Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AM354_RS18870 Genome accession   NZ_CP026702
Coordinates   3957239..3958261 (+) Length   340 a.a.
NCBI ID   WP_049235391.1    Uniprot ID   -
Organism   Serratia marcescens strain AR_0027     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3952239..3963261
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM354_RS18835 (AM354_18835) - 3952579..3953013 (+) 435 WP_019456096.1 DUF29 domain-containing protein -
  AM354_RS18840 (AM354_18840) hemW 3953051..3954193 (-) 1143 WP_049235393.1 radical SAM family heme chaperone HemW -
  AM354_RS18845 (AM354_18845) - 3954186..3954779 (-) 594 WP_033635946.1 XTP/dITP diphosphatase -
  AM354_RS18850 (AM354_18850) yggU 3954797..3955087 (-) 291 WP_049235392.1 DUF167 family protein YggU -
  AM354_RS18855 (AM354_18855) - 3955084..3955638 (-) 555 WP_033635944.1 YggT family protein -
  AM354_RS18860 (AM354_18860) proC 3955670..3956491 (-) 822 WP_041036672.1 pyrroline-5-carboxylate reductase -
  AM354_RS18865 (AM354_18865) - 3956511..3957218 (-) 708 WP_033649587.1 YggS family pyridoxal phosphate-dependent enzyme -
  AM354_RS18870 (AM354_18870) pilT 3957239..3958261 (+) 1023 WP_049235391.1 type IV pilus twitching motility protein PilT Machinery gene
  AM354_RS18875 (AM354_18875) ruvX 3958258..3958680 (-) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  AM354_RS18880 (AM354_18880) - 3958680..3959243 (-) 564 WP_033635940.1 YqgE/AlgH family protein -
  AM354_RS18885 (AM354_18885) gshB 3959385..3960335 (-) 951 WP_033635939.1 glutathione synthase -
  AM354_RS18890 (AM354_18890) rsmE 3960346..3961077 (-) 732 WP_033649590.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AM354_RS18895 (AM354_18895) endA 3961147..3961842 (-) 696 WP_033649591.1 deoxyribonuclease I -
  AM354_RS18900 (AM354_18900) - 3961944..3962456 (-) 513 WP_016930082.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36432.71 Da        Isoelectric Point: 6.8881

>NTDB_id=271821 AM354_RS18870 WP_049235391.1 3957239..3958261(+) (pilT) [Serratia marcescens strain AR_0027]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANVFQQSAGMSLALRRIAGQAPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDIATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRLGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGESAGEGTEG

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=271821 AM354_RS18870 WP_049235391.1 3957239..3958261(+) (pilT) [Serratia marcescens strain AR_0027]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGCTGCACCGGCCGGGCGGGGAG
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCGGGGATGTCTCTCGCGTTGCGGCGCATCGCCGGGCAGGCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGACGACGGGCTTCTCTTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGTTCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
CGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGTGAGCTGCGCGATATCGCCACCATTCGGC
TGGCGCTCACCGCGGCGGAGACCGGTCACCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCTCGGCGGTGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGAGTGCGGGGGAGGGAACGGAGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.848

97.059

0.532
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.848

97.059

0.532
  pilT Acinetobacter baylyi ADP1

51.36

97.353

0.5
  pilT Acinetobacter baumannii strain A118

50.909

97.059

0.494
  pilT Acinetobacter nosocomialis M2

50.909

97.059

0.494
  pilT Acinetobacter baumannii D1279779

50.909

97.059

0.494
  pilT Neisseria gonorrhoeae MS11

50.149

98.529

0.494
  pilT Neisseria meningitidis 8013

50.755

97.353

0.494
  pilT Pseudomonas aeruginosa PAK

49.555

99.118

0.491
  pilT Pseudomonas stutzeri DSM 10701

48.961

99.118

0.485
  pilT Legionella pneumophila strain ERS1305867

49.398

97.647

0.482
  pilT Legionella pneumophila strain Lp02

49.398

97.647

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.82

100

0.459
  pilU Vibrio cholerae strain A1552

39.877

95.882

0.382
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.58

95.294

0.368

Multiple sequence alignment