Detailed information NTG0028131

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: GURA_RS13640
• Genome accession: NC_009483
• Coordinates: 3113959..3115065 (-)
• Length: 368 a.a.
• NCBI ID: WP_011939531.1
• Uniprot ID: A5G4Y7
• Organism: Geotalea uraniireducens Rf4
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3108959..3120065

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GURA_RS25365	-	3109206..3109320 (-)	115	Protein_2748	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
GURA_RS13625 (Gura_2678)	-	3109593..3110966 (-)	1374	WP_011939528.1	sigma-54 dependent transcriptional regulator	-
GURA_RS13630 (Gura_2679)	-	3111044..3112678 (-)	1635	WP_011939529.1	nitrogen regulation protein NR(II)	-
GURA_RS13635 (Gura_2680)	-	3112682..3113899 (-)	1218	WP_011939530.1	type II secretion system F family protein	-
GURA_RS13640 (Gura_2681)	pilT	3113959..3115065 (-)	1107	WP_011939531.1	type IV pilus twitching motility protein PilT	Machinery gene
GURA_RS13645 (Gura_2682)	pilB	3115078..3116784 (-)	1707	WP_011939532.1	type IV-A pilus assembly ATPase PilB	-
GURA_RS13650 (Gura_2683)	aroE	3116854..3117723 (-)	870	WP_011939533.1	shikimate dehydrogenase	-
GURA_RS13655 (Gura_2684)	-	3117720..3119585 (-)	1866	WP_011939534.1	DUF2723 domain-containing protein	-

Sequence

Protein

• Length: 368 a.a.
• Molecular weight: 41059.40 Da
• Isoelectric Point: 7.5354

>NTDB_id=28131 GURA_RS13640 WP_011939531.1 3113959..3115065(-) (pilT) [Geotalea uraniireducens Rf4]
MANLHQLLKELVERGGSDLHITTNTPPQIRIDGKITPMDFPPLTPVETKQLCYSILTDAQKHKFEEESELDLSFGVKGLS
RFRGNVFVQRGAVAGVFRVIPYKILTFEELGLPPIVRELAAKPRGLVLVTGPTGSGKSTTLASIIDKINMDRHDHIVTIE
DPIEYLHPHKGCLVNQREVGADTKSFRNALKYVLRQDPDVVLIGELRDLETIEAALTLAETGHLCFATLHTNSCAQTINR
IIDVFPPYQQTQVRTQLSFVLEGVLSQTLVPRASGHGRALALEVMVPNPAIRNLIREDKVHQIYSQMQVGQDKFGMMTLN
QSLYGLFTKRIISLDDAMARSPEPDELKQMVSVGSVQSQVRRPLTDKR

Nucleotide

• Length: 1107 bp

>NTDB_id=28131 GURA_RS13640 WP_011939531.1 3113959..3115065(-) (pilT) [Geotalea uraniireducens Rf4]
ATGGCGAATTTGCATCAACTTTTAAAAGAGCTTGTTGAAAGAGGAGGATCGGACCTCCATATCACGACAAACACCCCGCC
ACAGATAAGAATTGACGGTAAAATTACTCCGATGGATTTTCCTCCTCTTACACCGGTCGAAACCAAGCAGCTGTGCTACA
GCATCCTGACTGATGCGCAAAAACATAAGTTCGAGGAGGAGAGCGAACTCGACCTGTCCTTTGGCGTCAAGGGGTTGTCG
CGCTTCAGGGGGAATGTATTTGTCCAGCGGGGAGCGGTTGCCGGAGTATTCAGGGTCATACCCTACAAGATTCTCACGTT
TGAAGAACTGGGACTGCCGCCGATAGTAAGGGAGCTTGCTGCCAAGCCAAGAGGTCTCGTTCTCGTCACCGGACCGACCG
GCAGCGGCAAGTCGACCACCCTGGCCTCCATTATTGATAAAATAAATATGGACCGCCACGATCACATCGTTACGATAGAA
GATCCGATAGAATATCTCCATCCACATAAGGGGTGCCTGGTCAACCAGCGCGAGGTCGGGGCGGACACCAAGAGCTTCAG
AAACGCCCTCAAATACGTTTTGCGTCAGGACCCTGACGTCGTACTAATCGGTGAGCTCAGGGATCTTGAGACAATTGAGG
CCGCCCTGACGCTGGCTGAAACCGGGCATCTTTGCTTCGCTACTCTCCATACCAATTCCTGCGCACAGACAATCAACCGC
ATCATAGATGTGTTCCCACCCTACCAGCAAACCCAGGTCAGGACACAGCTCTCCTTTGTCCTGGAAGGGGTTCTGTCTCA
AACCCTCGTTCCGAGAGCAAGCGGCCATGGCAGAGCGCTTGCCTTGGAGGTGATGGTTCCGAACCCCGCGATCCGCAACC
TGATCCGGGAGGATAAGGTTCATCAGATTTATTCCCAGATGCAGGTCGGGCAGGATAAATTCGGCATGATGACCTTGAAC
CAATCGCTGTACGGTCTTTTTACTAAACGTATCATTTCTCTCGATGATGCGATGGCCAGATCACCTGAGCCTGATGAGCT
GAAACAGATGGTATCTGTCGGCAGTGTGCAGAGCCAGGTGCGAAGGCCTCTCACGGATAAAAGATAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (9-261)

3D structure

• AlphaFold DB: A5G4Y7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Acinetobacter baylyi ADP1	53.936	93.207	0.503
pilT	Legionella pneumophila strain Lp02	54.985	89.946	0.495
pilT	Legionella pneumophila strain ERS1305867	54.985	89.946	0.495
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	50	96.196	0.481
pilT	Pseudomonas aeruginosa PAK	51.014	93.75	0.478
pilT	Acinetobacter nosocomialis M2	51.014	93.75	0.478
pilT	Acinetobacter baumannii D1279779	51.014	93.75	0.478
pilT	Acinetobacter baumannii strain A118	51.014	93.75	0.478
pilT	Pseudomonas stutzeri DSM 10701	50.725	93.75	0.476
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	48.225	91.848	0.443
pilT	Vibrio cholerae strain A1552	48.225	91.848	0.443
pilT	Neisseria meningitidis 8013	46.847	90.489	0.424
pilT	Neisseria gonorrhoeae MS11	46.246	90.489	0.418
pilU	Pseudomonas stutzeri DSM 10701	42.898	95.652	0.41
pilU	Vibrio cholerae strain A1552	41.954	94.565	0.397
pilU	Acinetobacter baylyi ADP1	39.617	99.457	0.394