Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   NPM_RS27585 Genome accession   NZ_CP026681
Coordinates   6449497..6451503 (+) Length   668 a.a.
NCBI ID   WP_094328117.1    Uniprot ID   -
Organism   Nostoc sp. 'Peltigera membranacea cyanobiont' N6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6444497..6456503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPM_RS38780 - 6445610..6445870 (+) 261 WP_143855454.1 hypothetical protein -
  NPM_RS27575 (NPM_5295) dnaK 6446110..6448068 (-) 1959 WP_094328115.1 molecular chaperone DnaK -
  NPM_RS27580 (NPM_5296) grpE 6448296..6449045 (-) 750 WP_094328116.1 nucleotide exchange factor GrpE -
  NPM_RS27585 (NPM_5297) pilB/pilB1 6449497..6451503 (+) 2007 WP_094328117.1 GspE/PulE family protein Machinery gene
  NPM_RS27590 (NPM_5298) pilT 6451578..6452705 (+) 1128 WP_094328118.1 type IV pilus twitching motility protein PilT Machinery gene
  NPM_RS27595 (NPM_5299) - 6452798..6454021 (+) 1224 WP_094328119.1 type II secretion system F family protein -
  NPM_RS27600 (NPM_5300) - 6454135..6454695 (+) 561 WP_094328120.1 hypothetical protein -
  NPM_RS27605 (NPM_5301) - 6454760..6455764 (-) 1005 WP_104901097.1 saccharopine dehydrogenase-like oxidoreductase -
  NPM_RS27610 - 6456062..6456310 (-) 249 WP_094328122.1 hypothetical protein -

Sequence

Protein


Download         Length: 668 a.a.        Molecular weight: 74619.41 Da        Isoelectric Point: 5.1731

>NTDB_id=271732 NPM_RS27585 WP_094328117.1 6449497..6451503(+) (pilB/pilB1) [Nostoc sp. 'Peltigera membranacea cyanobiont' N6]
MTYSSPQRRSTALTTRTEFSPFGNKLVQSGYVNTEQMRQALIESRKSGRPLTEVLESITGQQLSPELLRQYKKQQLFELK
ILYGVEFLDPEVSSVANTMMGNLIETLIPVDICRRHRLVPLSKHEDQTPPSVLVAMVAPDNLEASDDLNRILRPQGLALQ
RMVITQEDYQQLINQYLDEMAVKQKHLDQEKFTDINQDLENLGNLDISDAPEEMEADLGAAMKGAEDAPVINLVNRILAK
ALHEGVSDIHIEPQEENLRIRFRKDGVLKEAFDPLPKKIIPAVTARFKIISNLDIAERRMPQDGRIRRLFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLDKLITDPETLHIVQDMVSRPFGLILVTGPTGSGKTTSLYSALSEKNDPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCSECRIPYTPTTEELARYGLSASSDVEVTFYKANTLTLDAIADAKAKNHLCPKCNG
NGYKGRCGVYEVMRVTENLQTLINEDAPTERIKEVAIEEGMKTLLAYSLDLVREGSTTLEEVERVTFTDTGLEAELKAKR
KTGLTCRSCDATLKQEWLDCPYCMTSRF

Nucleotide


Download         Length: 2007 bp        

>NTDB_id=271732 NPM_RS27585 WP_094328117.1 6449497..6451503(+) (pilB/pilB1) [Nostoc sp. 'Peltigera membranacea cyanobiont' N6]
ATGACTTACTCGTCACCACAACGGCGCAGTACCGCTCTAACTACCAGAACAGAGTTTTCGCCCTTCGGCAACAAGCTAGT
CCAATCTGGCTATGTCAATACCGAACAGATGAGGCAGGCACTAATTGAAAGCCGCAAATCTGGCAGACCCTTAACGGAAG
TACTAGAGTCAATTACTGGACAACAACTATCACCTGAGTTGCTCAGGCAATACAAAAAACAGCAGCTATTTGAACTTAAA
ATACTATACGGTGTTGAATTCCTCGATCCGGAAGTCAGTTCCGTTGCTAACACGATGATGGGGAACCTGATTGAAACCCT
CATCCCAGTGGATATTTGCCGTCGCCATCGTTTAGTACCACTGTCGAAACACGAAGACCAAACCCCGCCCTCAGTTTTAG
TGGCGATGGTTGCTCCAGATAACCTAGAGGCTTCTGATGACCTAAATCGCATCTTGCGCCCCCAAGGACTGGCATTGCAG
CGCATGGTGATTACCCAGGAAGACTACCAACAGCTAATCAACCAATATTTGGATGAAATGGCTGTTAAGCAAAAGCACCT
AGACCAAGAAAAGTTTACAGATATTAATCAGGATTTAGAAAACCTCGGAAATCTCGATATTTCCGATGCTCCTGAAGAAA
TGGAGGCCGATCTTGGGGCAGCGATGAAGGGTGCAGAGGATGCCCCAGTCATCAACCTAGTGAACAGAATCCTGGCTAAA
GCCTTGCATGAGGGCGTTTCTGACATTCATATCGAACCGCAAGAAGAAAACTTACGCATTCGCTTTCGGAAGGATGGCGT
ACTGAAGGAAGCTTTCGATCCCCTACCGAAAAAAATCATCCCGGCGGTGACAGCCCGATTTAAAATCATCTCGAATCTAG
ATATTGCCGAACGCCGTATGCCCCAAGATGGACGCATCCGGCGGCTATTTGAGGGACGTAAGGTGGATTTCCGCGTGAAT
ACCTTGCCCAGTCGCTACGGGGAAAAGGTGGTACTGCGGATTTTGGATAACTCCTCTACCCAACTGGGATTAGATAAGTT
AATTACCGATCCAGAGACTTTACATATTGTCCAGGATATGGTTAGCCGTCCCTTTGGACTAATTTTGGTAACTGGGCCAA
CTGGTTCTGGGAAAACAACCTCGCTCTATTCTGCACTATCAGAAAAGAACGATCCCGGAATTAATATCAGTACTGTGGAA
GACCCAATTGAGTACAGTCTTCCAGGGATTACTCAAGTACAGGTGATTCGAGAAAAAGGGTTAGATTTTGCTACCGCTCT
GCGGGCTTTCTTGCGGCAAGATCCAGATGTGCTACTGGTGGGTGAAACGCGGGATAAGGAAACGGCAAAAACAGCAATTG
AGGCTGCCTTGACCGGTCACTTAGTATTAACTACCTTACATACCAATGATGCCCCAGGTGCGATCGCTCGTTTGGGAGAA
ATGGGCATTGAACCTTTCATGGTTTCTAGTTCTCTAATTGGCGTTCTAGCTCAACGTTTGGTGCGGCGCGTATGTTCTGA
ATGTCGGATTCCCTACACTCCCACAACCGAGGAACTAGCTCGTTATGGTCTATCAGCTTCTTCTGATGTGGAAGTCACCT
TCTATAAAGCTAACACTTTGACATTAGATGCGATCGCAGATGCCAAAGCCAAAAATCACCTTTGCCCAAAATGTAATGGC
AACGGCTATAAAGGGCGTTGTGGTGTTTATGAAGTTATGCGAGTCACCGAAAATCTGCAAACTCTCATTAACGAAGATGC
ACCCACAGAACGCATCAAGGAAGTGGCAATAGAAGAGGGGATGAAAACCTTGCTGGCTTATAGTTTGGACTTAGTGCGCG
AAGGTTCCACGACTTTAGAAGAAGTAGAACGAGTGACGTTTACTGATACTGGCTTGGAAGCAGAGTTAAAAGCCAAACGC
AAGACTGGTCTTACCTGTCGGAGTTGCGATGCCACATTGAAACAAGAATGGCTGGATTGTCCCTACTGTATGACATCTCG
GTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(64-181)

Type II/IV secretion system protein (T2SSE)

(236-503)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.667

100

0.671

Multiple sequence alignment