Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C4J99_RS26765 Genome accession   NZ_CP027758
Coordinates   5878847..5879881 (-) Length   344 a.a.
NCBI ID   WP_124402210.1    Uniprot ID   -
Organism   Pseudomonas synxantha strain R2-54-08W     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5873847..5884881
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4J99_RS26735 (C4J99_5347) ruvX 5873880..5874317 (+) 438 WP_057022839.1 Holliday junction resolvase RuvX -
  C4J99_RS26740 (C4J99_5348) pyrR 5874451..5874957 (+) 507 WP_124402207.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  C4J99_RS26745 (C4J99_5349) - 5874982..5875986 (+) 1005 WP_034116232.1 aspartate carbamoyltransferase catalytic subunit -
  C4J99_RS26750 (C4J99_5350) - 5875983..5877254 (+) 1272 WP_124402208.1 dihydroorotase -
  C4J99_RS26755 (C4J99_5351) - 5877395..5877823 (-) 429 WP_046069932.1 TM2 domain-containing protein -
  C4J99_RS26760 (C4J99_5352) - 5878073..5878711 (+) 639 WP_124402209.1 C40 family peptidase -
  C4J99_RS26765 (C4J99_5353) pilT 5878847..5879881 (-) 1035 WP_124402210.1 type IV pilus twitching motility protein PilT Machinery gene
  C4J99_RS26770 (C4J99_5354) - 5879939..5880625 (+) 687 WP_124402211.1 YggS family pyridoxal phosphate-dependent enzyme -
  C4J99_RS26775 (C4J99_5355) proC 5880660..5881478 (+) 819 WP_124402212.1 pyrroline-5-carboxylate reductase -
  C4J99_RS26780 (C4J99_5356) - 5881504..5882091 (+) 588 WP_003195087.1 YggT family protein -
  C4J99_RS26785 (C4J99_5357) metX 5882313..5883455 (+) 1143 WP_057014171.1 homoserine O-succinyltransferase MetX -
  C4J99_RS26790 (C4J99_5358) metW 5883481..5884101 (+) 621 WP_005792273.1 methionine biosynthesis protein MetW -
  C4J99_RS26795 (C4J99_5359) - 5884123..5884557 (+) 435 WP_057022833.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37640.48 Da        Isoelectric Point: 7.6573

>NTDB_id=280231 C4J99_RS26765 WP_124402210.1 5878847..5879881(-) (pilT) [Pseudomonas synxantha strain R2-54-08W]
MDITQLLTASVSRGASDLHLSAGLAPMLRIDGEVWPMDWPRLEASQMVDLVCPLLSKHQQKDFETSLETDFAFELPGVAR
FRANVFRQDRGVGAVFRTIPTEVQNLEDLGLGEVFQRIAQLPRGLVLVTGPTGSGKSTTLAAMIDHLNQHRRQHILTLED
PIEFIHRPKKSLITQRQVHRDTHSFSVALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRL
IDVFPAGEKAMVRSMLSESLQAVVSQVLVKKVGGGRVAAHEIMLGTPAIRNLIREDKVAQLVSAIQTGGALGMKTLDMSL
KALVARGVVSRDDAREKARVPADI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=280231 C4J99_RS26765 WP_124402210.1 5878847..5879881(-) (pilT) [Pseudomonas synxantha strain R2-54-08W]
ATGGATATCACGCAATTGCTGACGGCCAGCGTAAGCCGAGGCGCCTCCGACCTGCATCTGTCGGCCGGCCTGGCGCCGAT
GCTGCGCATCGATGGCGAAGTCTGGCCCATGGATTGGCCGAGGCTTGAGGCATCGCAAATGGTCGACCTGGTGTGCCCTT
TACTGAGTAAACACCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCCTTCGAGTTGCCTGGAGTGGCGCGT
TTCCGGGCAAACGTGTTCCGCCAGGATCGCGGCGTGGGTGCGGTATTTCGCACTATCCCGACCGAGGTCCAGAACCTGGA
AGACCTGGGGCTGGGCGAGGTTTTCCAGCGTATCGCCCAGCTTCCACGCGGCCTGGTACTAGTCACTGGGCCCACCGGCT
CGGGTAAGTCCACCACCTTGGCCGCGATGATCGACCATCTTAACCAGCATCGACGCCAGCATATCCTCACCCTGGAAGAT
CCTATTGAATTTATCCACAGGCCGAAAAAATCCCTGATCACCCAACGCCAAGTGCACCGCGATACCCACAGCTTTTCGGT
CGCCCTGCGCTCGGCCCTTCGGGAAGACCCGGATGTGATACTGGTGGGTGAGCTACGCGACCTGGAAACCATTCGCCTGG
CGCTGACGGCCGCTGAGACCGGGCACTTGGTATTTGGCACCCTGCACACTTCATCGGCCGCAAAGACCGTGGACAGGCTG
ATAGACGTCTTCCCGGCCGGGGAAAAGGCCATGGTTCGCTCAATGCTGTCGGAGTCGTTGCAGGCGGTGGTGTCCCAGGT
GCTGGTGAAGAAGGTCGGCGGCGGGCGCGTGGCGGCTCATGAAATCATGCTGGGCACGCCGGCGATCCGGAATCTGATCC
GCGAGGACAAGGTGGCGCAGTTGGTCTCTGCGATTCAGACCGGTGGGGCGTTGGGAATGAAGACGCTGGATATGAGTTTG
AAGGCGTTGGTTGCGCGAGGCGTGGTCAGTCGAGACGATGCACGAGAGAAGGCGAGGGTGCCTGCAGACATATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(105-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

71.512

100

0.715
  pilT Pseudomonas stutzeri DSM 10701

70.349

100

0.703
  pilT Acinetobacter baumannii D1279779

70.088

99.128

0.695
  pilT Acinetobacter baumannii strain A118

70.088

99.128

0.695
  pilT Acinetobacter nosocomialis M2

69.795

99.128

0.692
  pilT Acinetobacter baylyi ADP1

68.328

99.128

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.579

97.965

0.642
  pilT Vibrio cholerae strain A1552

65.579

97.965

0.642
  pilT Legionella pneumophila strain Lp02

63.018

98.256

0.619
  pilT Legionella pneumophila strain ERS1305867

63.018

98.256

0.619
  pilT Neisseria meningitidis 8013

60.588

98.837

0.599
  pilT Neisseria gonorrhoeae MS11

60.294

98.837

0.596
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

100

0.503
  pilU Vibrio cholerae strain A1552

39.42

100

0.395
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395
  pilU Pseudomonas stutzeri DSM 10701

40.299

97.384

0.392

Multiple sequence alignment