Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C4J83_RS28430 Genome accession   NZ_CP027762
Coordinates   6111558..6112592 (-) Length   344 a.a.
NCBI ID   WP_119737449.1    Uniprot ID   -
Organism   Pseudomonas sp. LBUM920     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6106558..6117592
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4J83_RS28400 (C4J83_5631) ruvX 6106642..6107079 (+) 438 WP_003195067.1 Holliday junction resolvase RuvX -
  C4J83_RS28405 (C4J83_5632) pyrR 6107152..6107658 (+) 507 WP_106575674.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  C4J83_RS28410 (C4J83_5633) - 6107683..6108687 (+) 1005 WP_106575675.1 aspartate carbamoyltransferase catalytic subunit -
  C4J83_RS28415 (C4J83_5634) - 6108684..6109955 (+) 1272 WP_106575676.1 dihydroorotase -
  C4J83_RS28420 (C4J83_5635) - 6110101..6110529 (-) 429 WP_119737451.1 NINE protein -
  C4J83_RS28425 (C4J83_5636) - 6110779..6111417 (+) 639 WP_106575678.1 C40 family peptidase -
  C4J83_RS28430 (C4J83_5637) pilT 6111558..6112592 (-) 1035 WP_119737449.1 type IV pilus twitching motility protein PilT Machinery gene
  C4J83_RS28435 (C4J83_5638) - 6112650..6113336 (+) 687 WP_124418661.1 YggS family pyridoxal phosphate-dependent enzyme -
  C4J83_RS28440 (C4J83_5639) proC 6113370..6114188 (+) 819 WP_124418662.1 pyrroline-5-carboxylate reductase -
  C4J83_RS28445 (C4J83_5640) - 6114224..6114811 (+) 588 WP_106575682.1 YggT family protein -
  C4J83_RS28450 (C4J83_5641) - 6115034..6116173 (+) 1140 WP_106575683.1 homoserine O-acetyltransferase -
  C4J83_RS28455 (C4J83_5642) metW 6116181..6116801 (+) 621 WP_003213768.1 methionine biosynthesis protein MetW -
  C4J83_RS28460 (C4J83_5643) - 6116823..6117257 (+) 435 WP_106575685.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37313.18 Da        Isoelectric Point: 8.6189

>NTDB_id=280327 C4J83_RS28430 WP_119737449.1 6111558..6112592(-) (pilT) [Pseudomonas sp. LBUM920]
MDITELLTASVRRGASDLHLSAGLAPMLRVDGEVWPLEGPVLTPPQVADLLSPLLNSYQQKDFKTSLETDFAFELPSVAR
FRVNVFQQDRGMGAVFRTIPAQVRSLESLGLGAVFQRIAQLPRGLVLVTGPTGSGKSTTLAAMIDYLNGHRRQHILTLED
PIEFIHRPKMALINQRQVHRDTHSFSAALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKSVDRL
VDVFPAAEKAMVRSMLSESLQAVVSQVLVKKIGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KALVSEGLISREDARGQARVPGDI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=280327 C4J83_RS28430 WP_119737449.1 6111558..6112592(-) (pilT) [Pseudomonas sp. LBUM920]
ATGGATATCACTGAATTGCTGACGGCCAGCGTGCGCCGTGGCGCCTCCGACCTGCATTTGTCGGCCGGCCTGGCACCGAT
GCTGCGTGTCGATGGCGAGGTCTGGCCGCTGGAAGGCCCGGTGCTCACGCCCCCGCAAGTTGCGGACTTATTGAGCCCTT
TGCTCAACAGCTACCAACAAAAGGATTTCAAAACATCTCTTGAAACCGATTTTGCCTTTGAACTGCCGAGCGTGGCGCGG
TTCCGGGTGAACGTATTTCAGCAGGACCGCGGCATGGGGGCGGTGTTTCGCACCATTCCCGCCCAGGTGCGGAGCCTTGA
AAGCCTCGGGCTGGGGGCGGTGTTCCAGCGGATCGCGCAACTGCCGCGCGGCCTGGTGCTGGTCACCGGGCCGACCGGGT
CGGGCAAGTCCACCACGCTGGCGGCGATGATCGACTACTTAAACGGGCATCGACGCCAGCACATCCTCACCCTCGAAGAC
CCCATCGAATTTATCCACAGGCCGAAAATGGCCTTGATCAACCAGCGCCAGGTGCACCGCGATACCCACAGCTTTTCGGC
GGCGCTGCGTTCGGCTCTGCGTGAGGACCCGGATGTGATTCTGGTGGGCGAATTGCGCGACCTTGAGACCATCCGCCTGG
CGCTGACCGCCGCTGAGACCGGGCACCTGGTATTTGGCACCCTGCACACCACGTCGGCCGCAAAGAGCGTGGACCGGCTG
GTGGACGTGTTCCCGGCAGCGGAAAAGGCCATGGTGCGCTCGATGCTCTCGGAATCGTTGCAGGCGGTGGTGTCGCAAGT
GCTGGTCAAGAAAATCGGCGGCGGGCGGGTGGCGGCTCACGAGATCATGCTGGGGACACCGGCTATTCGTAACTTGATCC
GCGAAGACAAAGTGGCGCAGATGGTCTCGGCGATCCAGACCGGTGGGGCACTGGGGATGAAAACGTTGGACATGAGCTTG
AAGGCGTTGGTCAGCGAGGGGCTGATCAGCCGGGAAGATGCGCGGGGGCAGGCGAGGGTGCCTGGGGATATTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

73.607

99.128

0.73
  pilT Pseudomonas stutzeri DSM 10701

71.261

99.128

0.706
  pilT Acinetobacter nosocomialis M2

70.381

99.128

0.698
  pilT Acinetobacter baumannii D1279779

70.088

99.128

0.695
  pilT Acinetobacter baumannii strain A118

70.088

99.128

0.695
  pilT Acinetobacter baylyi ADP1

69.795

99.128

0.692
  pilT Legionella pneumophila strain Lp02

63.905

98.256

0.628
  pilT Legionella pneumophila strain ERS1305867

63.905

98.256

0.628
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

63.798

97.965

0.625
  pilT Vibrio cholerae strain A1552

63.798

97.965

0.625
  pilT Neisseria meningitidis 8013

62.059

98.837

0.613
  pilT Neisseria gonorrhoeae MS11

61.765

98.837

0.61
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.751

96.802

0.491
  pilU Vibrio cholerae strain A1552

40

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

39.42

100

0.395
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387

Multiple sequence alignment