Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C3F34_RS03555 Genome accession   NZ_CP026412
Coordinates   767240..768358 (+) Length   372 a.a.
NCBI ID   WP_044739263.1    Uniprot ID   A0A5N4WAN1
Organism   Acinetobacter sp. ACNIH2     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 762240..773358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C3F34_RS03535 (C3F34_03535) - 762476..763501 (+) 1026 WP_016165371.1 class I SAM-dependent methyltransferase -
  C3F34_RS03540 (C3F34_03540) - 763511..764974 (+) 1464 WP_044739265.1 amino acid permease -
  C3F34_RS03545 (C3F34_03545) - 765363..766049 (-) 687 WP_044739264.1 YggS family pyridoxal phosphate-dependent enzyme -
  C3F34_RS03550 (C3F34_03550) pilT 766173..767210 (+) 1038 WP_016165374.1 type IV pilus twitching motility protein PilT Machinery gene
  C3F34_RS03555 (C3F34_03555) pilU 767240..768358 (+) 1119 WP_044739263.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C3F34_RS03560 (C3F34_03560) omp38 768501..769517 (-) 1017 WP_044739262.1 outer membrane protein Omp38 -
  C3F34_RS03565 (C3F34_03565) - 769895..770413 (+) 519 WP_016165377.1 GspH/FimT family pseudopilin -
  C3F34_RS03570 (C3F34_03570) - 770515..771690 (+) 1176 WP_044739261.1 thiolase family protein -
  C3F34_RS03575 (C3F34_03575) - 771833..772279 (+) 447 WP_016165379.1 VOC family protein -
  C3F34_RS03580 (C3F34_03580) - 772382..772999 (+) 618 WP_044739260.1 rhomboid family intramembrane serine protease -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41843.18 Da        Isoelectric Point: 6.8365

>NTDB_id=269166 C3F34_RS03555 WP_044739263.1 767240..768358(+) (pilU) [Acinetobacter sp. ACNIH2]
MDFNDLLNLMIQQKASDLFVTAEVEPSMKINGQIVPVAKTKLTGEIVGQLLNSIMSDKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDQPGMVLRRIETKIPTMDELKLPPVLKDLAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHGQLFMDLSLNLKAMVAQQLIPTIDGNSRRAAIEILINSPLISDLIRKGDIHEIKDLMKRSRELGMQTFDQ
ALYDLYKAKQITYKDALKHADSPNDLRLQIKLSEEGADRLLNASSHITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=269166 C3F34_RS03555 WP_044739263.1 767240..768358(+) (pilU) [Acinetobacter sp. ACNIH2]
ATGGACTTTAACGACTTACTCAATCTCATGATTCAACAAAAAGCATCTGACCTTTTTGTCACAGCTGAAGTTGAACCATC
TATGAAAATTAATGGTCAAATTGTTCCTGTTGCGAAAACCAAGTTAACAGGTGAAATTGTTGGTCAACTCTTAAATTCAA
TTATGAGTGATAAACAACGTAAAGAATTTGCAGATACACGTGAATGTAACTTTGCGATTATGAATCGTGAAAAAACAGCA
CGTTTCCGTGTCAGTGCATTTCAACAACGTGACCAACCAGGCATGGTTCTACGTCGCATTGAAACTAAAATTCCAACCAT
GGACGAACTCAAGCTTCCTCCAGTGTTGAAAGACTTAGCCATGACCAAACGTGGCATCATTATTTTTGTGGGTGCAACAG
GTACTGGTAAATCTACTTCCTTGGCCTCAATTATTGGATATCGTAATCAAAATTCTAAAGGTCATATCATTACCATTGAA
GATCCGATCGAATTTATTCACGAACATGCAGGCTGTATTATCACTCAACGTGAAGTAGGCATCGATACCGACTCCTTTGA
AATTGCGTTGAAAAATACCTTACGTCAAGCACCCGATGTTATTCTGATTGGTGAGATTCGTTCACGCGAGACCATGGATT
ACGCAATTGCTTTCGCAGAAACAGGCCATTTGGTTTTTGCAACGCTACATGCTAACAATGCCAACCAAGCGATTGATCGT
ATCATTCACTTCTTTGAAGCAGACCGTCATGGACAATTATTCATGGACTTGTCCTTAAATTTGAAAGCCATGGTCGCTCA
GCAACTCATTCCAACGATTGATGGTAATTCACGCCGTGCTGCAATTGAGATTTTGATTAATTCACCATTAATTTCTGACT
TAATCCGAAAAGGCGATATTCACGAAATTAAAGATTTAATGAAGCGTTCACGTGAACTCGGTATGCAAACCTTTGACCAA
GCACTTTACGATTTATATAAAGCCAAGCAGATCACTTATAAAGATGCGCTGAAACATGCCGATTCACCAAATGATTTACG
CCTACAAATCAAACTCTCTGAGGAAGGTGCTGATCGTTTATTGAATGCAAGCTCGCATATTACTTTTGATGGTCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5N4WAN1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.19

100

0.774
  pilU Pseudomonas stutzeri DSM 10701

67.925

99.731

0.677
  pilU Vibrio cholerae strain A1552

55.84

94.355

0.527
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

93.011

0.395
  pilT Pseudomonas aeruginosa PAK

40.173

93.011

0.374
  pilT Acinetobacter baumannii strain A118

39.65

92.204

0.366
  pilT Acinetobacter baumannii D1279779

39.65

92.204

0.366
  pilT Acinetobacter nosocomialis M2

39.65

92.204

0.366
  pilT Legionella pneumophila strain ERS1305867

40.476

90.323

0.366
  pilT Legionella pneumophila strain Lp02

40.476

90.323

0.366
  pilT Acinetobacter baylyi ADP1

39.359

92.204

0.363
  pilT Neisseria meningitidis 8013

39.706

91.398

0.363
  pilT Neisseria gonorrhoeae MS11

39.706

91.398

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

38.177

94.355

0.36
  pilT Vibrio cholerae strain A1552

38.177

94.355

0.36
  pilT Pseudomonas stutzeri DSM 10701

38.728

93.011

0.36

Multiple sequence alignment