Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C6V80_RS02955 Genome accession   NZ_CP027432
Coordinates   534999..536066 (+) Length   355 a.a.
NCBI ID   WP_123352247.1    Uniprot ID   A0AAJ4UXP2
Organism   Caminibacter pacificus strain TB6     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 529999..541066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6V80_RS02935 (C6V80_02930) - 531527..532774 (+) 1248 WP_123352251.1 type II secretion system F family protein -
  C6V80_RS02940 (C6V80_02935) - 532775..533083 (-) 309 WP_123352250.1 AtpZ/AtpI family protein -
  C6V80_RS02945 (C6V80_02940) hemL 533403..534665 (-) 1263 WP_123352249.1 glutamate-1-semialdehyde 2,1-aminomutase -
  C6V80_RS02950 (C6V80_02945) gatC 534709..534990 (+) 282 WP_123352248.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  C6V80_RS02955 (C6V80_02950) pilT 534999..536066 (+) 1068 WP_123352247.1 type IV pilus twitching motility protein PilT Machinery gene
  C6V80_RS02960 (C6V80_02955) queA 536069..537094 (+) 1026 WP_235823174.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  C6V80_RS02965 (C6V80_02960) trxC 537150..537590 (-) 441 WP_180937085.1 thioredoxin TrxC -
  C6V80_RS02970 (C6V80_02965) - 537646..539814 (-) 2169 WP_123352246.1 endonuclease MutS2 -
  C6V80_RS02975 (C6V80_02970) - 539818..540357 (-) 540 WP_123352245.1 gamma carbonic anhydrase family protein -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 39958.25 Da        Isoelectric Point: 6.7072

>NTDB_id=277143 C6V80_RS02955 WP_123352247.1 534999..536066(+) (pilT) [Caminibacter pacificus strain TB6]
MALPFTLEQLLKSIKAYNASDLHLNVNSEPMLRIDGKLTPLNLPKLSNEQIIDLCYSVLTDKQKAILEEELELDFSFEIP
GVARFRANYYYERENLAAAYRIIPERPLSLDELKAPLIFKQLIKREKGLILVTGPTGSGKSTTLAAMLNEINEFFYKHII
TIEDPIEFVHEHKKSLFSQREVGRDTKSFIRALRASLREDPDVILIGEMRDKETIGAAITAAETGHLVFATLHTNSADQT
INRIVNVFPAEEQDQIRTQLSMALAAVISQVLLPKVGGGRIAAHEIMINNPAIANLIRENKIAQIYSQMQLNQAGTGMQT
MNQVLAEYVRKHFITKETAMAYTTKPEELRKLIGV

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=277143 C6V80_RS02955 WP_123352247.1 534999..536066(+) (pilT) [Caminibacter pacificus strain TB6]
ATGGCACTACCTTTCACACTTGAACAGTTACTGAAGAGTATAAAAGCATACAATGCGAGTGATTTGCATTTGAATGTTAA
TTCCGAGCCTATGCTAAGAATCGACGGAAAATTAACACCATTAAATTTACCAAAATTATCAAATGAACAAATTATCGATT
TGTGCTATTCGGTGCTTACCGATAAACAAAAAGCTATTTTGGAAGAAGAACTTGAGCTTGATTTTTCTTTTGAAATTCCT
GGAGTTGCGAGATTTAGGGCTAATTACTACTATGAAAGAGAAAATCTCGCCGCTGCTTACAGAATTATTCCCGAGAGACC
TTTGAGTTTGGATGAATTAAAAGCGCCTTTGATTTTTAAACAACTTATTAAAAGAGAAAAAGGTCTTATTTTAGTAACCG
GACCTACGGGAAGCGGTAAATCAACTACTCTTGCCGCTATGCTTAATGAAATTAACGAATTTTTTTATAAGCATATTATT
ACTATCGAAGACCCAATTGAGTTTGTGCACGAGCATAAAAAATCTCTTTTTTCCCAAAGAGAAGTAGGGCGTGATACGAA
ATCTTTTATTAGAGCCTTAAGAGCGTCACTCAGGGAAGACCCTGATGTTATATTAATCGGTGAGATGAGGGATAAAGAGA
CAATCGGTGCGGCTATTACGGCGGCTGAAACGGGACACCTGGTATTTGCTACATTACATACAAACAGTGCGGACCAGACG
ATAAACAGAATCGTAAACGTATTCCCGGCAGAAGAACAAGACCAGATTAGAACTCAGCTATCAATGGCGCTTGCTGCGGT
TATTTCTCAAGTACTTTTACCGAAAGTCGGAGGCGGAAGAATTGCCGCTCATGAGATTATGATAAACAATCCGGCTATTG
CAAACTTGATTCGTGAAAATAAAATTGCTCAAATTTACTCTCAAATGCAGCTAAACCAAGCGGGTACCGGAATGCAAACA
ATGAATCAGGTACTTGCCGAGTATGTTAGAAAACACTTTATCACAAAAGAAACCGCTATGGCTTATACGACTAAACCCGA
AGAGCTTAGAAAACTTATCGGAGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

56.798

93.239

0.53
  pilT Pseudomonas aeruginosa PAK

55.132

96.056

0.53
  pilT Neisseria meningitidis 8013

58.075

90.704

0.527
  pilT Neisseria gonorrhoeae MS11

57.764

90.704

0.524
  pilT Acinetobacter nosocomialis M2

56.024

93.521

0.524
  pilT Acinetobacter baumannii D1279779

56.024

93.521

0.524
  pilT Acinetobacter baumannii strain A118

56.024

93.521

0.524
  pilT Pseudomonas stutzeri DSM 10701

54.545

96.056

0.524
  pilT Legionella pneumophila strain ERS1305867

56.386

90.423

0.51
  pilT Legionella pneumophila strain Lp02

56.386

90.423

0.51
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

55.836

89.296

0.499
  pilT Vibrio cholerae strain A1552

55.836

89.296

0.499
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.405

94.648

0.468
  pilU Acinetobacter baylyi ADP1

41.691

96.62

0.403
  pilU Vibrio cholerae strain A1552

40.23

98.028

0.394
  pilU Pseudomonas stutzeri DSM 10701

39.368

98.028

0.386

Multiple sequence alignment