Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C4K02_RS27635 Genome accession   NZ_CP027755
Coordinates   6063281..6064315 (-) Length   344 a.a.
NCBI ID   WP_043046292.1    Uniprot ID   A0A0D0RY29
Organism   Pseudomonas synxantha strain 2-79     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6058281..6069315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K02_RS27605 (C4K02_5552) ruvX 6058291..6058728 (+) 438 WP_003195067.1 Holliday junction resolvase RuvX -
  C4K02_RS27610 (C4K02_5553) pyrR 6058877..6059383 (+) 507 WP_043046285.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  C4K02_RS27615 (C4K02_5554) - 6059408..6060412 (+) 1005 WP_034116232.1 aspartate carbamoyltransferase catalytic subunit -
  C4K02_RS27620 (C4K02_5555) - 6060409..6061680 (+) 1272 WP_043046287.1 dihydroorotase -
  C4K02_RS27625 (C4K02_5556) - 6061824..6062252 (-) 429 WP_043046289.1 NINE protein -
  C4K02_RS27630 (C4K02_5557) - 6062502..6063140 (+) 639 WP_043046291.1 C40 family peptidase -
  C4K02_RS27635 (C4K02_5558) pilT 6063281..6064315 (-) 1035 WP_043046292.1 type IV pilus twitching motility protein PilT Machinery gene
  C4K02_RS27640 (C4K02_5559) - 6064373..6065059 (+) 687 WP_043046293.1 YggS family pyridoxal phosphate-dependent enzyme -
  C4K02_RS27645 (C4K02_5560) proC 6065094..6065912 (+) 819 WP_043046294.1 pyrroline-5-carboxylate reductase -
  C4K02_RS27650 (C4K02_5561) - 6065938..6066525 (+) 588 WP_003195087.1 YggT family protein -
  C4K02_RS27655 (C4K02_5562) - 6066720..6067862 (+) 1143 WP_043046295.1 homoserine O-acetyltransferase -
  C4K02_RS27660 (C4K02_5563) metW 6067888..6068508 (+) 621 WP_005792273.1 methionine biosynthesis protein MetW -
  C4K02_RS27665 (C4K02_5564) - 6068530..6068967 (+) 438 WP_043046296.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37562.40 Da        Isoelectric Point: 7.3205

>NTDB_id=280158 C4K02_RS27635 WP_043046292.1 6063281..6064315(-) (pilT) [Pseudomonas synxantha strain 2-79]
MDITQLLTASVSRGASDLHLSAGLAPMLRIDGEVWPMDWPRLEASQMVDLVCPLLSKHQQKDFETSLETDFAFELPGVAR
FRANVFRQDRGVGAVFRTIPTEVQSLEDLGLGEVFQRIAQLPRGLVLVTGPTGSGKSTTLAAMIDHLNQHRRQHILTLED
PIEFIHRPKMALVTQRQVHRDSHSFSVALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRL
VDVFPAGEKAMVRSMLSESLQAVVSQVLVKKVGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KALVARGVVSRDDAREKARVPGDI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=280158 C4K02_RS27635 WP_043046292.1 6063281..6064315(-) (pilT) [Pseudomonas synxantha strain 2-79]
ATGGATATCACGCAATTGCTGACGGCCAGCGTAAGCCGAGGCGCCTCCGACCTGCATCTGTCGGCCGGCCTGGCGCCGAT
GCTGCGCATCGATGGCGAAGTCTGGCCCATGGATTGGCCGAGGCTTGAGGCATCGCAAATGGTCGACCTGGTGTGCCCTT
TACTGAGTAAACACCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCCTTCGAGTTGCCTGGAGTGGCGCGT
TTCCGGGCAAACGTGTTCCGCCAGGATCGCGGCGTGGGTGCGGTATTTCGCACTATCCCGACCGAGGTCCAGAGCCTGGA
AGACTTGGGGTTGGGCGAGGTTTTCCAGCGTATCGCCCAGCTTCCACGCGGCCTGGTACTGGTCACTGGGCCCACCGGTT
CGGGTAAGTCCACCACCTTGGCGGCGATGATTGATCACCTTAACCAGCACCGACGCCAGCATATCCTCACCCTGGAAGAC
CCGATCGAATTTATCCACAGGCCGAAAATGGCCCTGGTCACCCAGCGCCAAGTGCACCGCGATAGCCACAGCTTTTCGGT
CGCCCTGCGCTCGGCCCTGCGGGAAGACCCGGATGTGATACTGGTGGGTGAGCTACGCGACCTGGAAACCATTCGCCTGG
CGCTGACGGCGGCTGAGACCGGACACCTGGTATTTGGCACTCTGCACACTTCATCGGCAGCAAAGACCGTGGACAGGCTG
GTAGACGTCTTCCCGGCCGGGGAAAAGGCCATGGTTCGCTCAATGCTGTCGGAGTCGTTGCAGGCGGTGGTGTCCCAGGT
GCTGGTGAAGAAGGTCGGCGGCGGGCGCGTGGCGGCTCATGAAATCATGCTGGGTACGCCGGCGATCCGGAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGGTCTCGGCGATCCAGACCGGTGGGGCGTTGGGAATGAAGACGCTGGATATGAGTTTG
AAGGCGTTGGTTGCGCGAGGCGTGGTCAGTCGAGACGATGCGCGAGAGAAGGCGAGGGTGCCTGGGGATATATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D0RY29

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

71.802

100

0.718
  pilT Pseudomonas stutzeri DSM 10701

71.261

99.128

0.706
  pilT Acinetobacter baumannii D1279779

69.208

99.128

0.686
  pilT Acinetobacter baumannii strain A118

69.208

99.128

0.686
  pilT Acinetobacter nosocomialis M2

68.915

99.128

0.683
  pilT Acinetobacter baylyi ADP1

67.449

99.128

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.688

97.965

0.634
  pilT Vibrio cholerae strain A1552

64.688

97.965

0.634
  pilT Legionella pneumophila strain Lp02

63.018

98.256

0.619
  pilT Legionella pneumophila strain ERS1305867

63.018

98.256

0.619
  pilT Neisseria meningitidis 8013

60

98.837

0.593
  pilT Neisseria gonorrhoeae MS11

59.706

98.837

0.59
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

99.419

0.497
  pilU Pseudomonas stutzeri DSM 10701

39.13

100

0.392
  pilU Vibrio cholerae strain A1552

39.13

100

0.392
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39

Multiple sequence alignment