Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C4K03_RS29700 Genome accession   NZ_CP027754
Coordinates   6372142..6373176 (-) Length   344 a.a.
NCBI ID   WP_124379801.1    Uniprot ID   A0A3G7UF09
Organism   Pseudomonas synxantha strain 30B     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6367142..6378176
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K03_RS29675 (C4K03_5913) pyrR 6367726..6368232 (+) 507 WP_124379796.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  C4K03_RS29680 (C4K03_5914) - 6368257..6369261 (+) 1005 WP_124379797.1 aspartate carbamoyltransferase catalytic subunit -
  C4K03_RS29685 (C4K03_5915) - 6369258..6370529 (+) 1272 WP_124379798.1 dihydroorotase -
  C4K03_RS29690 (C4K03_5916) - 6370685..6371113 (-) 429 WP_124379799.1 NINE protein -
  C4K03_RS29695 (C4K03_5917) - 6371363..6372001 (+) 639 WP_124379800.1 C40 family peptidase -
  C4K03_RS29700 (C4K03_5918) pilT 6372142..6373176 (-) 1035 WP_124379801.1 type IV pilus twitching motility protein PilT Machinery gene
  C4K03_RS29705 (C4K03_5919) - 6373234..6373920 (+) 687 WP_043046293.1 YggS family pyridoxal phosphate-dependent enzyme -
  C4K03_RS29710 (C4K03_5920) proC 6373955..6374773 (+) 819 WP_124379802.1 pyrroline-5-carboxylate reductase -
  C4K03_RS29715 (C4K03_5921) - 6374799..6375386 (+) 588 WP_003195087.1 YggT family protein -
  C4K03_RS29720 (C4K03_5922) - 6375608..6376750 (+) 1143 WP_124379803.1 homoserine O-acetyltransferase -
  C4K03_RS29725 (C4K03_5923) metW 6376776..6377396 (+) 621 WP_005792273.1 methionine biosynthesis protein MetW -
  C4K03_RS29730 (C4K03_5924) - 6377418..6377855 (+) 438 WP_124379804.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37642.54 Da        Isoelectric Point: 7.3205

>NTDB_id=280133 C4K03_RS29700 WP_124379801.1 6372142..6373176(-) (pilT) [Pseudomonas synxantha strain 30B]
MDITQLLTASVSRGASDLHLSAGLAPMLRIDGEVWPMDWPRLEASQMVDLVCPLLNKHQQKDFETSLETDFAFELPGVAR
FRANVFRQDRGVGAVFRTIPTEVQSLEDLGLGEVFQRIAQLPRGLVLVTGPTGSGKSTTMAALIDHLNQHRRQHILTLED
PIEFIHRPKMALITQRQVHRDTHGFSVALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRL
IDVFPAGEKAMVRSMLSESLQAVVSQVLVKKVGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KALVAQGVVRRDDAREKARVPGDI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=280133 C4K03_RS29700 WP_124379801.1 6372142..6373176(-) (pilT) [Pseudomonas synxantha strain 30B]
ATGGATATCACGCAATTGCTGACGGCCAGCGTAAGCCGAGGCGCCTCCGACCTGCATCTGTCGGCCGGCCTGGCGCCGAT
GCTGCGCATCGATGGCGAGGTCTGGCCCATGGATTGGCCGAGGCTTGAGGCATCGCAAATGGTCGACCTGGTGTGCCCTT
TACTGAATAAACACCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCCTTCGAGTTGCCTGGGGTGGCGCGT
TTCCGGGCAAACGTGTTCCGCCAGGATCGCGGCGTGGGCGCGGTATTCCGCACTATCCCGACCGAGGTTCAGAGCCTGGA
AGACCTGGGGCTGGGCGAGGTTTTCCAGCGTATCGCCCAGCTCCCACGCGGCCTGGTACTGGTCACCGGGCCCACCGGCT
CGGGTAAGTCCACCACCATGGCGGCATTGATCGACCACCTTAACCAGCATCGACGCCAGCATATCCTCACGCTGGAAGAC
CCGATTGAATTTATCCATCGGCCGAAAATGGCCCTGATCACCCAGCGCCAAGTGCACCGCGATACTCACGGTTTTTCGGT
CGCCCTGCGCTCGGCCCTGCGGGAAGACCCCGATGTGATACTGGTGGGTGAATTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCTGAGACCGGGCACCTGGTATTTGGCACCCTGCACACTTCATCGGCAGCAAAGACCGTGGACAGGCTG
ATAGACGTCTTCCCGGCCGGGGAAAAGGCCATGGTTCGCTCAATGCTGTCAGAGTCGTTGCAGGCGGTGGTGTCCCAAGT
GCTGGTGAAGAAGGTCGGCGGCGGACGCGTGGCGGCTCATGAAATCATGCTGGGCACGCCAGCGATCCGGAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGGTCTCGGCGATCCAGACCGGTGGGGCGTTGGGGATGAAGACGCTGGATATGAGTTTG
AAGGCGTTGGTTGCGCAGGGCGTTGTCAGGCGAGACGATGCGCGCGAGAAGGCGAGGGTGCCTGGGGATATATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G7UF09

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

71.221

100

0.712
  pilT Pseudomonas stutzeri DSM 10701

70.674

99.128

0.701
  pilT Acinetobacter baumannii D1279779

69.501

99.128

0.689
  pilT Acinetobacter baumannii strain A118

69.501

99.128

0.689
  pilT Acinetobacter nosocomialis M2

69.208

99.128

0.686
  pilT Acinetobacter baylyi ADP1

68.328

99.128

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.476

97.674

0.64
  pilT Vibrio cholerae strain A1552

65.476

97.674

0.64
  pilT Legionella pneumophila strain Lp02

62.722

98.256

0.616
  pilT Legionella pneumophila strain ERS1305867

62.722

98.256

0.616
  pilT Neisseria meningitidis 8013

60

98.837

0.593
  pilT Neisseria gonorrhoeae MS11

59.706

98.837

0.59
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.415

99.419

0.491
  pilU Pseudomonas stutzeri DSM 10701

39.13

100

0.392
  pilU Vibrio cholerae strain A1552

38.551

100

0.387
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387

Multiple sequence alignment