITVASPPIVRINGKLVKMGEVKLMPEDTKRIVEKMMSPEQIILFHEKGELDFSYSNPGLGR
FRVNAYKQRGSYSMALRVVSLIIPTMEELDLPPIITELSKKKRGLILVTGPTGSGKSTTLAAMINYMNENRSEHILTLED
PIEYLHKHNKSIVNQREIGNDSYSFSNALRAALRQDPDVVLVGEMRDLETIAIAITAAETGHLVLSTLHTIGAAKTIDRI
IDVFPPHQQQQIKVQLSSVMEGVISQQLLPKADESGRVAAFEVMVSNPAIRNLIREGKTHQMQTVIQTKSKLGMKTMDAS
LMELYHKGIITREALQKHAVDLDMVNRQLGI

Nucleotide

• Length: 1056 bp

>NTDB_id=28399 AMET_RS17110 WP_012064572.1 3557807..3558862(-) (pilT) [Alkaliphilus metalliredigens QYMF]
TTGGATATATTAGAGCTATTAACTAAAACAGTAGAAGCAAAAGCTTCAGATCTACATATTACAGTAGCTTCGCCACCTAT
TGTTAGAATCAATGGCAAATTAGTTAAAATGGGAGAAGTTAAACTGATGCCAGAAGATACTAAAAGAATCGTAGAGAAGA
TGATGAGCCCAGAGCAGATAATCCTCTTTCATGAAAAAGGTGAACTTGATTTTTCATATTCAAATCCAGGTTTAGGTAGG
TTTAGAGTCAATGCATATAAACAAAGAGGAAGCTATTCTATGGCACTTCGTGTTGTGTCTTTGATAATACCAACAATGGA
GGAACTGGATCTACCTCCGATAATAACGGAACTGTCGAAGAAAAAACGAGGACTGATATTAGTGACAGGTCCAACAGGTA
GTGGAAAGTCCACGACCTTGGCAGCGATGATTAACTATATGAATGAAAATAGAAGTGAACATATTTTAACCCTTGAAGAC
CCCATTGAGTATTTACATAAGCATAACAAGAGTATTGTCAATCAAAGAGAAATAGGAAATGACTCTTACAGCTTTTCTAA
TGCATTAAGAGCAGCACTAAGGCAAGATCCAGACGTTGTTTTAGTGGGAGAGATGAGGGATTTAGAGACCATTGCCATTG
CCATTACCGCTGCGGAAACTGGACATTTAGTATTATCCACTTTACATACAATCGGTGCTGCTAAAACAATAGACAGAATC
ATCGACGTATTTCCTCCCCATCAACAGCAGCAGATTAAAGTGCAACTGTCATCTGTAATGGAAGGGGTGATTTCCCAACA
GCTCCTACCAAAAGCAGATGAATCTGGAAGAGTTGCAGCTTTTGAAGTCATGGTTAGCAATCCTGCCATAAGAAACTTAA
TTCGAGAAGGAAAAACCCACCAGATGCAAACTGTGATTCAGACAAAATCAAAGCTAGGAATGAAGACAATGGATGCTTCA
TTAATGGAACTGTATCATAAAGGAATAATTACTAGAGAAGCCCTACAAAAGCATGCAGTAGACTTAGATATGGTCAACCG
TCAATTAGGTATTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (46-271)

3D structure

• AlphaFold DB: A6TTU1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	50.581	98.006	0.496
pilT	Vibrio cholerae strain A1552	50.581	98.006	0.496
pilT	Legionella pneumophila strain ERS1305867	50.733	97.151	0.493
pilT	Legionella pneumophila strain Lp02	50.733	97.151	0.493
pilT	Acinetobacter baumannii strain A118	51.36	94.302	0.484
pilT	Acinetobacter nosocomialis M2	51.36	94.302	0.484
pilT	Acinetobacter baumannii D1279779	51.36	94.302	0.484
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	49.853	97.151	0.484
pilT	Pseudomonas aeruginosa PAK	49.853	96.581	0.481
pilT	Acinetobacter baylyi ADP1	51.212	94.017	0.481
pilT	Pseudomonas stutzeri DSM 10701	48.968	96.581	0.473
pilT	Neisseria gonorrhoeae MS11	48.083	96.581	0.464
pilT	Neisseria meningitidis 8013	48.083	96.581	0.464
pilU	Pseudomonas stutzeri DSM 10701	44.543	96.581	0.43
pilU	Acinetobacter baylyi ADP1	41.642	97.151	0.405
pilU	Vibrio cholerae strain A1552	42.593	92.308	0.393