Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C3F22_RS10835 Genome accession   NZ_CP026420
Coordinates   2219647..2220768 (+) Length   373 a.a.
NCBI ID   WP_005234318.1    Uniprot ID   A0A2L0I6Q7
Organism   Acinetobacter sp. ACNIH1     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2214647..2225768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C3F22_RS10820 (C3F22_10830) - 2216631..2217737 (+) 1107 WP_005234320.1 acyltransferase family protein -
  C3F22_RS10825 (C3F22_10835) - 2217769..2218458 (-) 690 WP_005234319.1 YggS family pyridoxal phosphate-dependent enzyme -
  C3F22_RS10830 (C3F22_10840) pilT 2218582..2219619 (+) 1038 WP_004783232.1 type IV pilus twitching motility protein PilT Machinery gene
  C3F22_RS10835 (C3F22_10845) pilU 2219647..2220768 (+) 1122 WP_005234318.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C3F22_RS10840 (C3F22_10850) fur 2220836..2221273 (-) 438 WP_005234317.1 ferric iron uptake transcriptional regulator -
  C3F22_RS10845 (C3F22_10855) - 2221386..2221784 (+) 399 WP_004783238.1 outer membrane protein assembly factor BamE -
  C3F22_RS10850 (C3F22_10860) - 2221888..2222217 (-) 330 WP_104426548.1 RnfH family protein -
  C3F22_RS10855 (C3F22_10865) - 2222217..2223296 (-) 1080 WP_104426549.1 hypothetical protein -
  C3F22_RS10860 (C3F22_10870) - 2223475..2223930 (-) 456 WP_004783243.1 bacteriohemerythrin -
  C3F22_RS10865 (C3F22_10875) - 2224226..2225074 (-) 849 WP_104426550.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41716.99 Da        Isoelectric Point: 6.8892

>NTDB_id=269233 C3F22_RS10835 WP_005234318.1 2219647..2220768(+) (pilU) [Acinetobacter sp. ACNIH1]
MDFNGLLNYMVEKKASDLFITADVEPSIKINGQIVPIGSVKLPGAAVGQLLNSIMTEKQRKEFADTRECNFAISNPDKSA
RFRVSAFQQRDQPGMVLRRIETVIPTMDELKLPPILKELAMTKRGIIIFVGATGTGKSTSLASLIGYRNENSKGHIITIE
DPIEFVHQHKGCIITQREVGIDTDSFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEVDRHSQLFMDLSLNMKAIVAQQLIPTIDGNGRRAAIEILINSPLIADLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALYDLYKAKQISYKDALKHADSPNDLRLQIKLSEEGGEHLLRAHSNITFDGQT

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=269233 C3F22_RS10835 WP_005234318.1 2219647..2220768(+) (pilU) [Acinetobacter sp. ACNIH1]
ATGGACTTTAACGGCTTACTCAACTATATGGTAGAGAAAAAGGCCTCGGATCTGTTCATTACAGCCGATGTTGAACCCTC
TATCAAGATTAACGGTCAGATTGTACCGATTGGCTCGGTAAAATTGCCGGGCGCGGCTGTGGGGCAGCTACTTAACTCAA
TCATGACCGAGAAGCAGCGCAAGGAATTTGCTGACACACGTGAGTGTAACTTCGCCATCAGCAACCCCGACAAATCGGCA
CGTTTCCGTGTTAGTGCTTTTCAGCAACGTGATCAGCCAGGCATGGTGCTGCGCCGTATTGAAACCGTCATTCCGACTAT
GGATGAGCTGAAGTTACCGCCTATTCTAAAAGAACTGGCGATGACCAAACGCGGAATCATTATTTTTGTAGGTGCAACCG
GTACGGGTAAATCTACTTCCCTCGCCTCTTTGATTGGCTACCGTAATGAAAATTCCAAAGGTCATATCATTACCATTGAA
GACCCGATCGAATTTGTGCACCAGCATAAGGGCTGTATCATCACCCAGCGCGAAGTCGGCATTGATACTGACTCCTTTGA
AGTGGCATTGAAAAATACCTTACGTCAGGCACCAGATGTGATTCTGATTGGTGAGATTCGTTCTCGCGAAACCATGGACT
ATGCGATTGCCTTTGCGGAAACCGGTCACCTGGTTTTTGCGACCCTGCACGCCAACAACGCTAACCAGGCCATCGACCGT
ATCATTCACTTCTTCGAAGTCGACCGTCACAGCCAGCTGTTTATGGATCTGTCCCTCAACATGAAAGCCATTGTGGCACA
GCAATTGATTCCTACCATTGATGGTAATGGCCGCCGTGCAGCGATTGAAATCCTGATCAATTCGCCATTGATTGCGGATC
TGATCCGTAAAGGTGATGTGCATGAGATCAAGGATCTGATGAAACGTTCACGTGAACTGGGCATGCAAACCTTCGACCAG
GCATTATATGACCTGTATAAGGCCAAGCAGATTAGCTATAAAGATGCACTAAAACATGCTGACTCACCAAACGACCTGCG
TCTGCAAATCAAACTGTCAGAAGAAGGTGGAGAGCATTTATTACGTGCGCATTCCAATATTACTTTTGATGGTCAAACCT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L0I6Q7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

72.751

100

0.737
  pilU Pseudomonas stutzeri DSM 10701

68.464

99.464

0.681
  pilU Vibrio cholerae strain A1552

57.835

94.102

0.544
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.775

92.761

0.397
  pilT Legionella pneumophila strain Lp02

42.857

90.08

0.386
  pilT Legionella pneumophila strain ERS1305867

42.857

90.08

0.386
  pilT Acinetobacter nosocomialis M2

41.691

91.957

0.383
  pilT Acinetobacter baumannii D1279779

41.691

91.957

0.383
  pilT Acinetobacter baumannii strain A118

41.691

91.957

0.383
  pilT Pseudomonas aeruginosa PAK

40.462

92.761

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.601

94.102

0.373
  pilT Vibrio cholerae strain A1552

39.601

94.102

0.373
  pilT Acinetobacter baylyi ADP1

40.233

91.957

0.37
  pilT Neisseria meningitidis 8013

39.884

92.761

0.37
  pilT Neisseria gonorrhoeae MS11

39.884

92.761

0.37
  pilT Pseudomonas stutzeri DSM 10701

39.306

92.761

0.365

Multiple sequence alignment