Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C4J87_RS24240 Genome accession   NZ_CP027734
Coordinates   5258096..5259130 (-) Length   344 a.a.
NCBI ID   WP_124368692.1    Uniprot ID   -
Organism   Pseudomonas sp. R1-43-08     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5253096..5264130
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4J87_RS24210 (C4J87_4807) ruvX 5253178..5253615 (+) 438 WP_003213744.1 Holliday junction resolvase RuvX -
  C4J87_RS24215 (C4J87_4808) pyrR 5253763..5254269 (+) 507 WP_124368688.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  C4J87_RS24220 (C4J87_4809) - 5254295..5255299 (+) 1005 WP_124364800.1 aspartate carbamoyltransferase catalytic subunit -
  C4J87_RS24225 (C4J87_4810) - 5255296..5256567 (+) 1272 WP_124368689.1 dihydroorotase -
  C4J87_RS24230 (C4J87_4811) - 5256639..5257067 (-) 429 WP_124368690.1 NINE protein -
  C4J87_RS24235 (C4J87_4812) - 5257317..5257955 (+) 639 WP_124368691.1 C40 family peptidase -
  C4J87_RS24240 (C4J87_4813) pilT 5258096..5259130 (-) 1035 WP_124368692.1 type IV pilus twitching motility protein PilT Machinery gene
  C4J87_RS24245 (C4J87_4814) - 5259188..5259874 (+) 687 WP_124368693.1 YggS family pyridoxal phosphate-dependent enzyme -
  C4J87_RS24250 (C4J87_4815) proC 5259908..5260726 (+) 819 WP_124368694.1 pyrroline-5-carboxylate reductase -
  C4J87_RS24255 (C4J87_4816) - 5260752..5261339 (+) 588 WP_124364807.1 YggT family protein -
  C4J87_RS24260 (C4J87_4817) - 5261649..5262787 (+) 1139 Protein_4731 homoserine O-acetyltransferase -
  C4J87_RS24265 (C4J87_4818) metW 5262795..5263415 (+) 621 WP_124364809.1 methionine biosynthesis protein MetW -
  C4J87_RS24270 (C4J87_4819) - 5263437..5263871 (+) 435 WP_124368696.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37427.26 Da        Isoelectric Point: 6.9903

>NTDB_id=279660 C4J87_RS24240 WP_124368692.1 5258096..5259130(-) (pilT) [Pseudomonas sp. R1-43-08]
MDITELLTASVRRGASDLHLSAGLAPMLRIDGEVWPLDSPILAGTQVADLLSPLLNQYQQKDFETSLETDFAFELPGVAR
FRANVFQQARGVGAVFRTIPTDIQSLESLGLGEVFQRIALLPRGLVLVTGPTGSGKSTTLAAMIDYLNRHRRQHILTLED
PIEFIHRPKMALINQRQVHRDTHSFSVALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTVDRL
VDVFPAVEKAMVRSMLSESLQAVVSQVLVKKVGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGAVGMKTLDMSL
KALVAQGVISRDDAREQARVPADI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=279660 C4J87_RS24240 WP_124368692.1 5258096..5259130(-) (pilT) [Pseudomonas sp. R1-43-08]
ATGGATATCACTGAATTACTGACGGCCAGCGTGCGCCGAGGCGCCTCCGACTTGCATCTGTCGGCCGGCCTGGCGCCGAT
GCTGCGCATCGATGGCGAGGTCTGGCCGCTGGACAGCCCGATACTGGCCGGCACGCAAGTGGCGGATTTACTGAGCCCTT
TGCTCAACCAATACCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCGTTCGAGCTGCCCGGCGTGGCGCGC
TTTCGCGCGAATGTGTTCCAGCAGGCGCGTGGCGTTGGCGCAGTGTTTCGCACGATCCCCACCGACATCCAGAGCCTGGA
AAGCCTTGGCTTGGGCGAGGTGTTCCAGCGTATCGCGCTGTTACCCCGTGGCCTGGTGCTGGTGACCGGGCCCACCGGGT
CGGGCAAGTCCACCACGTTGGCGGCGATGATCGATTACCTGAATCGGCATCGGCGCCAGCACATCCTCACGCTGGAAGAC
CCCATTGAATTTATCCACAGGCCGAAAATGGCGTTGATCAACCAGCGACAGGTGCATCGCGATACCCACAGCTTTTCGGT
GGCCCTGCGCTCGGCGCTACGCGAAGACCCGGATGTGATCCTGGTGGGCGAGCTGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACGGCCGCTGAGACCGGCCATCTGGTATTTGGCACCTTGCACACCACCTCGGCGGCAAAGACGGTAGACAGGTTG
GTGGACGTGTTCCCGGCCGTGGAAAAGGCCATGGTCCGCTCGATGCTGTCGGAGTCGCTGCAGGCGGTGGTTTCCCAAGT
GCTGGTGAAGAAGGTCGGCGGCGGACGGGTAGCGGCTCACGAGATCATGCTGGGCACGCCGGCCATTCGTAATCTGATCC
GCGAAGACAAGGTGGCGCAGATGGTCTCGGCCATCCAGACCGGTGGGGCAGTGGGGATGAAAACGCTGGATATGAGTTTG
AAGGCGTTGGTCGCGCAGGGCGTGATCAGTCGGGACGACGCACGGGAACAGGCGAGGGTGCCGGCAGACATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

72.674

100

0.727
  pilT Pseudomonas stutzeri DSM 10701

71.512

100

0.715
  pilT Acinetobacter baumannii D1279779

70.381

99.128

0.698
  pilT Acinetobacter baumannii strain A118

70.381

99.128

0.698
  pilT Acinetobacter nosocomialis M2

70.088

99.128

0.695
  pilT Acinetobacter baylyi ADP1

69.501

99.128

0.689
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.087

100

0.663
  pilT Vibrio cholerae strain A1552

66.087

100

0.663
  pilT Legionella pneumophila strain Lp02

64.201

98.256

0.631
  pilT Legionella pneumophila strain ERS1305867

64.201

98.256

0.631
  pilT Neisseria meningitidis 8013

61.176

98.837

0.605
  pilT Neisseria gonorrhoeae MS11

60.882

98.837

0.602
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.497
  pilU Vibrio cholerae strain A1552

40

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

39.71

100

0.398
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398

Multiple sequence alignment