Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AM478_RS08690 Genome accession   NZ_CP027539
Coordinates   1842946..1843968 (+) Length   340 a.a.
NCBI ID   WP_033649588.1    Uniprot ID   -
Organism   Serratia marcescens strain AR_0099     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1837946..1848968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM478_RS08655 (AM478_08645) - 1838280..1838720 (+) 441 WP_033649583.1 DUF29 domain-containing protein -
  AM478_RS08660 (AM478_08650) hemW 1838758..1839900 (-) 1143 WP_033635947.1 radical SAM family heme chaperone HemW -
  AM478_RS08665 (AM478_08655) - 1839893..1840486 (-) 594 WP_033649584.1 XTP/dITP diphosphatase -
  AM478_RS08670 (AM478_08660) yggU 1840504..1840794 (-) 291 WP_033649585.1 DUF167 family protein YggU -
  AM478_RS08675 (AM478_08665) - 1840791..1841345 (-) 555 WP_033635944.1 YggT family protein -
  AM478_RS08680 (AM478_08670) proC 1841377..1842198 (-) 822 WP_033649586.1 pyrroline-5-carboxylate reductase -
  AM478_RS08685 (AM478_08675) - 1842218..1842925 (-) 708 WP_033649587.1 YggS family pyridoxal phosphate-dependent enzyme -
  AM478_RS08690 (AM478_08680) pilT 1842946..1843968 (+) 1023 WP_033649588.1 type IV pilus twitching motility protein PilT Machinery gene
  AM478_RS08695 (AM478_08685) ruvX 1843965..1844387 (-) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  AM478_RS08700 (AM478_08690) - 1844387..1844950 (-) 564 WP_033635940.1 YqgE/AlgH family protein -
  AM478_RS08705 (AM478_08695) gshB 1845093..1846043 (-) 951 WP_033649589.1 glutathione synthase -
  AM478_RS08710 (AM478_08700) rsmE 1846054..1846785 (-) 732 WP_033649590.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AM478_RS08715 (AM478_08705) endA 1846855..1847550 (-) 696 WP_033649591.1 deoxyribonuclease I -
  AM478_RS08720 (AM478_08710) - 1847652..1848164 (-) 513 WP_033649592.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36451.75 Da        Isoelectric Point: 6.8349

>NTDB_id=277891 AM478_RS08690 WP_033649588.1 1842946..1843968(+) (pilT) [Serratia marcescens strain AR_0099]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANVFQQSAGMSLALRRIAGQAPSLAELAVPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSQSFDAALRAALREDPDVILLGELRDIATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRLGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGESAGEGTEG

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=277891 AM478_RS08690 WP_033649588.1 1842946..1843968(+) (pilT) [Serratia marcescens strain AR_0099]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGCCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACCGGCCGGGCGGGGAA
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCAGGGATGTCTCTCGCGTTGCGGCGCATCGCCGGGCAGGCGCCCTCGCT
TGCCGAGCTGGCGGTGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGACGACGGGCTTCTCCTAGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGTTCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGTCAAAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAACTGCGCGATATTGCCACCATTCGGC
TGGCGCTCACCGCGGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCTGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCTCGGCGGTGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGAGTGCGGGGGAGGGAACGGAGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.242

97.059

0.526
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.242

97.059

0.526
  pilT Acinetobacter baylyi ADP1

51.36

97.353

0.5
  pilT Neisseria gonorrhoeae MS11

49.851

98.529

0.491
  pilT Pseudomonas aeruginosa PAK

49.555

99.118

0.491
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Neisseria meningitidis 8013

50.301

97.647

0.491
  pilT Pseudomonas stutzeri DSM 10701

48.961

99.118

0.485
  pilT Legionella pneumophila strain ERS1305867

49.398

97.647

0.482
  pilT Legionella pneumophila strain Lp02

49.398

97.647

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.82

100

0.459
  pilU Vibrio cholerae strain A1552

40.123

95.294

0.382
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.58

95.294

0.368

Multiple sequence alignment