Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C4J85_RS25600 Genome accession   NZ_CP027760
Coordinates   5541065..5542099 (-) Length   344 a.a.
NCBI ID   WP_124410993.1    Uniprot ID   -
Organism   Pseudomonas sp. R4-34-07     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5536065..5547099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4J85_RS25570 (C4J85_5120) ruvX 5536086..5536523 (+) 438 WP_124410991.1 Holliday junction resolvase RuvX -
  C4J85_RS25575 (C4J85_5121) pyrR 5536671..5537177 (+) 507 WP_124368688.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  C4J85_RS25580 (C4J85_5122) - 5537203..5538207 (+) 1005 WP_124364800.1 aspartate carbamoyltransferase catalytic subunit -
  C4J85_RS25585 (C4J85_5123) - 5538204..5539475 (+) 1272 WP_124410992.1 dihydroorotase -
  C4J85_RS25590 (C4J85_5124) - 5539608..5540036 (-) 429 WP_124368690.1 NINE protein -
  C4J85_RS25595 (C4J85_5125) - 5540286..5540924 (+) 639 WP_124368691.1 C40 family peptidase -
  C4J85_RS25600 (C4J85_5126) pilT 5541065..5542099 (-) 1035 WP_124410993.1 type IV pilus twitching motility protein PilT Machinery gene
  C4J85_RS25605 (C4J85_5127) - 5542157..5542843 (+) 687 WP_124410994.1 YggS family pyridoxal phosphate-dependent enzyme -
  C4J85_RS25610 (C4J85_5128) proC 5542877..5543695 (+) 819 WP_124410995.1 pyrroline-5-carboxylate reductase -
  C4J85_RS25615 (C4J85_5129) - 5543721..5544308 (+) 588 WP_124364807.1 YggT family protein -
  C4J85_RS25620 (C4J85_5130) - 5544618..5545760 (+) 1143 WP_124410996.1 homoserine O-acetyltransferase -
  C4J85_RS25625 (C4J85_5131) metW 5545786..5546406 (+) 621 WP_124364809.1 methionine biosynthesis protein MetW -
  C4J85_RS25630 (C4J85_5132) - 5546428..5546862 (+) 435 WP_124372171.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37465.36 Da        Isoelectric Point: 6.9903

>NTDB_id=280279 C4J85_RS25600 WP_124410993.1 5541065..5542099(-) (pilT) [Pseudomonas sp. R4-34-07]
MDITELLTASVRRGASDLHLSAGLAPMLRIDGEVWPLDSPILAGPQLADLLSPLLNQYQQKDFETSLETDFAFELPGVAR
FRANVFQQARGVGAVFRTIPTDIQSLESLGLGEVFQRIALLPRGLVLVTGPTGSGKSTTLAAMIDYLNRHRRQHILTLED
PIEFIHRPKMALINQRQVHRDTHSFSVALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTVDRL
VDVFPAVEKAMVRSMLSESLQAVVSQVLVKKVGGGRVAAHEIMLGTPAIRNLIREDKVVQMVSAIQTGGAVGMKTLDMSL
KALVAQGVISRDDAREQARVPADI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=280279 C4J85_RS25600 WP_124410993.1 5541065..5542099(-) (pilT) [Pseudomonas sp. R4-34-07]
ATGGATATCACTGAATTACTGACGGCCAGCGTGCGCCGGGGCGCCTCCGACCTGCATTTGTCGGCCGGCCTGGCGCCGAT
GTTGCGCATCGATGGCGAGGTCTGGCCGCTGGACAGCCCGATACTGGCCGGGCCGCAGTTGGCAGATTTACTGAGCCCTT
TGCTCAACCAATACCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCGTTCGAGCTGCCCGGCGTGGCGCGC
TTTCGCGCGAATGTGTTCCAGCAGGCGCGTGGCGTTGGCGCGGTGTTTCGCACCATCCCCACCGACATCCAGAGCCTGGA
AAGCCTTGGCTTGGGCGAGGTGTTCCAGCGTATCGCGCTGTTACCCCGTGGCCTGGTGCTGGTGACCGGGCCCACCGGGT
CGGGCAAGTCCACCACGTTGGCGGCGATGATCGATTACCTGAATCGGCATCGGCGCCAGCACATCCTCACGCTGGAAGAC
CCGATTGAATTTATCCACAGGCCGAAAATGGCGTTGATCAACCAGCGCCAGGTGCATCGCGATACCCACAGCTTTTCGGT
GGCCCTGCGCTCGGCGCTACGCGAAGACCCGGATGTGATCCTGGTGGGCGAGCTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACGGCCGCTGAGACCGGCCATCTGGTATTTGGCACCTTGCACACCACCTCGGCGGCAAAGACGGTAGACCGGCTG
GTGGACGTGTTCCCGGCCGTGGAAAAGGCCATGGTCCGCTCGATGCTGTCGGAGTCGCTGCAGGCGGTGGTTTCCCAGGT
GCTGGTGAAGAAGGTCGGCGGCGGACGGGTAGCGGCTCACGAGATCATGCTGGGCACGCCGGCCATTCGTAATCTGATCC
GCGAAGACAAGGTGGTGCAGATGGTCTCGGCCATCCAGACCGGTGGGGCAGTGGGGATGAAAACGCTGGATATGAGTTTG
AAGGCGTTGGTCGCGCAGGGCGTGATCAGTCGGGACGACGCACGGGAACAGGCGAGGGTGCCGGCAGACATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

72.093

100

0.721
  pilT Pseudomonas stutzeri DSM 10701

70.93

100

0.709
  pilT Acinetobacter baumannii D1279779

69.795

99.128

0.692
  pilT Acinetobacter baumannii strain A118

69.795

99.128

0.692
  pilT Acinetobacter nosocomialis M2

69.501

99.128

0.689
  pilT Acinetobacter baylyi ADP1

68.915

99.128

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.507

100

0.657
  pilT Vibrio cholerae strain A1552

65.507

100

0.657
  pilT Legionella pneumophila strain Lp02

63.609

98.256

0.625
  pilT Legionella pneumophila strain ERS1305867

63.609

98.256

0.625
  pilT Neisseria meningitidis 8013

60.882

98.837

0.602
  pilT Neisseria gonorrhoeae MS11

60.588

98.837

0.599
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.497
  pilU Pseudomonas stutzeri DSM 10701

39.71

100

0.398
  pilU Vibrio cholerae strain A1552

39.71

100

0.398
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398

Multiple sequence alignment