Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NPM_RS27590 Genome accession   NZ_CP026681
Coordinates   6451578..6452705 (+) Length   375 a.a.
NCBI ID   WP_094328118.1    Uniprot ID   -
Organism   Nostoc sp. 'Peltigera membranacea cyanobiont' N6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6446578..6457705
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPM_RS27580 (NPM_5296) grpE 6448296..6449045 (-) 750 WP_094328116.1 nucleotide exchange factor GrpE -
  NPM_RS27585 (NPM_5297) pilB/pilB1 6449497..6451503 (+) 2007 WP_094328117.1 GspE/PulE family protein Machinery gene
  NPM_RS27590 (NPM_5298) pilT 6451578..6452705 (+) 1128 WP_094328118.1 type IV pilus twitching motility protein PilT Machinery gene
  NPM_RS27595 (NPM_5299) - 6452798..6454021 (+) 1224 WP_094328119.1 type II secretion system F family protein -
  NPM_RS27600 (NPM_5300) - 6454135..6454695 (+) 561 WP_094328120.1 hypothetical protein -
  NPM_RS27605 (NPM_5301) - 6454760..6455764 (-) 1005 WP_104901097.1 saccharopine dehydrogenase-like oxidoreductase -
  NPM_RS27610 - 6456062..6456310 (-) 249 WP_094328122.1 hypothetical protein -
  NPM_RS27615 (NPM_5302) - 6456746..6457237 (+) 492 WP_094328123.1 hypothetical protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41064.45 Da        Isoelectric Point: 7.4775

>NTDB_id=271733 NPM_RS27590 WP_094328118.1 6451578..6452705(+) (pilT) [Nostoc sp. 'Peltigera membranacea cyanobiont' N6]
MEMMIEDLMEQLIEMGGSDLHLSAGLPPYFRISGKLTPIGEHVLTADQCQRLIFSMLNNTQRKTLEQNWELDCSYGVKGL
ARFRVNVYKERGSYAACLRALSSKIPNFEKLGLPDIVREMTDKPRGLILVTGPTGSGKTTTLAAMIDLINRTKAEHILTV
EDPIEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIVLVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RIIDVFPHERQTQVRVQLSNSLVAVFSQTLVSKKSPKPGEYGRVMAQEILIVTPAISNLIREGKTAQIYSAIQTGGKLGM
QTLEKVLADFYKAGTISFEAAMSKTSKPDEIQRLIGTSVPPQAAGAKPGMAAKAH

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=271733 NPM_RS27590 WP_094328118.1 6451578..6452705(+) (pilT) [Nostoc sp. 'Peltigera membranacea cyanobiont' N6]
ATGGAAATGATGATTGAAGACTTGATGGAACAGTTGATTGAAATGGGTGGCTCGGATTTGCATTTATCCGCAGGTTTGCC
TCCCTACTTCCGCATCAGTGGCAAACTTACCCCCATAGGTGAGCATGTATTGACAGCCGATCAATGTCAAAGGCTGATTT
TTAGTATGCTCAACAACACCCAGCGTAAAACCTTAGAGCAGAACTGGGAATTGGATTGTTCTTATGGCGTTAAGGGTTTG
GCACGCTTCCGGGTCAATGTTTACAAAGAACGTGGTTCTTATGCTGCTTGCTTACGGGCATTAAGTTCTAAGATTCCTAA
CTTTGAAAAATTAGGTTTGCCAGATATTGTACGGGAAATGACAGATAAGCCTAGAGGACTAATTCTGGTGACAGGCCCTA
CAGGTTCCGGCAAGACAACCACCCTAGCGGCGATGATCGACTTGATTAACCGCACCAAGGCAGAGCATATTTTAACGGTG
GAAGACCCAATTGAATTTGTCTATGAACCCATTAAAAGCTTGGTTCACCAACGACAACTGGGTGAAGATACTAAGAGCTT
TGCTAATGCTTTGAAAGCAGCTTTGCGGGAAGATCCAGATATTGTTCTGGTGGGAGAAATGCGCGATTTGGAAACGATTT
CTTTGGCGATTTCCGCAGCAGAAACAGGACACTTGGTATTTGGTACTCTCCACACCAGTTCCGCCGCCCAGACAGTTGAC
CGGATTATTGACGTTTTCCCCCATGAAAGACAAACCCAGGTGCGGGTGCAGTTATCTAACTCATTAGTGGCGGTATTTAG
CCAAACCTTGGTGTCTAAGAAAAGCCCTAAACCCGGTGAATATGGTCGGGTGATGGCACAAGAAATTCTAATTGTCACTC
CCGCTATTTCTAACTTGATTAGAGAAGGTAAAACAGCCCAAATTTACTCAGCTATTCAAACTGGCGGCAAATTGGGGATG
CAAACTCTGGAGAAGGTTTTAGCTGATTTTTACAAAGCAGGAACGATTTCTTTTGAAGCGGCGATGTCTAAGACTTCTAA
GCCAGATGAAATCCAACGTCTTATCGGTACTTCTGTACCACCCCAGGCAGCAGGTGCGAAACCCGGTATGGCTGCCAAAG
CCCATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.728

93.067

0.509
  pilT Pseudomonas stutzeri DSM 10701

54.441

93.067

0.507
  pilT Acinetobacter baylyi ADP1

53.314

92.533

0.493
  pilT Legionella pneumophila strain Lp02

52.738

92.533

0.488
  pilT Legionella pneumophila strain ERS1305867

52.738

92.533

0.488
  pilT Acinetobacter baumannii strain A118

52.45

92.533

0.485
  pilT Acinetobacter nosocomialis M2

52.45

92.533

0.485
  pilT Acinetobacter baumannii D1279779

52.45

92.533

0.485
  pilT Neisseria gonorrhoeae MS11

51.032

90.4

0.461
  pilT Neisseria meningitidis 8013

50.737

90.4

0.459
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

91.2

0.456
  pilT Vibrio cholerae strain A1552

50

91.2

0.456
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.857

93.333

0.456
  pilU Acinetobacter baylyi ADP1

37.883

95.733

0.363
  pilU Pseudomonas stutzeri DSM 10701

38.244

94.133

0.36

Multiple sequence alignment