Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CSAC_RS05385 Genome accession   NC_009437
Coordinates   1191198..1192253 (+) Length   351 a.a.
NCBI ID   WP_011916622.1    Uniprot ID   -
Organism   Caldicellulosiruptor saccharolyticus DSM 8903     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1186198..1197253
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSAC_RS05375 (Csac_1059) - 1186204..1189404 (+) 3201 WP_011916620.1 response regulator -
  CSAC_RS05380 (Csac_1060) - 1189432..1191144 (+) 1713 WP_011916621.1 GspE/PulE family protein -
  CSAC_RS05385 (Csac_1061) pilT 1191198..1192253 (+) 1056 WP_011916622.1 type IV pilus twitching motility protein PilT Machinery gene
  CSAC_RS05390 (Csac_1062) - 1192324..1193535 (+) 1212 WP_011916623.1 type II secretion system F family protein -
  CSAC_RS05395 (Csac_1063) - 1193572..1193988 (+) 417 WP_011916624.1 type II secretion system protein -
  CSAC_RS05400 (Csac_1064) - 1194024..1194461 (+) 438 WP_011916625.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CSAC_RS05405 (Csac_1065) - 1194515..1195291 (+) 777 WP_011916626.1 A24 family peptidase -
  CSAC_RS05410 (Csac_1066) - 1195299..1195589 (+) 291 WP_011916627.1 late competence development ComFB family protein -
  CSAC_RS05415 (Csac_1067) - 1195579..1196133 (+) 555 WP_011916628.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CSAC_RS05420 (Csac_1068) - 1196130..1196702 (+) 573 WP_011916629.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39665.77 Da        Isoelectric Point: 7.2522

>NTDB_id=28013 CSAC_RS05385 WP_011916622.1 1191198..1192253(+) (pilT) [Caldicellulosiruptor saccharolyticus DSM 8903]
MDINSILKEAFLKEASDIHITPGVPPIYRIHGRLVRTDDSILTPEMVEEFVRQITNENQFKILEQKGEIDFSYSIKGVSR
FRVNVYKQRGSYSIAFRIIPVNIPPFETLGLPPVLKEFTKLNKGLVLVTGPTGSGKSTTLASLIDIINKERDVHIITLED
PIEYLHRHNKSIINQREIGSDTLSFADALRAALREDPDVILVGEMRDLETIAIALTAAETGHLVFSTLHTIGAAKTIDRI
IDVFPPHQQQQIRIQLSTVLQGVVSQQLLTRRDGKGRVVATEVMIVNPAIRNLIREAKTYQIQSIIQTHQRQGMITMEQS
LIDLYKRGLITREDAFNYATDFDFMQRLLSA

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=28013 CSAC_RS05385 WP_011916622.1 1191198..1192253(+) (pilT) [Caldicellulosiruptor saccharolyticus DSM 8903]
ATGGATATTAATTCAATTCTCAAAGAAGCATTTCTCAAGGAGGCTTCAGATATACATATTACACCAGGTGTTCCTCCAAT
TTATCGAATACACGGCAGATTAGTACGGACAGATGACTCAATTCTGACCCCTGAGATGGTGGAGGAGTTTGTGCGACAGA
TCACCAACGAAAACCAGTTTAAAATTCTTGAGCAGAAAGGTGAGATAGACTTTTCTTACAGCATAAAAGGCGTAAGCAGA
TTCAGAGTAAATGTTTATAAACAAAGAGGTTCATATTCTATAGCTTTTAGAATAATTCCAGTAAATATACCACCATTTGA
GACACTTGGCCTTCCACCGGTATTGAAGGAATTTACAAAATTGAACAAAGGACTTGTTTTAGTTACAGGTCCAACTGGTT
CGGGTAAGTCAACAACACTGGCATCGCTGATTGACATAATCAACAAAGAAAGAGATGTACATATAATCACATTAGAGGAC
CCAATAGAGTATTTGCATAGACACAACAAGAGTATTATCAATCAAAGAGAGATAGGTAGTGACACACTCAGCTTTGCAGA
CGCGCTGAGGGCGGCTTTGAGAGAAGATCCTGACGTAATCCTGGTTGGTGAGATGAGGGATTTGGAGACAATTGCGATAG
CTTTAACAGCTGCTGAAACAGGACACCTGGTGTTTTCCACACTTCACACAATTGGAGCGGCAAAGACAATAGACCGTATA
ATTGACGTTTTTCCACCGCATCAGCAACAACAGATAAGAATTCAGCTATCAACAGTTTTACAGGGAGTTGTTTCTCAGCA
ACTTTTGACCCGTCGTGATGGTAAAGGCAGGGTTGTTGCAACAGAGGTAATGATAGTAAATCCGGCCATAAGAAACCTCA
TTAGGGAGGCTAAAACGTATCAGATTCAATCAATTATTCAAACGCATCAGCGACAGGGCATGATAACAATGGAGCAGTCA
CTCATAGACTTGTACAAACGAGGGCTTATCACCCGTGAAGATGCGTTCAACTATGCTACTGACTTTGATTTTATGCAAAG
ACTGCTCAGTGCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.011

99.145

0.516
  pilT Neisseria meningitidis 8013

51.445

98.575

0.507
  pilT Neisseria gonorrhoeae MS11

50.725

98.291

0.499
  pilT Acinetobacter baumannii D1279779

52.239

95.442

0.499
  pilT Acinetobacter baumannii strain A118

52.239

95.442

0.499
  pilT Acinetobacter nosocomialis M2

52.239

95.442

0.499
  pilT Legionella pneumophila strain ERS1305867

51.622

96.581

0.499
  pilT Legionella pneumophila strain Lp02

51.622

96.581

0.499
  pilT Acinetobacter baylyi ADP1

52.424

94.017

0.493
  pilT Vibrio cholerae strain A1552

51.796

95.157

0.493
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.796

95.157

0.493
  pilT Pseudomonas aeruginosa PAK

51.343

95.442

0.49
  pilT Pseudomonas stutzeri DSM 10701

51.045

95.442

0.487
  pilU Pseudomonas stutzeri DSM 10701

41.643

100

0.419
  pilU Acinetobacter baylyi ADP1

40.882

96.866

0.396
  pilU Vibrio cholerae strain A1552

38.395

99.43

0.382

Multiple sequence alignment