Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C7M79_RS05950 Genome accession   NZ_CP027775
Coordinates   1332887..1333939 (+) Length   350 a.a.
NCBI ID   WP_033060507.1    Uniprot ID   A0A7U4LNK1
Organism   Clostridium botulinum strain MFBjulcb1     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1327887..1338939
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7M79_RS05930 (C7M79_06060) udk 1328793..1329413 (+) 621 WP_003495316.1 uridine kinase -
  C7M79_RS05935 (C7M79_06065) - 1329499..1331169 (+) 1671 WP_003495318.1 penicillin-binding protein 2 -
  C7M79_RS05940 (C7M79_06070) sigK 1331266..1331970 (+) 705 WP_003484649.1 RNA polymerase sporulation sigma factor SigK -
  C7M79_RS05945 (C7M79_06075) aroE 1332109..1332885 (+) 777 WP_033060510.1 shikimate dehydrogenase -
  C7M79_RS05950 (C7M79_06080) pilT 1332887..1333939 (+) 1053 WP_033060507.1 type IV pilus twitching motility protein PilT Machinery gene
  C7M79_RS05955 (C7M79_06085) - 1334051..1334185 (-) 135 WP_033060504.1 hypothetical protein -
  C7M79_RS05960 (C7M79_06090) - 1334624..1335898 (+) 1275 WP_003494428.1 glycosyltransferase family 2 protein -
  C7M79_RS05965 (C7M79_06095) ftsA 1336054..1337307 (+) 1254 WP_033060501.1 cell division protein FtsA -
  C7M79_RS05970 (C7M79_06100) ftsZ 1337330..1338439 (+) 1110 WP_003494426.1 cell division protein FtsZ -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39053.12 Da        Isoelectric Point: 8.6835

>NTDB_id=280502 C7M79_RS05950 WP_033060507.1 1332887..1333939(+) (pilT) [Clostridium botulinum strain MFBjulcb1]
MKQLKELLEKTINFGASDLHLSVGVEPTIRINGQLSKIFKEKLTPENTNNYAKEILGEEFEDLLKKGEIDTSYSISGLGR
FRVSVFKQRGSIAISLRVVSLKIPNLNELKLPDIVKELTNKKRGLILVTGPTGSGKSTTLAAMINEINNTRSNHIITLED
PIEFLHKHNKCIINQREIGRDTFSYKSALKAALREDPDVILIGELRDLEDISIALTAAETGHLVFSTLHTIGGAKTIDRI
VNVFPPHQQEQIRIQVASVLEGIISQQLVHKIDGGRIAALETMITTNAIRNMIREGKTYQIESSIQTGSKYGMKTMDMSL
AELYKREVISYDTALNYSVDKDLITKIINL

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=280502 C7M79_RS05950 WP_033060507.1 1332887..1333939(+) (pilT) [Clostridium botulinum strain MFBjulcb1]
TTGAAACAATTAAAAGAATTATTGGAAAAAACTATTAACTTTGGCGCTTCAGATTTACATTTATCCGTAGGGGTAGAACC
AACTATAAGAATAAATGGTCAATTAAGCAAAATATTCAAAGAAAAGCTTACACCAGAAAATACTAATAATTATGCTAAAG
AAATACTAGGGGAAGAATTTGAGGATTTATTAAAAAAAGGAGAAATAGATACATCCTATTCTATATCAGGCTTAGGTAGA
TTTAGAGTAAGTGTGTTTAAACAAAGGGGAAGTATAGCTATTTCTTTAAGGGTTGTAAGTTTAAAGATTCCTAACTTAAA
TGAATTAAAATTACCTGATATAGTAAAGGAATTAACAAATAAAAAGAGAGGCCTTATTTTAGTAACAGGTCCTACAGGTT
CAGGTAAAAGCACTACTTTAGCAGCTATGATTAATGAAATTAATAATACTAGATCTAATCATATAATAACTTTGGAAGAT
CCAATAGAATTTTTACATAAACATAACAAATGTATAATAAATCAAAGGGAGATAGGTAGAGATACTTTTAGTTATAAAAG
CGCTTTAAAAGCAGCTTTAAGAGAAGATCCAGATGTAATATTAATAGGAGAACTTAGGGATTTAGAAGATATTTCTATAG
CTTTAACAGCTGCAGAAACAGGACATCTAGTGTTTTCTACTTTGCATACTATAGGAGGAGCTAAGACTATAGACAGAATT
GTTAATGTATTTCCACCACATCAACAAGAACAGATAAGAATACAAGTAGCAAGTGTGTTAGAGGGAATAATAAGTCAACA
ATTAGTACATAAAATTGATGGAGGAAGAATAGCAGCTTTAGAGACCATGATAACTACAAATGCCATAAGAAATATGATAA
GAGAAGGAAAAACTTATCAAATAGAATCTTCCATACAAACTGGATCCAAATATGGAATGAAAACTATGGATATGTCTTTA
GCAGAGTTATATAAAAGAGAAGTTATAAGTTATGATACTGCATTAAATTATTCAGTAGACAAAGATTTAATTACAAAGAT
AATTAATTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(92-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U4LNK1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

50.751

95.143

0.483
  pilT Pseudomonas aeruginosa PAK

49.702

96

0.477
  pilT Acinetobacter baumannii strain A118

49.85

95.143

0.474
  pilT Acinetobacter baumannii D1279779

49.85

95.143

0.474
  pilT Acinetobacter nosocomialis M2

49.85

95.143

0.474
  pilT Legionella pneumophila strain Lp02

48.116

98.571

0.474
  pilT Legionella pneumophila strain ERS1305867

48.116

98.571

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

94.286

0.471
  pilT Vibrio cholerae strain A1552

50

94.286

0.471
  pilT Pseudomonas stutzeri DSM 10701

49.107

96

0.471
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.977

99.429

0.457
  pilT Neisseria meningitidis 8013

47.761

95.714

0.457
  pilT Neisseria gonorrhoeae MS11

47.761

95.714

0.457
  pilU Acinetobacter baylyi ADP1

41.42

96.571

0.4
  pilU Pseudomonas stutzeri DSM 10701

41.493

95.714

0.397
  pilU Vibrio cholerae strain A1552

38.348

96.857

0.371

Multiple sequence alignment