Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SSA_RS11355 Genome accession   NC_009009
Coordinates   2318436..2319500 (-) Length   354 a.a.
NCBI ID   WP_011837691.1    Uniprot ID   A0A3R9HKH6
Organism   Streptococcus sanguinis SK36     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2313436..2324500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SSA_RS11325 (SSA_2311) - 2313521..2314855 (-) 1335 WP_002899356.1 VWA domain-containing protein -
  SSA_RS11330 (SSA_2312) - 2314883..2315371 (-) 489 WP_011837686.1 hypothetical protein -
  SSA_RS11335 (SSA_2313) - 2315495..2315938 (-) 444 WP_011837687.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  SSA_RS11340 (SSA_2314) - 2315976..2316425 (-) 450 WP_011837688.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  SSA_RS11345 (SSA_2315) - 2316577..2317035 (-) 459 WP_011837689.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  SSA_RS11350 (SSA_2316) - 2317206..2318432 (-) 1227 WP_011837690.1 type II secretion system F family protein -
  SSA_RS11355 (SSA_2317) pilT 2318436..2319500 (-) 1065 WP_011837691.1 type IV pilus twitching motility protein PilT Machinery gene
  SSA_RS11360 (SSA_2318) - 2319502..2321184 (-) 1683 WP_002899360.1 GspE/PulE family protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39271.34 Da        Isoelectric Point: 7.0216

>NTDB_id=27553 SSA_RS11355 WP_011837691.1 2318436..2319500(-) (pilT) [Streptococcus sanguinis SK36]
MNLDELIKQAIEAGASDIHFTVGAEPSMRLHGRLTQLNNTILTNEDTVRFTKHILNEKQIAHLLEVGEVDCAYEVDNGYR
LRVNIFKQKNNCGIALRVIPKDIPSMESLGLPPVIKKLAEKRRGLILVTGPTGSGKSTTLATMINYMNSLRDEHIITIED
PIEYMHTHNKCLVNQRELGADTQSFGNALRGALRQDPDVILVGEMRDKETIEIALRAAETGHLVLSTLHTVGAVNTMDRI
IDVFPAEQQEQIRVQLSAVMEGVVSQQLMRTATGKGRVAAFEIMLGTPAIRNLIREGKTHQLLTPIQTGAQLGMITMDTS
LMGLYRRNVIDQRTLLSYSVDRAQVEKSLGIVGY

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=27553 SSA_RS11355 WP_011837691.1 2318436..2319500(-) (pilT) [Streptococcus sanguinis SK36]
ATGAATTTGGATGAGTTAATTAAGCAGGCGATTGAAGCGGGTGCATCAGATATCCACTTCACGGTTGGCGCTGAGCCAAG
CATGCGTCTGCACGGGAGGTTAACACAACTCAACAATACAATTTTGACCAACGAAGACACGGTTCGCTTCACCAAGCATA
TTTTAAATGAGAAGCAGATTGCTCATCTTTTAGAAGTCGGTGAAGTGGACTGTGCCTACGAGGTCGACAATGGCTATCGC
TTGCGGGTCAATATCTTTAAGCAGAAAAACAACTGCGGGATTGCCTTGCGGGTCATTCCCAAGGATATTCCATCTATGGA
ATCTCTGGGATTGCCACCAGTTATCAAAAAGCTGGCTGAGAAACGTCGGGGGCTGATATTGGTTACCGGACCAACAGGAA
GCGGAAAGTCAACGACCCTGGCAACCATGATCAACTACATGAATAGCTTGCGGGACGAGCACATCATCACGATTGAAGAT
CCGATCGAGTACATGCACACGCATAATAAGTGCTTGGTCAACCAACGTGAGCTGGGAGCGGATACGCAAAGCTTTGGAAA
TGCTCTCCGCGGTGCCCTTCGTCAGGACCCGGATGTCATCCTAGTCGGTGAGATGCGGGATAAAGAAACGATTGAAATCG
CTCTGCGTGCGGCGGAAACAGGTCACTTAGTCCTGTCAACTCTCCACACGGTCGGTGCTGTAAACACCATGGACCGGATT
ATTGACGTATTTCCAGCCGAGCAGCAGGAGCAGATCCGTGTACAGCTCTCCGCCGTTATGGAAGGGGTTGTATCGCAGCA
GCTTATGCGTACAGCGACCGGCAAGGGTCGTGTGGCTGCCTTTGAAATCATGCTGGGAACACCCGCTATTCGCAACCTCA
TTCGCGAAGGGAAGACCCACCAGTTGCTGACGCCAATCCAAACAGGGGCTCAGCTAGGCATGATTACCATGGACACTTCG
CTAATGGGACTCTATCGTCGCAATGTTATCGACCAAAGAACACTGCTGTCCTACTCAGTGGATCGCGCTCAAGTTGAAAA
ATCCCTAGGAATAGTGGGGTACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3R9HKH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

51.497

94.35

0.486
  pilT Acinetobacter nosocomialis M2

51.497

94.35

0.486
  pilT Acinetobacter baumannii strain A118

51.497

94.35

0.486
  pilT Vibrio cholerae strain A1552

50.445

95.198

0.48
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.445

95.198

0.48
  pilT Legionella pneumophila strain Lp02

50.898

94.35

0.48
  pilT Legionella pneumophila strain ERS1305867

50.898

94.35

0.48
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.267

96.328

0.475
  pilT Pseudomonas aeruginosa PAK

50.301

93.785

0.472
  pilT Pseudomonas stutzeri DSM 10701

50

93.785

0.469
  pilT Acinetobacter baylyi ADP1

49.849

93.503

0.466
  pilT Neisseria meningitidis 8013

49.394

93.22

0.46
  pilT Neisseria gonorrhoeae MS11

49.091

93.22

0.458
  pilU Pseudomonas stutzeri DSM 10701

44.923

91.808

0.412
  pilU Vibrio cholerae strain A1552

41.108

96.893

0.398
  pilU Acinetobacter baylyi ADP1

41.463

92.655

0.384

Multiple sequence alignment