Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C1P88_RS01335 Genome accession   NZ_CP026803
Coordinates   250336..251316 (-) Length   326 a.a.
NCBI ID   WP_001055617.1    Uniprot ID   -
Organism   Shigella flexneri strain 89-141     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 245336..256316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1P88_RS01305 - 245999..246496 (+) 498 WP_005051770.1 SprT family zinc-dependent metalloprotease -
  C1P88_RS01310 endA 246591..247298 (+) 708 WP_000286517.1 deoxyribonuclease I -
  C1P88_RS01315 rsmE 247378..248109 (+) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  C1P88_RS01320 gshB 248122..249072 (+) 951 WP_000593276.1 glutathione synthase -
  C1P88_RS01325 - 249181..249744 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  C1P88_RS01330 ruvX 249744..250160 (+) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  C1P88_RS01335 pilT 250336..251316 (-) 981 WP_001055617.1 type IV pilus twitching motility protein PilT Machinery gene
  C1P88_RS01340 yggS 251334..252038 (+) 705 WP_000997796.1 pyridoxal phosphate homeostasis protein -
  C1P88_RS01345 yggT 252056..252622 (+) 567 WP_001094819.1 osmotic shock tolerance protein YggT -
  C1P88_RS01350 yggU 252619..252909 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  C1P88_RS01355 - 252917..253510 (+) 594 WP_001174735.1 XTP/dITP diphosphatase -
  C1P88_RS01360 hemW 253503..254639 (+) 1137 WP_000239924.1 radical SAM family heme chaperone HemW -
  C1P88_RS01365 - 254953..255916 (+) 964 Protein_259 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35976.14 Da        Isoelectric Point: 5.7980

>NTDB_id=272656 C1P88_RS01335 WP_001055617.1 250336..251316(-) (pilT) [Shigella flexneri strain 89-141]
MNMEEIVAFSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=272656 C1P88_RS01335 WP_001055617.1 250336..251316(-) (pilT) [Shigella flexneri strain 89-141]
ATGAATATGGAAGAAATTGTGGCCTTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGTGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCTACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTTGCGGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Neisseria meningitidis 8013

49.085

100

0.494
  pilT Neisseria gonorrhoeae MS11

48.78

100

0.491
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.483

100

0.466
  pilT Legionella pneumophila strain ERS1305867

45.566

100

0.457
  pilT Legionella pneumophila strain Lp02

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362

Multiple sequence alignment