Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C4Q26_RS10220 Genome accession   NZ_CP026674
Coordinates   2171560..2172558 (-) Length   332 a.a.
NCBI ID   WP_104883049.1    Uniprot ID   -
Organism   Pseudomonas sp. SWI44     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2166560..2177558
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4Q26_RS10200 (C4Q26_10200) argS 2167792..2169528 (+) 1737 WP_023378217.1 arginine--tRNA ligase -
  C4Q26_RS10205 (C4Q26_10205) - 2169530..2170228 (+) 699 WP_023378216.1 SPOR domain-containing protein -
  C4Q26_RS10210 (C4Q26_10210) - 2170270..2170689 (-) 420 WP_023378215.1 TM2 domain-containing protein -
  C4Q26_RS10215 (C4Q26_10215) - 2170858..2171484 (+) 627 WP_023378214.1 C40 family peptidase -
  C4Q26_RS10220 (C4Q26_10220) pilT 2171560..2172558 (-) 999 WP_104883049.1 type IV pilus twitching motility protein PilT Machinery gene
  C4Q26_RS10225 (C4Q26_10225) - 2172624..2173310 (+) 687 WP_023378212.1 YggS family pyridoxal phosphate-dependent enzyme -
  C4Q26_RS10230 (C4Q26_10230) proC 2173349..2174167 (+) 819 WP_023378211.1 pyrroline-5-carboxylate reductase -
  C4Q26_RS10235 (C4Q26_10235) - 2174178..2174768 (+) 591 WP_023378210.1 YggT family protein -
  C4Q26_RS10240 (C4Q26_10240) - 2174858..2175997 (+) 1140 WP_027907907.1 homoserine O-acetyltransferase -
  C4Q26_RS10245 (C4Q26_10245) metW 2176005..2176625 (+) 621 WP_023378208.1 methionine biosynthesis protein MetW -
  C4Q26_RS10250 (C4Q26_10250) - 2176647..2177081 (+) 435 WP_023378207.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 35683.28 Da        Isoelectric Point: 6.6988

>NTDB_id=271572 C4Q26_RS10220 WP_104883049.1 2171560..2172558(-) (pilT) [Pseudomonas sp. SWI44]
MDVTDLLARAMAAGASDLHLAAGDVPLIRLDGELQRLDLPPLSATALHDGLAPLLAEAQCRQWAQGDELDLALDLPSLGR
IRLNLFRQSNGPAVAMRIIAQRIATLEALGLTEVFRAFAQHTDGLVLVGGPTGSGKSSTLAALVDQLNRERSLHIITLED
PIEVIHSCQRSLVNQREVGRHCRDFAQGLRSALRQDPDVIVIGELRDLETIRLALRAAETGHLVLATVHTRSAINSIDRL
VEVFAAEEKPLVRAMLAESLRLVVAQVLVKRRGGGRVAAREVLVATPAVRNLIREGRAAQLQSVMQSGAAAGMKTMEGAM
EALRAEGLIDAL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=271572 C4Q26_RS10220 WP_104883049.1 2171560..2172558(-) (pilT) [Pseudomonas sp. SWI44]
ATGGACGTGACTGACCTCTTGGCCCGGGCGATGGCTGCAGGGGCGAGTGACCTGCACCTGGCCGCGGGCGACGTACCGCT
GATTCGCCTGGATGGCGAGCTGCAACGCCTGGATCTGCCGCCGTTGTCGGCGACGGCGCTGCACGACGGGCTGGCGCCTT
TGCTGGCCGAAGCGCAGTGTCGCCAGTGGGCGCAAGGTGATGAGCTGGACCTGGCGCTCGATCTCCCCTCGCTTGGGCGT
ATCCGCTTGAATCTGTTTCGCCAGTCGAATGGGCCAGCGGTGGCCATGCGGATCATTGCGCAACGAATCGCCACACTCGA
AGCGTTAGGCCTGACAGAAGTGTTTCGAGCTTTTGCCCAACATACCGATGGCCTTGTCCTGGTAGGTGGGCCGACCGGTA
GCGGGAAGTCCAGCACGCTGGCCGCGCTGGTCGACCAGCTCAATCGCGAGCGCTCGCTGCACATCATCACATTGGAAGAC
CCTATCGAAGTTATCCACAGTTGCCAGCGCAGCCTGGTCAACCAGCGCGAGGTCGGACGCCACTGTCGTGACTTCGCCCA
GGGGTTGCGCAGTGCACTGCGCCAAGACCCGGATGTCATCGTGATCGGCGAACTGAGGGATCTCGAAACGATTCGTCTGG
CGCTCCGGGCTGCCGAGACGGGGCATCTGGTGCTGGCGACCGTCCATACCCGGTCGGCGATCAACAGCATCGACCGTCTG
GTGGAGGTGTTTGCCGCCGAGGAGAAGCCGTTGGTCAGGGCCATGCTGGCCGAATCGCTGCGCTTGGTGGTAGCGCAGGT
GCTGGTGAAACGAAGAGGTGGCGGCCGGGTGGCGGCGCGGGAAGTGTTGGTGGCGACGCCCGCTGTGCGTAACCTGATCC
GAGAGGGGCGAGCGGCGCAGTTGCAGTCCGTGATGCAGTCGGGGGCGGCGGCGGGGATGAAGACGATGGAAGGAGCGATG
GAGGCGTTGAGGGCAGAAGGGCTGATCGACGCGTTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.368

99.096

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.057

99.699

0.509
  pilT Vibrio cholerae strain A1552

51.057

99.699

0.509
  pilT Acinetobacter baylyi ADP1

50.909

99.398

0.506
  pilT Pseudomonas stutzeri DSM 10701

50.76

99.096

0.503
  pilT Acinetobacter baumannii strain A118

50.456

99.096

0.5
  pilT Acinetobacter baumannii D1279779

50.456

99.096

0.5
  pilT Acinetobacter nosocomialis M2

50.152

99.096

0.497
  pilT Legionella pneumophila strain Lp02

48.936

99.096

0.485
  pilT Legionella pneumophila strain ERS1305867

48.936

99.096

0.485
  pilT Neisseria meningitidis 8013

48.485

99.398

0.482
  pilT Neisseria gonorrhoeae MS11

48.182

99.398

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.364

99.398

0.461
  pilU Pseudomonas stutzeri DSM 10701

38.182

99.398

0.38
  pilU Vibrio cholerae strain A1552

37.654

97.59

0.367
  pilF Thermus thermophilus HB27

31.51

100

0.364

Multiple sequence alignment