Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CTHE_RS05735 Genome accession   NC_009012
Coordinates   1315691..1316746 (-) Length   351 a.a.
NCBI ID   WP_011837967.1    Uniprot ID   A3DEF9
Organism   Acetivibrio thermocellus ATCC 27405     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1310691..1321746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CTHE_RS05710 (Cthe_1101) - 1310786..1312078 (-) 1293 WP_004463543.1 hypothetical protein -
  CTHE_RS05715 (Cthe_1102) - 1312078..1312629 (-) 552 WP_003515756.1 PilN domain-containing protein -
  CTHE_RS05720 (Cthe_1103) - 1312655..1313221 (-) 567 WP_011837965.1 Tfp pilus assembly protein FimT/FimU -
  CTHE_RS05725 (Cthe_1104) - 1313331..1313861 (-) 531 WP_011837966.1 type II secretion system protein -
  CTHE_RS05730 (Cthe_1105) - 1314018..1315232 (-) 1215 WP_004463551.1 type II secretion system F family protein -
  CTHE_RS05735 (Cthe_1106) pilT 1315691..1316746 (-) 1056 WP_011837967.1 type IV pilus twitching motility protein PilT Machinery gene
  CTHE_RS05740 (Cthe_1107) - 1316762..1319125 (-) 2364 WP_003515767.1 type II/IV secretion system protein -
  CTHE_RS17565 - 1319451..1319612 (-) 162 WP_157881697.1 hypothetical protein -
  CTHE_RS05750 (Cthe_1109) - 1320465..1321319 (-) 855 WP_011837968.1 permease -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38920.81 Da        Isoelectric Point: 6.9405

>NTDB_id=27563 CTHE_RS05735 WP_011837967.1 1315691..1316746(-) (pilT) [Acetivibrio thermocellus ATCC 27405]
MDINDLLRKTVENDASDLHICAGVPPIIRVYGDLINLNEPVLTPHDCVQLAKQCLSSRMYERFLEVGEVDASYSVPGVAR
FRVNVFKQRGTCAIAFRAIPTEVPSIEQLGLPGLVYDLAMKQRGLVLITGPTGHGKSTTLAAMINYINSKRRCHIVTIED
PIEYLHRHNKSIINQREIGSDTKSYANALRAVLREDPDVILIGEMRDQETIATALTAAETGHLVLSTLHTVGAAKTIDRI
IDVFPPHQQSQVRVQLSTVLMGIISQQLIKRANGKGRVLATETMVWTPAISNLIRESRTPQINTCIQTGSQFGMYTMDSC
LAELYKAGEIDYESACQYSVDMDNLRKLISN

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=27563 CTHE_RS05735 WP_011837967.1 1315691..1316746(-) (pilT) [Acetivibrio thermocellus ATCC 27405]
ATGGATATAAATGATTTACTGAGGAAGACAGTCGAAAATGATGCGTCCGACTTGCATATTTGTGCGGGGGTGCCTCCGAT
TATAAGGGTCTACGGCGATCTTATAAATTTAAACGAGCCGGTGTTAACTCCGCATGATTGTGTGCAGCTTGCCAAGCAAT
GTTTAAGCAGCAGAATGTATGAACGCTTTCTTGAAGTCGGAGAAGTCGATGCATCGTACTCGGTTCCGGGAGTGGCACGA
TTCAGGGTAAATGTTTTCAAGCAAAGAGGTACTTGTGCAATTGCATTCAGAGCAATACCTACAGAAGTTCCGTCAATTGA
GCAGCTGGGGCTTCCCGGCTTGGTATATGACCTTGCCATGAAGCAGAGAGGTCTTGTTTTGATAACGGGACCTACAGGTC
ATGGAAAGTCCACTACACTGGCTGCCATGATTAATTACATAAACAGCAAGAGAAGATGTCACATAGTTACGATTGAAGAC
CCTATAGAGTATTTGCACAGACATAACAAAAGTATTATAAATCAGAGGGAAATAGGTTCTGATACGAAATCCTACGCAAA
TGCGTTGAGAGCTGTATTAAGAGAAGACCCTGACGTTATACTTATCGGAGAGATGCGTGACCAGGAGACCATTGCAACGG
CGCTTACGGCTGCTGAAACAGGTCACTTGGTTTTGTCTACTTTGCATACCGTCGGAGCAGCAAAGACCATCGACAGAATT
ATCGACGTTTTCCCGCCTCACCAGCAGTCACAGGTAAGGGTTCAGCTTTCCACCGTGCTTATGGGTATCATATCCCAGCA
GCTTATAAAAAGAGCAAATGGCAAGGGACGTGTGCTGGCTACTGAGACCATGGTTTGGACGCCCGCTATTTCAAATCTTA
TTCGTGAAAGCCGTACACCTCAGATAAATACTTGTATTCAGACGGGTTCTCAGTTTGGAATGTATACCATGGACAGTTGC
CTTGCAGAACTCTATAAAGCCGGTGAAATTGACTATGAAAGTGCATGCCAGTATTCTGTTGATATGGATAATTTGAGAAA
ATTAATCTCAAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3DEF9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.895

97.721

0.507
  pilT Vibrio cholerae strain A1552

51.895

97.721

0.507
  pilT Neisseria meningitidis 8013

52.381

95.726

0.501
  pilT Acinetobacter nosocomialis M2

52.239

95.442

0.499
  pilT Acinetobacter baumannii D1279779

52.239

95.442

0.499
  pilT Acinetobacter baumannii strain A118

52.239

95.442

0.499
  pilT Neisseria gonorrhoeae MS11

52.083

95.726

0.499
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.573

100

0.496
  pilT Pseudomonas stutzeri DSM 10701

50.435

98.291

0.496
  pilT Pseudomonas aeruginosa PAK

51.94

95.442

0.496
  pilT Acinetobacter baylyi ADP1

51.94

95.442

0.496
  pilT Legionella pneumophila strain Lp02

51.026

97.151

0.496
  pilT Legionella pneumophila strain ERS1305867

51.026

97.151

0.496
  pilU Pseudomonas stutzeri DSM 10701

42.407

99.43

0.422
  pilU Acinetobacter baylyi ADP1

40.346

98.86

0.399
  pilU Vibrio cholerae strain A1552

38.857

99.715

0.387

Multiple sequence alignment