Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C4K40_RS29505 Genome accession   NZ_CP027705
Coordinates   6385747..6386781 (-) Length   344 a.a.
NCBI ID   WP_022641621.1    Uniprot ID   -
Organism   Pseudomonas sp. CMR5c     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6380747..6391781
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K40_RS29475 (C4K40_5975) ruvX 6380801..6381238 (+) 438 WP_022641615.1 Holliday junction resolvase RuvX -
  C4K40_RS29480 (C4K40_5976) pyrR 6381499..6382005 (+) 507 WP_053133958.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  C4K40_RS29485 (C4K40_5977) - 6382033..6383037 (+) 1005 WP_053133955.1 aspartate carbamoyltransferase catalytic subunit -
  C4K40_RS29490 (C4K40_5978) - 6383034..6384305 (+) 1272 WP_053133953.1 dihydroorotase -
  C4K40_RS29495 (C4K40_5979) - 6384409..6384828 (-) 420 WP_022641619.1 TM2 domain-containing protein -
  C4K40_RS29500 (C4K40_5980) - 6385079..6385702 (+) 624 WP_031320664.1 C40 family peptidase -
  C4K40_RS29505 (C4K40_5981) pilT 6385747..6386781 (-) 1035 WP_022641621.1 type IV pilus twitching motility protein PilT Machinery gene
  C4K40_RS29510 (C4K40_5982) - 6386985..6387683 (+) 699 WP_053133950.1 YggS family pyridoxal phosphate-dependent enzyme -
  C4K40_RS29515 (C4K40_5983) proC 6387717..6388535 (+) 819 WP_022641622.1 pyrroline-5-carboxylate reductase -
  C4K40_RS29520 (C4K40_5984) - 6388559..6389146 (+) 588 WP_022641623.1 YggT family protein -
  C4K40_RS29525 (C4K40_5985) - 6389146..6389439 (+) 294 WP_022641624.1 DUF167 domain-containing protein -
  C4K40_RS29530 (C4K40_5986) - 6389545..6390684 (+) 1140 WP_053133947.1 homoserine O-acetyltransferase -
  C4K40_RS29535 (C4K40_5987) metW 6390692..6391312 (+) 621 WP_022641625.1 methionine biosynthesis protein MetW -
  C4K40_RS29540 (C4K40_5988) - 6391339..6391773 (+) 435 WP_031320665.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37180.04 Da        Isoelectric Point: 7.3039

>NTDB_id=278929 C4K40_RS29505 WP_022641621.1 6385747..6386781(-) (pilT) [Pseudomonas sp. CMR5c]
MDITELLLSSVSRGASDLHLSAGLAPLLRIDGDICPLDGPVLDASQVLALIHELMSESQRKAFASQQDLDFAHELPGVAR
FRVNAFRHARGAGAVLRLIPARVQSLEELGLGSVFRQVSELPRGLVLVTGPTGSGKSTTLAAMVDHLNRTRRQHILTIED
PIEFIHPPQGCLVHQREVARDTQSFATALRAALREDPDVILLGELRDLETIRLALTAAETGHLVLATLHTSSAAKTIDRL
VDVFPGEEKALVRSMLAESLQAVIAQVLLKRIGGGRVAAHEIMLGTAAIRNLIREDKVAQMYSAIQTGGAQGMQTLDMSL
KALLAKGLISREQAREKARMPQSL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=278929 C4K40_RS29505 WP_022641621.1 6385747..6386781(-) (pilT) [Pseudomonas sp. CMR5c]
ATGGACATCACCGAATTGCTGCTGTCGAGCGTCTCGCGCGGGGCTTCCGACCTGCATCTGTCCGCTGGGCTGGCGCCGTT
GCTACGCATCGATGGCGATATCTGTCCTCTGGATGGGCCGGTACTGGATGCGTCCCAGGTGCTGGCGTTGATCCATGAAC
TGATGAGCGAGTCGCAGCGCAAGGCCTTCGCCAGCCAGCAGGACCTGGATTTCGCCCATGAGCTGCCCGGGGTGGCCAGG
TTTCGCGTCAATGCGTTCCGGCATGCCCGTGGTGCGGGAGCGGTGCTGCGGTTGATTCCTGCGCGGGTGCAGAGCCTGGA
AGAGCTCGGGCTGGGGTCGGTATTCCGGCAGGTCAGCGAGTTGCCCCGTGGGCTGGTGCTGGTGACCGGCCCCACCGGCA
GCGGCAAGTCCACCACCCTGGCGGCGATGGTCGACCACCTGAACCGGACCCGCCGCCAGCACATCCTGACCATCGAGGAC
CCCATCGAATTCATCCACCCGCCGCAAGGCTGCCTGGTGCACCAGCGTGAAGTGGCCCGCGATACCCAGAGCTTCGCCAC
GGCCTTGCGCGCGGCCCTGCGCGAGGACCCGGATGTGATCCTGCTGGGCGAGCTGCGGGACCTGGAGACCATCCGCCTGG
CGCTCACCGCTGCGGAAACCGGGCACCTGGTGCTCGCCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGATCGCTTG
GTGGATGTGTTTCCGGGAGAGGAGAAGGCCCTGGTGCGCTCGATGCTGGCCGAGTCATTGCAGGCGGTGATCGCCCAGGT
ACTGCTCAAGCGCATCGGGGGTGGAAGGGTGGCGGCCCACGAGATCATGCTGGGCACCGCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACTGGCGGGGCCCAGGGCATGCAGACCCTGGACATGAGCCTG
AAGGCATTGCTGGCAAAGGGCTTGATCAGTCGCGAACAGGCGCGGGAAAAGGCACGAATGCCCCAGAGCCTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

71.512

100

0.715
  pilT Pseudomonas stutzeri DSM 10701

69.767

100

0.698
  pilT Acinetobacter baylyi ADP1

65.407

100

0.654
  pilT Acinetobacter baumannii D1279779

64.244

100

0.642
  pilT Acinetobacter baumannii strain A118

64.244

100

0.642
  pilT Acinetobacter nosocomialis M2

63.953

100

0.64
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

63.205

97.965

0.619
  pilT Vibrio cholerae strain A1552

63.205

97.965

0.619
  pilT Legionella pneumophila strain Lp02

61.538

98.256

0.605
  pilT Legionella pneumophila strain ERS1305867

61.538

98.256

0.605
  pilT Neisseria meningitidis 8013

57.681

100

0.578
  pilT Neisseria gonorrhoeae MS11

57.391

100

0.576
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.512
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Vibrio cholerae strain A1552

40

97.384

0.39
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387

Multiple sequence alignment