Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C1N55_RS07555 Genome accession   NZ_CP028083
Coordinates   1534006..1535046 (+) Length   346 a.a.
NCBI ID   WP_137728250.1    Uniprot ID   A0A4P8S2W8
Organism   Lysinibacillus sp. SGAir0095     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1529006..1540046
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1N55_RS07535 (C1N55_07555) - 1529539..1529964 (-) 426 WP_137728246.1 type II secretion system protein -
  C1N55_RS07540 (C1N55_07560) - 1529951..1530451 (-) 501 WP_137728247.1 hypothetical protein -
  C1N55_RS07545 (C1N55_07565) - 1530933..1532312 (+) 1380 WP_137728248.1 VanW family protein -
  C1N55_RS07550 (C1N55_07570) - 1532336..1533994 (+) 1659 WP_370452614.1 GspE/PulE family protein -
  C1N55_RS07555 (C1N55_07575) pilT 1534006..1535046 (+) 1041 WP_137728250.1 type IV pilus twitching motility protein PilT Machinery gene
  C1N55_RS07560 (C1N55_07580) - 1535065..1536273 (+) 1209 WP_137728251.1 type II secretion system F family protein -
  C1N55_RS07565 (C1N55_07585) - 1536387..1536845 (+) 459 WP_205758523.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  C1N55_RS07570 (C1N55_07590) pilM 1536947..1537927 (+) 981 WP_137728252.1 type IV pilus biogenesis protein PilM -
  C1N55_RS07575 (C1N55_07595) - 1537924..1538511 (+) 588 WP_137728253.1 malate synthase -
  C1N55_RS07580 (C1N55_07600) pilO 1538511..1539242 (+) 732 WP_240758404.1 type 4a pilus biogenesis protein PilO -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38624.25 Da        Isoelectric Point: 6.5782

>NTDB_id=281144 C1N55_RS07555 WP_137728250.1 1534006..1535046(+) (pilT) [Lysinibacillus sp. SGAir0095]
MFSIRSLLERAYEEKASDLHITVGIPPVYRINGKLKQFGDYAVIPEEADALVREVLPEYKVEEYVKKGESDFNFSLGDLC
RFRVNAYHQRNVSAVAFRLIPSKIPTIESLGMPKVLYDLADKPQGLILVTGPTGSGKSTTLAAMLDYINETKSKHIITLE
DPIEYLHHHKQSVVNQREIGIDTQDFASGLRASLRQDPDVILVGEMRDLETISTAITAAETGHLVFATLHTSSAPTTIDR
IIDVFPPHQQGQIRIQLANVLQGIISQRLFPRKDMNGRIAATEILTALPSVTNLIRNEKVHQIPSVMQTSRAMGMHTLET
SIQSLVSSGKVSMEEARPYLNFDDYK

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=281144 C1N55_RS07555 WP_137728250.1 1534006..1535046(+) (pilT) [Lysinibacillus sp. SGAir0095]
ATGTTTAGTATACGTTCGTTATTAGAAAGAGCTTATGAAGAAAAAGCATCGGACTTGCACATTACGGTTGGAATTCCACC
CGTTTATCGTATAAATGGAAAGCTTAAGCAATTTGGAGACTATGCTGTTATTCCAGAGGAAGCTGACGCACTTGTAAGAG
AGGTGCTGCCGGAATATAAGGTGGAGGAGTATGTGAAAAAGGGGGAATCAGATTTTAATTTCTCCCTAGGTGATTTATGT
CGTTTCCGTGTAAACGCCTATCATCAGCGGAATGTGAGTGCGGTTGCCTTTCGTTTAATTCCATCGAAAATCCCGACGAT
TGAATCTCTTGGGATGCCTAAAGTCCTTTATGATTTAGCGGATAAGCCACAAGGACTGATTTTAGTAACCGGTCCAACTG
GTTCTGGTAAATCGACGACACTCGCTGCAATGCTGGATTATATAAATGAAACAAAATCAAAGCATATTATAACCCTTGAG
GATCCAATTGAGTATTTACATCATCATAAACAATCTGTTGTGAATCAACGTGAAATCGGCATTGATACTCAAGACTTTGC
GAGTGGATTGCGTGCATCTCTTCGTCAGGATCCGGACGTAATTTTAGTTGGGGAGATGCGAGACTTAGAAACAATATCTA
CGGCAATTACTGCAGCTGAAACAGGGCATTTAGTGTTTGCAACACTTCACACATCTAGTGCTCCAACTACAATCGACCGT
ATCATTGATGTGTTTCCACCGCATCAGCAAGGGCAAATTCGAATACAGCTTGCAAACGTGTTGCAAGGCATCATTTCGCA
AAGATTGTTTCCCCGCAAAGATATGAATGGCCGTATTGCTGCTACAGAAATCTTAACTGCATTACCTTCTGTAACAAATT
TGATTCGAAACGAAAAAGTACATCAAATTCCAAGTGTCATGCAAACAAGCAGAGCAATGGGAATGCATACTTTAGAAACT
TCAATTCAATCACTTGTTTCTTCGGGTAAGGTATCGATGGAAGAAGCACGCCCTTATCTGAATTTTGACGACTATAAGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(78-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P8S2W8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

50

96.532

0.483
  pilT Neisseria gonorrhoeae MS11

49.701

96.532

0.48
  pilT Acinetobacter nosocomialis M2

49.405

97.11

0.48
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.553

93.064

0.48
  pilT Acinetobacter baumannii D1279779

49.107

97.11

0.477
  pilT Acinetobacter baumannii strain A118

49.107

97.11

0.477
  pilT Acinetobacter baylyi ADP1

49.107

97.11

0.477
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.529

98.266

0.477
  pilT Vibrio cholerae strain A1552

48.529

98.266

0.477
  pilT Pseudomonas aeruginosa PAK

49.102

96.532

0.474
  pilT Pseudomonas stutzeri DSM 10701

48.503

96.532

0.468
  pilT Legionella pneumophila strain ERS1305867

46.847

96.243

0.451
  pilT Legionella pneumophila strain Lp02

46.847

96.243

0.451
  pilU Pseudomonas stutzeri DSM 10701

42.727

95.376

0.408
  pilU Vibrio cholerae strain A1552

41.925

93.064

0.39
  pilU Acinetobacter baylyi ADP1

38.066

95.665

0.364

Multiple sequence alignment