Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C4K01_RS28390 Genome accession   NZ_CP027756
Coordinates   6119867..6120901 (-) Length   344 a.a.
NCBI ID   WP_057022835.1    Uniprot ID   A0AAX3IH92
Organism   Pseudomonas synxantha strain R6-28-08     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6114867..6125901
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K01_RS28360 (C4K01_5710) ruvX 6114959..6115396 (+) 438 WP_057022839.1 Holliday junction resolvase RuvX -
  C4K01_RS28365 (C4K01_5711) pyrR 6115529..6116035 (+) 507 WP_057022838.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  C4K01_RS28370 (C4K01_5712) - 6116060..6117064 (+) 1005 WP_057022837.1 aspartate carbamoyltransferase catalytic subunit -
  C4K01_RS28375 (C4K01_5713) - 6117061..6118332 (+) 1272 WP_057022836.1 dihydroorotase -
  C4K01_RS28380 (C4K01_5714) - 6118472..6118900 (-) 429 WP_046069932.1 TM2 domain-containing protein -
  C4K01_RS28385 (C4K01_5715) - 6119150..6119788 (+) 639 WP_046069933.1 C40 family peptidase -
  C4K01_RS28390 (C4K01_5716) pilT 6119867..6120901 (-) 1035 WP_057022835.1 type IV pilus twitching motility protein PilT Machinery gene
  C4K01_RS28395 (C4K01_5717) - 6120959..6121645 (+) 687 WP_043046293.1 YggS family pyridoxal phosphate-dependent enzyme -
  C4K01_RS28400 (C4K01_5718) proC 6121680..6122498 (+) 819 WP_124356345.1 pyrroline-5-carboxylate reductase -
  C4K01_RS28405 (C4K01_5719) - 6122524..6123111 (+) 588 WP_003195087.1 YggT family protein -
  C4K01_RS28410 (C4K01_5720) metX 6123253..6124395 (+) 1143 WP_046069938.1 homoserine O-succinyltransferase MetX -
  C4K01_RS28415 (C4K01_5721) metW 6124421..6125041 (+) 621 WP_005792273.1 methionine biosynthesis protein MetW -
  C4K01_RS28420 (C4K01_5722) - 6125063..6125497 (+) 435 WP_057022833.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37682.52 Da        Isoelectric Point: 7.3206

>NTDB_id=280182 C4K01_RS28390 WP_057022835.1 6119867..6120901(-) (pilT) [Pseudomonas synxantha strain R6-28-08]
MDITQLLAASVSRGASDLHLSAGLAPMLRIDGEVWPMDWPRLEASQMVDLVCPLLNKHQQKDFETSLETDFAFELPGVAR
FRANVFRQDRGVGAVFRTIPTEVQSLEDLGLGEVFQRIAQLPRGLVLVTGPTGSGKSTTLAAMIDHLNQHRRQHILTLED
PIEFIHRPKKSLITQRQVHRDTHSFSVALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRL
IDVFPAGEKAMVRSMLSESLQAVVSQVLVKKVGDGRVAAHEIMLGTPAIRNLIREDKVAQLVSAIQTGGALGMKTLDMSL
KALVARGVISRDDAREKARVPADI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=280182 C4K01_RS28390 WP_057022835.1 6119867..6120901(-) (pilT) [Pseudomonas synxantha strain R6-28-08]
ATGGATATCACGCAATTGCTGGCGGCCAGCGTAAGCCGAGGCGCCTCCGACCTGCATCTGTCGGCCGGCCTGGCGCCGAT
GCTGCGCATCGATGGCGAGGTCTGGCCCATGGATTGGCCGAGGCTTGAGGCATCGCAAATGGTCGACCTGGTGTGCCCTT
TACTGAATAAACACCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCCTTCGAGTTGCCTGGCGTGGCGCGT
TTCCGGGCAAACGTGTTCCGCCAGGATCGCGGCGTGGGTGCGGTATTTCGCACTATCCCGACCGAGGTCCAGAGCCTGGA
AGACCTGGGGCTGGGCGAGGTTTTCCAGCGTATCGCCCAGCTTCCACGCGGTCTGGTACTGGTCACTGGGCCCACCGGCT
CGGGTAAGTCCACCACCTTGGCCGCGATGATCGACCACCTTAACCAGCATCGACGCCAGCATATCCTCACCCTGGAAGAT
CCTATTGAATTTATCCACAGGCCGAAAAAATCCCTGATCACCCAGCGCCAAGTGCACCGCGATACCCACAGCTTTTCGGT
CGCCCTGCGCTCAGCCCTGCGGGAAGACCCGGATGTGATACTGGTGGGTGAGCTACGCGACCTGGAAACCATTCGCCTGG
CGCTGACGGCCGCTGAGACCGGGCACCTGGTATTTGGCACCCTGCACACTTCATCGGCAGCAAAGACCGTGGACAGGCTG
ATAGACGTCTTCCCGGCCGGGGAAAAGGCCATGGTCCGCTCAATGCTGTCGGAGTCGTTGCAGGCGGTGGTGTCCCAGGT
GCTGGTGAAGAAGGTCGGCGACGGGCGCGTGGCTGCCCATGAAATCATGCTGGGCACGCCAGCGATCCGGAACCTGATCC
GCGAAGACAAGGTGGCGCAGTTGGTTTCGGCGATTCAGACCGGTGGGGCGTTGGGAATGAAGACGCTGGATATGAGTTTG
AAGGCGTTGGTTGCGCGAGGCGTGATCAGTCGAGACGATGCGCGAGAGAAGGCGAGGGTGCCTGCAGACATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(109-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

72.093

100

0.721
  pilT Pseudomonas stutzeri DSM 10701

70.93

100

0.709
  pilT Acinetobacter baumannii D1279779

70.674

99.128

0.701
  pilT Acinetobacter baumannii strain A118

70.674

99.128

0.701
  pilT Acinetobacter nosocomialis M2

70.381

99.128

0.698
  pilT Acinetobacter baylyi ADP1

68.915

99.128

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.282

97.965

0.64
  pilT Vibrio cholerae strain A1552

65.282

97.965

0.64
  pilT Legionella pneumophila strain Lp02

63.905

98.256

0.628
  pilT Legionella pneumophila strain ERS1305867

63.905

98.256

0.628
  pilT Neisseria meningitidis 8013

61.765

98.837

0.61
  pilT Neisseria gonorrhoeae MS11

61.471

98.837

0.608
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.509
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Vibrio cholerae strain A1552

39.71

100

0.398

Multiple sequence alignment