Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C6Y56_RS26980 Genome accession   NZ_CP027561
Coordinates   5910410..5911444 (-) Length   344 a.a.
NCBI ID   WP_114886090.1    Uniprot ID   A0A399Q054
Organism   Pseudomonas fluorescens strain G7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5905410..5916444
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6Y56_RS26950 (C6Y56_27225) ruvX 5905646..5906083 (+) 438 WP_007953334.1 Holliday junction resolvase RuvX -
  C6Y56_RS26955 (C6Y56_27230) pyrR 5906165..5906668 (+) 504 WP_169432295.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  C6Y56_RS26960 (C6Y56_27235) - 5906696..5907700 (+) 1005 WP_108591854.1 aspartate carbamoyltransferase catalytic subunit -
  C6Y56_RS26965 (C6Y56_27240) - 5907697..5908968 (+) 1272 WP_169432296.1 dihydroorotase -
  C6Y56_RS26970 (C6Y56_27245) - 5909052..5909486 (-) 435 WP_169432297.1 TM2 domain-containing protein -
  C6Y56_RS26975 (C6Y56_27250) - 5909735..5910358 (+) 624 WP_169432298.1 C40 family peptidase -
  C6Y56_RS26980 (C6Y56_27255) pilT 5910410..5911444 (-) 1035 WP_114886090.1 type IV pilus twitching motility protein PilT Machinery gene
  C6Y56_RS26985 (C6Y56_27260) - 5911501..5912187 (+) 687 WP_169432299.1 YggS family pyridoxal phosphate-dependent enzyme -
  C6Y56_RS26990 (C6Y56_27265) proC 5912211..5913029 (+) 819 WP_085731569.1 pyrroline-5-carboxylate reductase -
  C6Y56_RS26995 (C6Y56_27270) - 5913040..5913630 (+) 591 WP_169432300.1 YggT family protein -
  C6Y56_RS27000 (C6Y56_27275) - 5913627..5913917 (+) 291 WP_169432301.1 DUF167 domain-containing protein -
  C6Y56_RS27005 (C6Y56_27280) - 5913983..5915122 (+) 1140 WP_169432302.1 homoserine O-acetyltransferase -
  C6Y56_RS27010 (C6Y56_27285) metW 5915130..5915750 (+) 621 WP_096817600.1 methionine biosynthesis protein MetW -
  C6Y56_RS27015 (C6Y56_27290) - 5915777..5916211 (+) 435 WP_169432303.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38085.76 Da        Isoelectric Point: 7.0158

>NTDB_id=278143 C6Y56_RS26980 WP_114886090.1 5910410..5911444(-) (pilT) [Pseudomonas fluorescens strain G7]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVEFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLAMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVTKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=278143 C6Y56_RS26980 WP_114886090.1 5910410..5911444(-) (pilT) [Pseudomonas fluorescens strain G7]
ATGGATATCACTGAACTGCTGGCCTTCAGCGCCAAACAGGGCGCTTCCGACCTGCACCTGTCGGCCGGTCTGCCACCGAT
GATCCGCGTGGATGGCGATGTGCGACGGATCAATCTGCCGGCGCTGGATCACAAGCAGGTGCACGAGCTGATCTACGACA
TCATGAACGACACCCAGCGAGTGGAGTTCGAGAAACATCTGGAAACCGACTTTTCCTTCGAAGTGCCCGGTGTGGCGCGG
TTTCGGGTCAATGCCTTCAACCAGAACCGTGGCGCCGGCGCTGTGTTCCGTACCATCCCGTCGAAAGTGCTGAGCATGGA
AGACCTCGCCATGGGCGACGTCTTTCGCAAAATCACCGATGCTCCGCGGGGGCTCGTGCTGGTCACCGGACCAACCGGCT
CCGGCAAATCCACGACGCTTGCGGCGATGATCGATTACCTCAATACCCACCGTCATCACCACATCCTGACCATCGAAGAC
CCCATCGAATTCGTCCACGAATCGCGTAAATGCCTGATCAATCAGCGTGAAGTCCACCGCGATACCCGCAGTTTCGCCAC
GGCATTGCGTTCAGCGCTGCGCGAAGACCCTGACGTGATCCTGGTCGGCGAGATGCGTGATCTGGAAACCATCCGCCTGG
CGCTGACGGCGGCCGAGACCGGGCACTTGGTGTTCGGCACGCTGCACACCACGTCGGCGGCCAAGACTATCGACCGGGTG
GTGGATGTGTTTCCGGGGGATGAGAAGTCCATGGTGCGCTCGATGTTGTCCGAGTCATTGCTGGCCGTGGTGTCGCAGAC
ATTGATCAAGAAGATCGGCGGCGGCCGGGTGGCGGCACACGAGATCATGCTGGGGACGTCGGCGATCCGTAACTTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGCGGGTCGCTGGGGATGCAGACGCTCGACATGTGCCTG
AAGGATCTGGTGACCAAGGGCCTGATCAGTCGTGAACATGCGCGGGAGAAGGCGCGGACACCGGATAATTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A399Q054

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

87.5

100

0.875
  pilT Pseudomonas stutzeri DSM 10701

87.5

100

0.875
  pilT Acinetobacter nosocomialis M2

79.36

100

0.794
  pilT Acinetobacter baumannii D1279779

79.36

100

0.794
  pilT Acinetobacter baumannii strain A118

79.36

100

0.794
  pilT Acinetobacter baylyi ADP1

77.326

100

0.773
  pilT Legionella pneumophila strain Lp02

72.781

98.256

0.715
  pilT Legionella pneumophila strain ERS1305867

72.781

98.256

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Acinetobacter baylyi ADP1

40.751

100

0.41
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Vibrio cholerae strain A1552

40

100

0.401

Multiple sequence alignment